LOCUS Exported 9907 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION drives expression of V5-NES-APEX2-GFP from human PGK promotor. ACCESSION . VERSION . KEYWORDS hPGK_V5-APEX2-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9907) AUTHORS Markmiller S, Soltanieh S, Server KL, Mak R, Jin W, Fang MY, Luo EC, Krach F, Yang D, Sen A, Fulzele A, Wozniak JM, Gonzalez DJ, Kankel MW, Gao FB, Bennett EJ, Lecuyer E, Yeo GW TITLE Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. JOURNAL Cell. 2018 Jan 25;172(3):590-604.e13. doi: 10.1016/j.cell.2017.12.032. PUBMED 29373831 REFERENCE 2 (bases 1 to 9907) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9907 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 363..385 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 377..394 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 378..394 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 402..902 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 821..839 /label=hPGK-F /note="Human PGK promoter, forward primer" CDS 912..953 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 954..1700 /codon_start=1 /product="soybean ascorbate peroxidase, engineered to serve as a monomeric tag for electron microscopy and live-cell proteomics (Lam et al., 2015)" /label=APEX2 /note="more active than the original APEX due to the A134P mutation" /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK YAADEDAFFADYAEAHQKLSELGFADA" CDS 1707..1733 /codon_start=1 /product="nuclear export signal from the HIV Rev protein (Fischer et al., 1995)" /label=NES /translation="LPPLERLTL" CDS 1791..2453 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /label=CopGFP /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" misc_feature 2544..3132 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(3015..3026) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3206..3439 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 3511..3632 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind 3719..3752 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter 4106..4317 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" LTR 4330..4598 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" polyA_signal 5355..5476 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind 5976..6009 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter 6067..6566 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(6102..6125) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 6499..6518 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 6593..7165 /codon_start=1 /gene="Corynebacterium diphtheriae tox" /product="diphtheria toxin fragment A" /label=DTA /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI NFETRGKRGQDAMYEYMAQACAGNRVRR" intron 7242..7307 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" primer_bind complement(7375..7392) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7381..7605 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(7686..7702) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7686..7702) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7699..7721) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7710..7726 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7734..7764) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7779..7800 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7917..7934) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8088..8676) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8168..8187) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8847..9707) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9470..9489 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9708..9812) /gene="bla" /label=AmpR promoter primer_bind 9880..9898 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta cgccagggtt 361 ttcccagtca cgacgttgta aaacgacggc cagtgttggg gttgcgcctt ttccaaggca 421 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg 481 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg 541 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct 601 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg 661 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata 721 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg 781 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc 841 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc 901 aggaattcat gggtaagcct atccctaacc ctctcctcgg tctcgattct acgggaaagt 961 cttacccaac tgtgagtgct gattaccagg acgccgttga gaaggcgaag aagaagctca 1021 gaggcttcat cgctgagaag agatgcgctc ctctaatgct ccgtttggca ttccactctg 1081 ctggaacctt tgacaagggc acgaagaccg gtggaccctt cggaaccatc aagcaccctg 1141 ccgaactggc tcacagcgct aacaacggtc ttgacatcgc tgttaggctt ttggagccac 1201 tcaaggcgga gttccctatt ttgagctacg ccgatttcta ccagttggct ggcgttgttg 1261 ccgttgaggt cacgggtgga cctaaggttc cattccaccc tggaagagag gacaagcctg 1321 agccaccacc agagggtcgc ttgcccgatc ccactaaggg ttctgaccat ttgagagatg 1381 tgtttggcaa agctatgggg cttactgacc aagatatcgt tgctctatct gggggtcaca 1441 ctattggagc tgcacacaag gagcgttctg gatttgaggg tccctggacc tctaatcctc 1501 ttattttcga caactcatac ttcacggagt tgttgagtgg tgagaaggaa ggtctccttc 1561 agctaccttc tgacaaggct cttttgtctg accctgtatt ccgccctctc gttgacaaat 1621 atgcagcgga cgaagatgcc ttctttgctg attacgctga ggctcaccaa aagctttccg 1681 agcttgggtt tgctgatgcc ctgcagctgc ctcccctgga gcgcctgacc ctggacggtg 1741 gcggtggctc gggcggtggt gggtcgatgg agagcgacga gagcggcctg cccgccatgg 1801 agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg 1861 gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc 1921 tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca 1981 cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca 2041 acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct 2101 acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca 2161 gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca 2221 tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct 2281 actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc 2341 tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg 2401 agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc 2461 gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc 2521 gctagagctg aatctaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga 2581 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 2641 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 2701 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 2761 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 2821 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 2881 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 2941 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 3001 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 3061 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 3121 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 3181 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat 3241 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 