LOCUS Exported 10046 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA). ACCESSION . VERSION . KEYWORDS pUDP002 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10046) AUTHORS Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM, Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG TITLE Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid. JOURNAL FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 10.1093/femsyr/foy012. PUBMED 29438517 REFERENCE 2 (bases 1 to 10046) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10046 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 290..1318 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA KE" CDS 1897..1920 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 1924..1944 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1945..6045 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 6049..6069 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" promoter 6988..7654 /gene="S. cerevisiae TDH3" /label=GAP promoter /note="promoter for glyceraldehyde-3-phosphate dehydrogenase" primer_bind 7524..7547 /label=GPDpro-F /note="S. cerevisiae GPD promoter, forward primer" primer_bind complement(7716..7734) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" CDS complement(8168..9028) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8791..8810 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9029..9133) /gene="bla" /label=AmpR promoter rep_origin 9236..9824 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9725..9744 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat ttgcatccat 61 acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc agacctgcga 121 gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag 181 aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc cttttaaaat 241 cttgctagga tacagttctc acatcacatc cgaacataaa caaggatcca tgggtaaaaa 301 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtgtc 361 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg 421 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt 481 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt 541 tagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct 601 gcctgaaacc gaactgcccg ctgttctgca accggtcgcg gaggccatgg atgcgatcgc 661 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca 721 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca 781 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct 841 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa 901 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg 961 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga 1021 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg 1081 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt 1141 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac 1201 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga 1261 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaataact 1321 cgaggacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat 1381 tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat 1441 catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg 1501 aatgctggtc gctatactgg agctctcgag aaccctcacc tttcgagagg acgatgcccg 1561 tgtctaaatg attcgaccag cctaagaatg ttcaacaatc tataatcagt ccatagtcaa 1621 caagagccct caaagttgat atcgtttagt gatctccctc gagatgagcg gaggatggtt 1681 gctttttggg gagccctaag tggtacgtgc ggcgagcggg atgtttcccc attaggcaaa 1741 ggacgaccgg gtaacccctc gagaaaaaaa atttttcgcc gctaatctgg tgttatataa 1801 agctccccct gctcctggaa tttttttcct tgtcaactca caccggaaat caaggcattt 1861 cattctgagt agttcttaaa aacataatca acaatggatt ataaagatga cgatgacaaa 1921 cctccaaaaa agaagagaaa ggtcgataag aaatactcaa taggcttaga tatcggcaca 1981 aatagcgtcg gatgggcggt gatcactgat gaatataagg ttccgtctaa aaagttcaag 2041 gttctgggaa atacagaccg ccacagtatc aaaaaaaatc ttataggggc tcttttattt 2101 gacagtggag agacagcgga agcgactcgt ctcaaacgga cagctcgtag aaggtataca 2161 cgtcggaaga atcgtatttg ttatctacag gagatttttt caaatgagat ggcgaaagta 2221 gatgatagtt tctttcatcg acttgaagag tcttttttgg tggaagaaga caagaagcat 2281 gaacgtcatc ctatttttgg aaatatagta gatgaagttg cttatcatga gaaatatcca 2341 actatctatc atctgcgaaa aaaattggta gattctactt ataaagcgga tttgcgctta 2401 atctatttgg ccttagcgca tatgattaag tttcgtggtc attttttgat tgagggagat 2461 ttaaatcctg ataatagtga tgtggacaaa ctatttatcc agttggtaca aacctacaat 2521 caattatttg aagaaaaccc tattaacgca agtggagtag atgctaaagc gattctttct 2581 gcacgattga gtaaatcaag acgattagaa aatctcattg ctcagctccc cggtgagaag 2641 aaaaatggct tatttgggaa tctcattgct ttgtcattgg gtttgacccc taattttaaa 2701 tcaaattttg atttggcaga agatgctaaa ttacagcttt caaaagatac ttacgatgat 2761 gatttagata atttattggc gcaaattgga gatcaatatg ctgatttgtt tttggcagct 2821 aagaatttat cagatgctat tttactttca gatatcctaa gagtaaatac tgaaataact 2881 aaggctcccc tatcagcttc aatgattaaa cgctacgatg aacatcatca agacttgact 2941 cttttaaaag ctttagttcg acaacaactt ccagaaaagt ataaagaaat cttttttgat 3001 caatcaaaaa acggatatgc aggttatatt gatgggggag ctagccaaga agaattttat 3061 aaatttatca aaccaatttt agaaaaaatg gatggtactg aggaattatt ggtgaaacta 3121 aatcgtgaag atttgctgcg caagcaacgg acctttgaca acggctctat tacccatcaa 3181 attcacttgg gtgagctgca tgctattttg agaagacaag aagactttta tccattttta 3241 aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc gaattcctta ttatgttggt 3301 ccattggcgc gtggcaatag tcgttttgca tggatgactc ggaagtctga agaaacaatt 3361 accccatgga attttgaaga agttgtcgat aaaggtgctt cagctcaatc atttattgaa 3421 cgcatgacaa actttgataa aaatcttcca aatgaaaaag tactaccaaa acatagtttg 3481 ctttatgagt attttacggt ttataacgaa ttgacaaagg tcaaatatgt tactgaagga 3541 atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag ccattgttga tttactcttc 3601 aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag attatttcaa aaaaatagaa 3661 tgttttgata gtgttgaaat ttcaggagtt gaagatagat ttaatgcttc attaggtacc 3721 taccatgatt tgctaaaaat tattaaagat aaagattttt tggataatga agaaaatgaa 3781 gatatcttag aggatattgt tttaacattg accttatttg aagataggga gatgattgag 3841 gaaagactta aaacatatgc tcacctcttt gatgataagg tgatgaaaca gcttaaacgt 3901 cgccgttata ctggttgggg acgtttgtct cgaaaattga ttaatggtat tagggataag 3961 caatctggca aaacaatatt agattttttg aaatcagatg gttttgccaa tcgcaatttt 4021 atgcagctga tccatgatga tagtttgaca tttaaagaag acattcaaaa agcacaagtg 4081 tctggacaag gcgatagttt acatgaacat attgcaaatt tagctggtag ccctgctatt 4141 aaaaaaggta ttttacagac tgtaaaagtt gttgatgaat tggtcaaagt aatggggcgg 4201 cataagccag aaaatatcgt tattgaaatg gcacgtgaaa atcagacaac tcaaaagggc 4261 cagaaaaatt cgcgagagcg tatgaaacga atcgaagaag gtatcaaaga attaggaagt 4321 cagattctta aagagcatcc tgttgaaaat actcaattgc aaaatgaaaa gctctatctc 4381 tattatctcc aaaatggaag agacatgtat gtggaccaag aattagatat taatcgttta 4441 agtgattatg atgtcgatca cattgttcca caaagtttcc ttaaagacga ttcaatagac 4501 aataaggtct taacgcgttc tgataaaaat cgtggtaaat cggataacgt tccaagtgaa 4561 gaagtagtca aaaagatgaa aaactattgg agacaacttc taaacgccaa gttaatcact 4621 caacgtaagt ttgataattt aacgaaagct gaacgtggag gtttgagtga acttgataaa 4681 gctggtttta tcaaacgcca attggttgaa actcgccaaa tcactaagca tgtggcacaa 4741 attttggata gtcgcatgaa tactaaatac gatgaaaatg ataaacttat tcgagaggtt 4801 aaagtgatta ccttaaaatc taaattagtt tctgacttcc gaaaagattt ccaattctat 4861 aaagtacgtg agattaacaa ttaccatcat gcccatgatg cgtatctaaa tgccgtcgtt 4921 ggaactgctt tgattaagaa atatccaaaa cttgaatcgg agtttgtcta tggtgattat 4981 aaagtttatg atgttcgtaa aatgattgct aagtctgagc aagaaatagg caaagcaacc 5041 gcaaaatatt tcttttactc taatatcatg aacttcttca aaacagaaat tacacttgca 5101 aatggagaga ttcgcaaacg ccctctaatc gaaactaatg gggaaactgg agaaattgtc 5161 tgggataaag ggcgagattt tgccacagtg cgcaaagtat tgtccatgcc ccaagtcaat 5221 attgtcaaga aaacagaagt acagacaggc ggattctcca aggagtcaat tttaccaaaa 5281 agaaattcgg acaagcttat tgctcgtaaa aaagactggg atccaaaaaa atatggtggt 5341 tttgatagtc caacggtagc ttattcagtc ctagtggttg ctaaggtgga aaaagggaaa 5401 tcgaagaagt taaaatccgt taaagagtta ctagggatca caattatgga aagaagttcc 5461 tttgaaaaaa atccgattga ctttttagaa gctaaaggat ataaggaagt taaaaaagac 5521 ttaatcatta aactacctaa atatagtctt tttgagttag aaaacggtcg taaacggatg 5581 ctggctagtg ccggagaatt acaaaaagga aatgagctgg ctctgccaag caaatatgtg 5641 aattttttat atttagctag tcattatgaa aagttgaagg gtagtccaga agataacgaa 5701 caaaaacaat tgtttgtgga gcagcataag cattatttag atgagattat tgagcaaatc 5761 agtgaatttt ctaagcgtgt tattttagca gatgccaatt tagataaagt tcttagtgca 5821 tataacaaac atagagacaa accaatacgt gaacaagcag aaaatattat tcatttattt 5881 acgttgacga atcttggagc tcccgctgct tttaaatatt ttgatacaac aattgatcgt 5941 aaacgatata cgtctacaaa agaagtttta gatgccactc ttatccatca atccatcact 6001 ggtctttatg aaacacgcat tgatttgagt cagctaggag gtgaccctcc aaaaaagaag 6061 agaaaggtct gagcggtttt gtataactaa ataatattgg aaactaaata cgaataccca 6121 aattttttat ctaaattttg ccgaaagatt aaaatctgca gagatatccg aaacaggtaa 6181 atggatgttt caatccctgt agtcagtcag gaacccatat tatattacag tattagtcgc 6241 cgcttaggca cgcctttaat tagcaaaatc aaaccttaag tgcatatgcc gtataaggga 6301 aactcaaaga actggcatcg caaaaatgaa aacatacgtt gaaactacgg caaaggattg 6361 gtcagatcgc ttcatacagg gaaagttcgg catcaacatc tttggataat atcagaatga 6421 gaaagaacag atacgcagta cgttttttgg tgagctcttt gcacttcttt agttctttcc 6481 atcaatatca gttttttaaa cttttaggac taaaagtgat gtttaacttc aaaatgttta 6541 aaattttgtt cttcccgacg ttcattaaga atactaatac actttaataa ttattttaat 6601 attttgttct aaataatgac ttttaattaa aaaagataaa atataaaaac atcataataa 6661 ctcaccagag gttaagaaca aaaaaacaaa ttagatatct gctaatccaa tatagttaaa 6721 tcaatctttc cttggtataa tgggtatatt acatatattt caaggaccga cactcctacc 6781 aaatatctaa aatttaccat attaacataa catgtatata aacgtcaaat cataatcagc 6841 actaacgtct cacggatcgt atatgccgta gcgacaatct aagaactatg cgaggacacg 6901 ctagggccaa ggcaaaaaga ttccttgatt acgtaaggga gttagaatca ttttgaataa 6961 aaaacacgct ttttcagttc gagtttatca ttatcaatac tgccatttca aagaatacgt 7021 aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag ccttttaatt 7081 ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat ataacatcgt 7141 aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag cccgcttttt 7201 aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc ttcaccaacc 7261 atcagttcat aggtccattc tcttagcgca actacagaga acaggggcac aaacaggcaa 7321 aaaacgggca caacctcaat ggagtgatgc aacctgcctg gagtaaatga tgacacaagg 7381 caattgaccc acgcatgtat ctatctcatt ttcttacacc ttctattacc ttctgctctc 7441 tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt tccctgaaat tattccccta 7501 cttgactaat aagtatataa agacggtagg tattgattgt aattctgtaa atctatttct 7561 taaacttctt aaattctact tttatagtta gtcttttttt tagttttaaa acaccaagaa 7621 cttagtttcg aataaacaca cataaacaaa caaacgagac ctgcggaggt ctccacaggc 7681 cccttttcct ttgtcgatat catgtaatta gttatgtcac gcttacattc acgccctccc 7741 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt 7801 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt 7861 tttctgtaca aacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt 7921 ttgggacaag cttacgtctc acggatcgta tatgccgtag cgacaatcta agaactatgc 7981 gaggacacgc tagcagctgt attcacgtag acggataggt atagccagac atcagcagca 8041 tacttcggga accgtaggcc accgagcggc cgcgattatc aaaaaggatc ttcacctaga 8101 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 8161 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 8221 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 8281 ctggccccag tgctgcaatg ataccgcggg acccacgctc accggctcca gatttatcag 8341 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 8401 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 8461 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 8521 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 8581 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 8641 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 8701 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 8761 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 8821 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 8881 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 8941 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 9001 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 9061 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 9121 taggggttcc gcgcacattt ccccgaaaag tgccacctgt catgaccaaa atcccttaac 9181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 9241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 9301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 9361 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 9421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 9481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 9541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 9601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 9661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 9721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 9781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 9841 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 9901 cccctgattc tgtggataac cgtgcggact atatgtgaag gcatggctat ggcacggcag 9961 acattccgcc agatcatcaa taggcacgac atggaggccc agaataccct ccttgacagt 10021 cttgacgtgc gcagctcagg ggcatg //