LOCUS       Exported                4131 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses human IFITM3-delta1-21 with an N-terminal myc tag in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    myc-hIFITM3-delta1-21
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Chesarino NM, McMichael TM, Yount JS
  TITLE     E3 Ubiquitin Ligase NEDD4 Promotes Influenza Virus Infection by 
            Decreasing Levels of the Antiviral Protein IFITM3.
  JOURNAL   PLoS Pathog. 2015 Aug 11;11(8):e1005095. doi: 
            10.1371/journal.ppat.1005095. eCollection 2015 Aug.
  PUBMED    26263374
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     44..66
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     58..75
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     59..75
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        102..405
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        406..609
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     560..580
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     606..630
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          747..843
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    1345..1479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1369..1388)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1423..1442
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1590..1606)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1590..1606)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1603..1625)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1614..1630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1638..1668)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1683..1704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1821..1838)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1992..2580)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2072..2091)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2751..3611)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3374..3393
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3612..3716)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3784..3802
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3840..3862)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3962..3981
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
       61 aaaacgacgg ccagtgagtt cgagcttgca tgcctgcagg tcgttacata acttacggta
      121 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      181 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      241 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      301 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      361 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      421 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      481 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      541 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      601 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
      661 ctccatagaa gacaccggga ccgatccagc ctccggactc tagaggatcc ggtactagag
      721 gaactgaaaa accagaaagt taactggtaa gtttagtctt tttgtctttt atttcaggtc
      781 ccggatccgg tggtggtgca aatcaaagaa ctgctcctca gtggatgttg cctttacttc
      841 taggcctgta cggaagtgtt acttctgctc taaaagctgc ggaattgtac ccgcgggccc
      901 accatggcat caatgcagaa gctgatctca gaggaggacc tgcttatggc catggaggcc
      961 cgaattccca tgctcaagga ggagcacgag gtggctgtgc tgggggcgcc ccacaaccct
     1021 gctcccccga cgtccaccgt gatccacatc cgcagcgaga cctccgtgcc cgaccatgtc
     1081 gtctggtccc tgttcaacac cctcttcatg aacccctgct gcctgggctt catagcattc
     1141 gcctactccg tgaagtctag ggacaggaag atggttggcg acgtgaccgg ggcccaggcc
     1201 tatgcctcca ccgccaagtg cctgaacatc tgggccctga ttctgggcat cctcatgacc
     1261 attctgctca tcgtcatccc agtgctgatc ttccaggcct atggataggt cgaccgagat
     1321 ctctcgaggt accgcggccg cggggatcca gacatgataa gatacattga tgagtttgga
     1381 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     1441 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     1501 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt cggatcctct agagtcgatc
     1561 tgcaggcatg ctagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     1621 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     1681 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1741 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1801 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1861 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1921 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1981 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2041 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2101 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2161 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2221 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2281 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2341 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2401 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2461 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2521 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2581 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2641 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2701 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2761 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2821 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2881 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2941 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3001 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3061 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3121 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3181 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3241 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3301 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3361 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3421 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3481 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3541 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3601 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3661 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3721 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     3781 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     3841 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3901 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     3961 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     4021 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     4081 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg g
//
