LOCUS Exported 12248 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus. ACCESSION . VERSION . KEYWORDS HR_G3BP1-V5-APEX2-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12248) AUTHORS Markmiller S, Soltanieh S, Server KL, Mak R, Jin W, Fang MY, Luo EC, Krach F, Yang D, Sen A, Fulzele A, Wozniak JM, Gonzalez DJ, Kankel MW, Gao FB, Bennett EJ, Lecuyer E, Yeo GW TITLE Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. JOURNAL Cell. 2018 Jan 25;172(3):590-604.e13. doi: 10.1016/j.cell.2017.12.032. PUBMED 29373831 REFERENCE 2 (bases 1 to 12248) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12248 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 363..385 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 377..394 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 378..394 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS 1929..1970 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 1971..2717 /codon_start=1 /product="soybean ascorbate peroxidase, engineered to serve as a monomeric tag for electron microscopy and live-cell proteomics (Lam et al., 2015)" /label=APEX2 /note="more active than the original APEX due to the A134P mutation" /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK YAADEDAFFADYAEAHQKLSELGFADA" CDS 2724..2750 /codon_start=1 /product="nuclear export signal from the HIV Rev protein (Fischer et al., 1995)" /label=NES /translation="LPPLERLTL" CDS 2808..3470 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /label=CopGFP /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" misc_feature 3561..4149 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(4032..4043) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 4223..4456 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 4528..4649 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind 4736..4769 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter 5123..5334 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" LTR 5347..5615 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" polyA_signal 6372..6493 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind 6993..7026 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter 8408..8907 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(8443..8466) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 8840..8859 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 8934..9506 /codon_start=1 /gene="Corynebacterium diphtheriae tox" /product="diphtheria toxin fragment A" /label=DTA /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI NFETRGKRGQDAMYEYMAQACAGNRVRR" intron 9583..9648 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" primer_bind complement(9716..9733) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 9722..9946 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(10027..10043) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10027..10043) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10040..10062) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10051..10067 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10075..10105) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10120..10141 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10258..10275) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10429..11017) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10509..10528) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11188..12048) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11811..11830 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12049..12153) /gene="bla" /label=AmpR promoter primer_bind 12221..12239 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta cgccagggtt 361 ttcccagtca cgacgttgta aaacgacggc cagtgtcccc ggcccttagt ttgctagtcc 421 ttaaagtagg tatctaccat cagagggttg ttgtaaaggt taagtgagct aataaatttg 481 aatagagctg ggcacagtgg ctcacgcctg taatcccagc actttgggag gccgaggcgg 541 gcggatcacg aggtcaggag attgagacca tcctggctaa cacggtgaaa ccccgtctct 601 actaaaaata caaaaaatta gctgggtgtg gtggcggacg cctgtagtcc cagctactcg 661 ggagactgag gcaggagaat ggcgtgaatc tgggaggcgg agcttgcagt gagccgagat 721 cgcgccactg cactccagcc tgggcagcag agcgagactc catctcaaaa aataaataaa 781 taaataaatt tgaatagaac acttccgcga cacatagtaa gtactcttag atactcattt 841 taagaaatta gaaaattaca ttgctggtga agagcataaa ttgaatatat tgaaatgttt 901 cgtagcccct taactacttt ggctctgtta ggaggaaacg aaggccaaga ctatccaaaa 961 ttctcaggag gtgttaaaga gcaaattaaa gtttatgtca aattaatgaa gtttttattt 1021 taaatgtata tgtactttgc ataaatatgt acagtaccat cttttcacac tggtgatgat 1081 gaggttttat tctaagcctc caaatctatg gagaaggtgg caattttttt tattttttat 1141 tttttattgg gacggagtct cactctgtcg cccatgctgg agtgcagtgg cacgaccttg 1201 gctcaccaca acttccgcca cccgggttca agttgcactc cagtctgggt gacgagtgaa 1261 actctgtctc attaaaaaaa caaacaaaaa actgaactgt gagaacttag ctgatttata 1321 atctgctggt tctttttttt ttctgagtcg gagtctcgct ctttttccca gactggagta 1381 caatggcgtg atcttggctc actgcaacct ccgcctcctg ggttcaagca attctcctgc 1441 ctcagcctcc ttgaatagct ggaactacag gcgtgtgtca ccatgtctgg ctaatttttg 1501 tgttttttta gtagagacgg ggtttcacca tgttggtcag gctggtcttg aactcctgac 1561 ctcgtaatcc acccgtctcg gcctcccaaa gtgctgggat tacaggcatg agtcaccgtg 1621 cccagcctct gctggttcat tattacagct ttctttatct ttgatagtga tagccagctc 1681 atcagtactc ttaagtctgg tcaccttgat tcttacagcc catcatgttc agaggtgagg 1741 tccgtctgaa tgtcgaagag aagaagactc gagctgccag ggaaggcgac cgacgagata 1801 atcgccttcg gggacctgga ggccctcgag gtgggctggg tggtggaatg agaggccctc 1861 cccgtggagg catggtgcag aaaccaggat ttggagtggg aagggggctt gcgccacggc 1921 