LOCUS       Exported               12111 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21.
ACCESSION   .
VERSION     .
KEYWORDS    pSMPP-mCherry-hTRIM21
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12111)
  AUTHORS   Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, 
            Schuh M
  TITLE     A Method for the Acute and Rapid Degradation of Endogenous Proteins.
  JOURNAL   Cell. 2017 Nov 13. pii: S0092-8674(17)31255-2. doi: 
            10.1016/j.cell.2017.10.033.
  PUBMED    29153837
REFERENCE   2  (bases 1 to 12111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12111
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(25..43)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(26..43)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(333..352)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(566..585)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     685..707
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(745..763)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        831..935
                     /gene="bla"
                     /label=AmpR promoter
     CDS             936..1796
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1154..1173)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1967..2555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2456..2475
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2709..2726
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2796..2816)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2801..3130
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2981..3116
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3043..3062
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          4264..4329
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4459..4479
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4904..5038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4941..4960)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4995..5014
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5207..5840
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5887..6012
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6504..6737
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6922..6966
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7251..7368
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7521..7928
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      7937..7946
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7943..8650
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8088..8106)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8356..8375
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8550..8573
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     regulatory      8657..8666
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(10203..10223)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10621..10632)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10664..11163
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10699..10722)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11096..11115
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11184..11783
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11184..11203)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11680..11700
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             11878..12111
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
       61 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
      121 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
      181 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
      241 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
      301 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
      361 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
      421 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
      481 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
      541 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
      601 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      661 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
      721 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      781 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      841 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      901 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      961 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1021 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1081 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1141 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     1201 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1261 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1321 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1381 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1441 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1501 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1561 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1621 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1681 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1741 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1801 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1861 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1921 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1981 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2041 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2101 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2161 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2221 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2281 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2341 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2401 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2461 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2521 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2581 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2641 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2701 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2761 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     2821 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2881 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2941 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3001 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3061 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3121 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     3181 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     3241 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     3301 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     3361 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     3421 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
     3481 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     3541 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     3601 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     3661 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     3721 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     3781 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     3841 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     3901 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     3961 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     4021 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
     4081 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
     4141 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
     4201 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     4261 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     4321 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     4381 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     4441 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     4501 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     4561 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     4621 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
     4681 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     4741 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
     4801 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     4861 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     4921 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4981 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     5041 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     5101 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     5161 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     5221 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     5281 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     5341 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     5401 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     5461 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     5521 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     5581 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     5641 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5701 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5761 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5821 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     5881 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     5941 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     6001 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     6061 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     6121 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     6181 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     6241 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     6301 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc gccgctgatc
     6361 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     6421 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     6481 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     6541 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     6601 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     6661 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     6721 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     6781 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     6841 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     6901 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     6961 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     7021 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     7081 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     7141 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     7201 ttagtgaacg gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga
     7261 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     7321 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     7381 agggacagca gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga
     7441 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     7501 ggtttggcaa gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa
     7561 gaatagagaa gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag
     7621 gatatctgcg gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag
     7681 tttcggcccc ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca
     7741 gtttcttaag acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc
     7801 cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg
     7861 agctctataa aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt
     7921 cgcccggggg ggatccgcca ccatggtgag caagggcgag gaggacaaca tggccatcat
     7981 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     8041 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     8101 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     8161 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     8221 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     8281 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     8341 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     8401 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     8461 ggacggcggc cactacgacg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca
     8521 gctgcccggc gcctacaacg tcaacatcaa gctggacatc acctcccaca acgaggacta
     8581 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg gcatggacga
     8641 gctgtacaag acgcgtgcca ccatggcttc agcagcacgc ttgacaatga tgtgggagga
     8701 ggtcacatgc cctatctgcc tggacccctt cgtggagcct gtgagcatcg agtgtggcca
     8761 cagcttctgc caggaatgca tctctcaggt tgggaaaggt gggggcagcg tctgtcctgt
     8821 gtgccggcag cgctttctgc tcaagaatct ccggcccaat cgacagctag ccaacatggt
     8881 gaacaacctt aaagaaatca gccaggaggc cagagagggc acacaggggg aacggtgtgc
     8941 agtgcatgga gagagacttc acctgttctg tgagaaagat gggaaggccc tttgctgggt
     9001 atgtgcccag tctcggaaac accgtgacca cgccatggtc cctcttgagg aggctgcaca
     9061 ggagtaccag gagaagctcc aggtggcatt aggggaactg agaagaaagc aggagttggc
     9121 tgagaagttg gaagtggaaa ttgcaataaa gagagcagac tggaagaaaa cagtggaaac
     9181 acagaaatct aggattcacg cagagtttgt gcagcaaaaa aacttcctgg ttgaagaaga
     9241 acagaggcag ctgcaggagc tggagaagga tgagagggag cagctgagaa tcctggggga
     9301 gaaagaggcc aagctggccc agcagagcca ggccctacag gagctcatct cagagctaga
     9361 tcgaaggtgc cacagctcag cactggaact gctgcaggag gtgataattg tcctggaaag
     9421 gagtgagtcc tggaacctga aggacctgga tattacctct ccagaactca ggagtgtgtg
     9481 ccatgtgcca gggctgaaga agatgctgag gacatgtgca gtccacatca ctctggatcc
     9541 agacacagcc aatccgtggc tgatactttc agaagatcgg agacaagtga ggcttggaga
     9601 cacccagcag agcatacctg gaaatgaaga gagatttgat agttatccta tggtcctggg
     9661 tgcccagcac tttcactctg gaaaacatta ctgggaggta gatgtgacag gaaaggaggc
     9721 ctgggacctg ggtgtctgca gagactctgt gcgcaggaag gggcactttt tgcttagttc
     9781 caagagtggc ttctggacaa tttggttgtg gaacaaacaa aaatatgagg ctggcaccta
     9841 cccccagact cccctccacc ttcaggtgcc tccatgccaa gttgggattt tcctggacta
     9901 tgaggctggc atggtctcct tctacaacat cactgaccat ggctccctca tctactcctt
     9961 ctctgaatgt gcctttacag gacctctgcg gcccttcttc agtcctggtt tcaatgatgg
    10021 aggaaaaaac acagcccctc taaccctctg tccactgaat attggatcac aaggatccac
    10081 tgactattga gcggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag
    10141 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
    10201 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    10261 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    10321 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    10381 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    10441 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    10501 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
    10561 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    10621 tcggccctca atccagcgga ccttccttcc catcaattct accgggtagg ggaggcgctt
    10681 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
    10741 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
    10801 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
    10861 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
    10921 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
    10981 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
    11041 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
    11101 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
    11161 tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
    11221 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
    11281 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
    11341 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
    11401 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
    11461 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
    11521 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
    11581 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
    11641 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
    11701 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
    11761 tgacccgcaa gcccggtgcc tgactcgagg gaattaattc gagctcggta cctttaagac
    11821 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
    11881 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
    11941 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    12001 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    12061 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc a
//
