LOCUS Exported 1263 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7. ACCESSION . VERSION . KEYWORDS pET28a-HaloTag7-SnoopLigase SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1263) AUTHORS Buldun CM, Jean JX, Bedford MR, Howarth M TITLE SnoopLigase Catalyzes Peptide-Peptide Locking and Enables Solid-Phase Conjugate Isolation. JOURNAL J Am Chem Soc. 2018 Feb 28;140(8):3008-3018. doi: 10.1021/jacs.7b13237. Epub 2018 Feb 14. PUBMED 29402082 REFERENCE 2 (bases 1 to 1263) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1263 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 13..30 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 40..924 /codon_start=1 /product="modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives" /label=HaloTag(R) /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG SEIARWLSTLEISG" ORIGIN 1 atgggcagca gccatcatca tcatcatcac agcagcggcg aaatcggtac tggctttcca 61 ttcgaccccc attatgtgga agtcctgggc gagcgcatgc actacgtcga tgttggtccg 121 cgcgatggca cccctgtgct gttcctgcac ggtaacccga cctcctccta cgtgtggcgc 181 aacatcatcc cgcatgttgc accgacccat cgctgcattg ctccagacct gatcggtatg 241 ggcaaatccg acaaaccaga cctgggttat ttcttcgacg accacgtccg cttcatggat 301 gccttcatcg aagccctggg tctggaagag gtcgtcctgg tcattcacga ctggggctcc 361 gctctgggtt tccactgggc caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg 421 gagttcatcc gccctatccc gacctgggac gaatggccag aatttgcccg cgagaccttc 481 caggccttcc gcaccaccga cgtcggccgc aagctgatca tcgatcagaa cgtttttatc 541 gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg aagtcgagat ggaccattac 601 cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt ggcgcttccc aaacgagctg 661 ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg aagaatacat ggactggctg 721 caccagtccc ctgtcccgaa gctgctgttc tggggcaccc caggcgttct gatcccaccg 781 gccgaagccg ctcgcctggc caaaagcctg cctaactgca aggctgtgga catcggcccg 841 ggtctgaatc tgctgcaaga agacaacccg gacctgatcg gcagcgagat cgcgcgctgg 901 ctgtctactc tggagatttc cggtggtagc ggtgggtctg gtagtggtgt gaataagaac 961 gataaaaagc cgctgcgtgg tgccgtgttt agcctgcaga aacagcatcc cgactatccc 1021 gatatctatg gcgcgattga tcagaatggg acctatcaaa atgtgcgtac cggcgaagat 1081 ggtaaactga cctttaagaa tctgagcgat ggcaaatatc gcctgtttga aaatagcgaa 1141 cccccgggct ataaaccggt gcagaataag ccgattgtgg cgtttcagat tgtgaatggc 1201 gaagtgcgtg atgtgaccag cattgtgccg ccgggtgtgc cggctacata tgaatttacc 1261 taa //