aggaattcgg taagcctatc cctaaccctc tcctcggtct cgattctacg ggaaagtctt 1981 acccaactgt gagtgctgat taccaggacg ccgttgagaa ggcgaagaag aagctcagag 2041 gcttcatcgc tgagaagaga tgcgctcctc taatgctccg tttggcattc cactctgctg 2101 gaacctttga caagggcacg aagaccggtg gacccttcgg aaccatcaag caccctgccg 2161 aactggctca cagcgctaac aacggtcttg acatcgctgt taggcttttg gagccactca 2221 aggcggagtt ccctattttg agctacgccg atttctacca gttggctggc gttgttgccg 2281 ttgaggtcac gggtggacct aaggttccat tccaccctgg aagagaggac aagcctgagc 2341 caccaccaga gggtcgcttg cccgatccca ctaagggttc tgaccatttg agagatgtgt 2401 ttggcaaagc tatggggctt actgaccaag atatcgttgc tctatctggg ggtcacacta 2461 ttggagctgc acacaaggag cgttctggat ttgagggtcc ctggacctct aatcctctta 2521 ttttcgacaa ctcatacttc acggagttgt tgagtggtga gaaggaaggt ctccttcagc 2581 taccttctga caaggctctt ttgtctgacc ctgtattccg ccctctcgtt gacaaatatg 2641 cagcggacga agatgccttc tttgctgatt acgctgaggc tcaccaaaag ctttccgagc 2701 ttgggtttgc tgatgccctg cagctgcctc ccctggagcg cctgaccctg gacggtggcg 2761 gtggctcggg cggtggtggg tcgatggaga gcgacgagag cggcctgccc gccatggaga 2821 tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag 2881 agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga 2941 ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct 3001 accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca 3061 cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg 3121 aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg 3181 tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg 3241 gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact 3301 acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc 3361 agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc 3421 tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc agatcccgcg 3481 ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgct 3541 agagctgaat ctaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg 3601 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 3661 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 3721 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 3781 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 3841 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 3901 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat 3961 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 4021 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 4081 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 4141 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 4201 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg 4261 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 4321 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 4381 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 4441 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 4501 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 4561 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 4621 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa 4681 ctaaaattcg agctcggtac ctcgcgaatg catcagatct ttccactcaa aacatataac 4741 ttcgtatagc atacattata cgaagttata ttcaaaaatc tagacagcgg ccgccactca 4801 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca 4861 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct 4921 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg 4981 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag 5041 acacctgggg ggatacgggg aaaaggcctc caaggcctta attcggccgc aaggatctgc 5101 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 5161 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa 5221 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 5281 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag 5341 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca 5401 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc 5461 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc 5521 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc 5581 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgata 5641 tcgccaccat gacggagtat aagccgacag ttaggcttgc tactagggac gatgtgcccc 5701 gcgccgtccg cacactggca gcagcttttg ctgactaccc tgcaacacgg cacacggtgg 5761 acccggacag gcatatcgag agggtaacag agctgcagga gctgttcttg acgcgggtag 5821 gtctggatat cggtaaggtt tgggtggcag acgacggagc agctgtcgcg gtgtggacca 5881 ccccggagag cgttgaggcg ggagcggttt tcgccgaaat cgggcctagg atggctgagt 5941 tgtctgggtc cagactggcc gctcaacagc aaatggaggg cttgctggca ccgcatcgcc 6001 cgaaagagcc agcttggttc ctggcaaccg tgggcgtttc acccgatcac caaggcaagg 6061 gtctgggaag tgcagtggtg ctgcccggag tggaagccgc agaacgcgcg ggggtgcccg 6121 catttctgga gacaagtgcc cctaggaacc tgcccttcta tgagagactt ggtttcaccg 6181 ttacagctga cgtggaggtg cccgagggtc cccgcacgtg gtgcatgact cggaaaccgg 6241 gcgcttgaaa tcaacctctg gacgaaattc cgcggttatt acaaaatttg tgaaagattg 6301 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 6361 ttgtatcagt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 6421 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 6481 tcaatgtatc ttatcatgtc tggaattgac tcaaatgatg tcaattagtc tatcagaagc 6541 tatctggtct cccttccggg ggacaagaca tccctgttta atatttaaac agcagtgttc 6601 ccaaactggg ttcttatatc ccttgctctg gtcaaccagg ttgcagggtt tcctgtcctc 6661 acaggaacga agtccctaaa gaaacagtgg cagccaggtt tagccccgga attgactgga 6721 ttcctttttt agggcccatt ggtatggctt tttccccgta tccccccagg tgtctgcagg 6781 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc 6841 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag 6901 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc 6961 tttggggggg ggctgtccct gatatcaaac atataacttc gtatagcata