3301 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 3361 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 3421 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 3481 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 3541 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 3601 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 3661 taactaaaat tcgagctcgg tacctcgcga atgcatcaga tctttccact caaaacatat 3721 aacttcgtat agcatacatt atacgaagtt atattcaaaa atctagacag cggccgccac 3781 tcaaaatttc ttctataaag taacaaaact tttatgaggg acagcccccc cccaaagccc 3841 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc 3901 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc 3961 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg 4021 cagacacctg gggggatacg gggaaaaggc ctccaaggcc ttaattcggc cgcaaggatc 4081 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 4141 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 4201 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 4261 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 4321 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 4381 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 4441 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 4501 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 4561 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 4621 atatcgccac catgacggag tataagccga cagttaggct tgctactagg gacgatgtgc 4681 cccgcgccgt ccgcacactg gcagcagctt ttgctgacta ccctgcaaca cggcacacgg 4741 tggacccgga caggcatatc gagagggtaa cagagctgca ggagctgttc ttgacgcggg 4801 taggtctgga tatcggtaag gtttgggtgg cagacgacgg agcagctgtc gcggtgtgga 4861 ccaccccgga gagcgttgag gcgggagcgg ttttcgccga aatcgggcct aggatggctg 4921 agttgtctgg gtccagactg gccgctcaac agcaaatgga gggcttgctg gcaccgcatc 4981 gcccgaaaga gccagcttgg ttcctggcaa ccgtgggcgt ttcacccgat caccaaggca 5041 agggtctggg aagtgcagtg gtgctgcccg gagtggaagc cgcagaacgc gcgggggtgc 5101 ccgcatttct ggagacaagt gcccctagga acctgccctt ctatgagaga cttggtttca 5161 ccgttacagc tgacgtggag gtgcccgagg gtccccgcac gtggtgcatg actcggaaac 5221 cgggcgcttg aaatcaacct ctggacgaaa ttccgcggtt attacaaaat ttgtgaaaga 5281 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 5341 cctttgtatc agttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 5401 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 5461 tcatcaatgt atcttatcat gtctggaatt gactcaaatg atgtcaatta gtctatcaga 5521 agctatctgg tctcccttcc gggggacaag acatccctgt ttaatattta aacagcagtg 5581 ttcccaaact gggttcttat atcccttgct ctggtcaacc aggttgcagg gtttcctgtc 5641 ctcacaggaa cgaagtccct aaagaaacag tggcagccag gtttagcccc ggaattgact 5701 ggattccttt tttagggccc attggtatgg ctttttcccc gtatcccccc aggtgtctgc 5761 aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg 5821 cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg 5881 gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg 5941 ggctttgggg gggggctgtc cctgatatca aacatataac ttcgtatagc atacattata 6001 cgaagttatt ttatcacgta agtagaacat gaaataacct agatcggatc cccgtcgact 6061 gcatgcgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc 6121 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg 6181 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc 6241 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag 6301 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag 6361 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg 6421 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa 6481 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct 6541 cctcttcctc atctccgggc ctttcgacct gcaggtcctc gccatggatc ctgatgatgt 6601 tgttgattct tctaaatctt ttgtgatgga aaacttttct tcgtaccacg ggactaaacc 6661 tggttatgta gattccattc aaaaaggtat acaaaagcca aaatctggta cacaaggaaa 6721 ttatgacgat gattggaaag ggttttatag taccgacaat aaatacgacg ctgcgggata 6781 ctctgtagat aatgaaaacc cgctctctgg aaaagctgga ggcgtggtca aagtgacgta 6841 tccaggactg acgaaggttc tcgcactaaa agtggataat gccgaaacta ttaagaaaga 6901 gttaggttta agtctcactg aaccgttgat ggagcaagtc ggaacggaag agtttatcaa 6961 aaggttcggt gatggtgctt cgcgtgtagt gctcagcctt cccttcgctg aggggagttc 7021 tagcgttgaa tatattaata actgggaaca ggcgaaagcg ttaagcgtag aacttgagat 7081 taattttgaa acccgtggaa aacgtggcca agatgcgatg tatgagtata tggctcaagc 7141 ctgtgcagga aatcgtgtca ggcgatctct ttgtgaagga accttacttc tgtggtgtga 7201 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa 7261 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg 7321 gaactgatga atgggagcag tggtggaatg cagatcctag agctcgctga tcagcctcga 7381 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 7441 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 7501 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 7561 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa 7621 gaaccagctg gggctcgaga tccactagtt ctagcctcga ggcatgcaag cttggcgtaa 7681 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 7741 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 7801 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 7861 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 7921 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 7981 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 8041 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 8101 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 8161 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 8221 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 8281 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 8341 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 8401 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 8461 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 8521 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 8581 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 8641 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 8701 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 8761 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 8821 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 8881 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 8941 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 9001 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 9061 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 9121 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 9181 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 9241 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 9301 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 9361 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 9421 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 9481 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 9541 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 9601 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 9661 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 9721 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 9781 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 9841 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 9901 tttcgtc //