LOCUS Exported 1353 bp ds-DNA linear SYN 04-JUN-2021 DEFINITION AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression. ACCESSION . VERSION . KEYWORDS pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1353) AUTHORS Dana H, Sun Y, Mohar B, Hulse BK, Kerlin AM, Hasseman JP, Tsegaye G, Tsang A, Wong A, Patel R, Macklin JJ, Chen Y, Konnerth A, Jayaraman V, Looger LL, Schreiter ER, Svoboda K, Kim DS TITLE High-performance calcium sensors for imaging activity in neuronal populations and microcompartments. JOURNAL Nat Methods. 2019 Jul;16(7):649-657. doi: 10.1038/s41592-019-0435-6. Epub 2019 Jun 17. PUBMED 31209382 REFERENCE 2 (bases 1 to 1353) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 4, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1353 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 10..27 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 31..63 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 67..90 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" regulatory 97..106 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 118..174 /codon_start=1 /product="calmodulin-binding peptide from avian smooth muscle myosin light chain kinase" /label=calmodulin-binding peptide /translation="RRKWNKTGHAVRAIGRLSS" primer_bind 387..408 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(514..535) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(775..794) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" ORIGIN 1 atgggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg 61 ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt 121 cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag ctcacagcct 181 aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt caagatccgc 241 cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc 301 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc caaactttcg 361 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 421 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag 481 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 541 ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc 601 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 661 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 721 ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac 781 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 841 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 901 aaccttcctg accaactgac tgaagagcag atcgcagaat ttaaagaggc tttctcccta 961 tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcgttct 1021 ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac 1081 ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa agggagctac 1141 agggacagtg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga tggcaatggc 1201 tacatcagtg cagcagagct tcgccacgtg atgaacaacc ttggagagaa gttaacagat 1261 gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac 1321 gaagagtttg tacaaatgat gacagcgaag tga //