cattatacga 7021 agttatttta tcacgtaagt agaacatgaa ataacctaga tcgatcttca tggatcttca 7081 tgcagccata caaaccctgg ttccaacaga atggtgaatt ttcgacagcc tttggtatct 7141 tggagtatga ccccagtctg ttataaactg cttaagtttg tataatttta ctttttttgt 7201 gtgttaatgg tgtgtgctcc ctctccctct cttccctttc ctgaccttta gtctttcact 7261 tccaattttg tggaatgata ttttaggaat aacggacttt taaagaagca aaaaaaaaga 7321 ctgaatttcc ttgcttactt tgcatataca gactggattt tttttttttt tttacagcca 7381 tttccccaaa ggaatgtctt gcatattact gacatttggt atgtttcatt cattggaata 7441 tttcttattt tctacgtgtt tgaaaagcct gtaagaaata caggatttga taatattttg 7501 aaggcaggaa aaacccaaat tgtttcttct ttgagagtca tgactacctt ctggtgtgga 7561 gaaattgcca ttggaaaatt tgacaatttt gattctcact ggtatgttta aaaactgaat 7621 aaaaggaata gaattttttt ttgataaagg atcacaaaac aattctaaaa cctaactgtt 7681 tttaccattg aaatttaaat tgtgataata ggttttaaat gtctagaatg caactgatag 7741 gcttttcttg aactgttagt ttttttgaag tagttttttc atgtttaatt tgtatttgta 7801 aaaaaacaaa aagcaaaaaa attcccaaaa cccagataac aaccagagca aaactgttgt 7861 gccttctatt tatctttgat ttcagtcttg gcaattgttt aaaaaaaaaa tctagatttg 7921 ttttattagg ttcagagtat gtggggaatt atagaatccc tctttcatca ctttgtgtat 7981 gtcttttgtt aacatatttg ttatgcctta ttctaaaatt gagtctcaaa ctggaatgcc 8041 tttgaagaca gatgcttcta tagaggttct ttgacctaaa tagttcagca tttgtatttt 8101 tattctggta tctaatcaga ttcctaatca tagcccgtaa gaaggaatgt tactttaata 8161 ttggactttg ctcatgtgct cgtgtccgca tttttttttt tcttaaaatc atagccatat 8221 ggtaaatttt ctattttgtt atggttctct tttattgatg ggcatgcagt gggtgttact 8281 tggaaatggc caatttttat taaaatattt ctggaagaaa atttaatctg gcaatataga 8341 ctaatactcg tttcacatat gtgttatttg ttgagtgctg tgtgtgagat gggtgaaggc 8401 tgcatgcggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc 8461 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc 8521 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc 8581 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa 8641 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta 8701 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg 8761 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga 8821 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc 8881 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg 8941 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac 9001 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa 9061 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat 9121 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt 9181 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag 9241 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca 9301 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt 9361 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga 9421 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag 9481 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg 9541 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta 9601 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat 9661 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg 9721 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 9781 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 9841 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 9901 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 9961 agaaccagct ggggctcgag atccactagt tctagcctcg aggcatgcaa gcttggcgta 10021 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 10081 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 10141 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 10201 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 10261 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 10321 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 10381 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 10441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 10501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 10561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 10621 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 10681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 10741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 10801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 10861 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 10921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 10981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 11041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 11101 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 11161 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 11221 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 11281 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 11341 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 11401 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 11461 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 11521 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 11581 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 11641 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 11701 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 11761 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 11821 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 11881 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 11941 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 12001 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 12061 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 12121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 12181 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 12241 ctttcgtc //