LOCUS Exported 2208 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION ODPAMTS in domestication vector. ACCESSION . VERSION . KEYWORDS pUPD2-ODPAMTS (12) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2208) AUTHORS Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, Navarro-Rodriguez M, Rubio LM, Caro E TITLE Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria. JOURNAL BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 10.1186/s12896-017-0393-y. PUBMED 29132331 REFERENCE 2 (bases 1 to 2208) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2208 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..44 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 47..68 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" terminator 228..285 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(309..326) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(480..1068) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(560..579) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1251..1345 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1366..2025) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1940..1959 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 2187..2205 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc 61 ttctagagcg atgagtggtc tcgccatgct tgctgcttca ttcaaacgcc aaccatcaca 121 attggtccgc gggttaggag ctgttcttcg cactcccacc aggataggtc atgttcgtac 181 catgtcaatg tgagaccact catcgcgact agtagcggcc gctgcagtcc ggcaaaaaag 241 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg 301 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 361 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 421 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 481 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 541 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 601 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 661 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 721 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 781 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 841 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 901 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 961 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 1021 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 1081 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 1141 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 1201 tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca 1261 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc 1321 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg 1381 ccactcgtcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca 1441 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata 1501 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa 1561 actggtgaaa ctcacccagg gattggctga tacgaaaaac atattctcaa taaacccttt 1621 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa 1681 ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa aacgtttcag tttgctcatg 1741 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc 1801 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata 1861 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt 1921 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca 1981 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc 2041 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg 2101 aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca 2161 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcaga //