LOCUS       Exported               13378 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Ubiquitously expresses a secreted and fluorescently tagged Annexin5 
            for in-vivo apoptosis reporter.
ACCESSION   .
VERSION     .
KEYWORDS    Tol2-BetaActin-SecA5-YFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13378)
  AUTHORS   Hsu AY, Gurol T, Sobreira TJP, Zhang S, Moore N, Cai C, Zhang ZY, 
            Deng Q
  TITLE     Development and Characterization of an Endotoxemia Model in Zebra 
            Fish.
  JOURNAL   Front Immunol. 2018 Mar 29;9:607. doi: 10.3389/fimmu.2018.00607. 
            eCollection 2018.
  PUBMED    29651289
REFERENCE   2  (bases 1 to 13378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13378
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(7..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1324..1344
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6651..6675
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     6677..6693
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6703..6721
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7757..7781)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      7795..7804
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7801..8520
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7846..7867)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8107..8126)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8454..8475
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(8557..8575)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(8604..8628)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8633..8767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8657..8676)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8711..8730
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(11141..11157)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11141..11157)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(11194..11212)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11233..11249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11233..11249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11246..11268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11257..11273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11281..11311)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11326..11347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11464..11481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11635..12223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11715..11734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12394..13254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13017..13036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13255..13359)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcaacttt gtatagaaaa gttgaaaatt ccagtttgaa gaaacttttc aagactcacc
     1381 ttttcactgt tggtcttaat ttaggttgat tatttttgtc aatttaataa ttacattgta
     1441 ttgttcatat tttattgtct tattgtggtg tttttacagc ttttttattg ttctgctttg
     1501 ttactgtttg ccttgtatgc attgtagcgc tttgggtctg agaaaagcgc actataaata
     1561 aactgtatta ttattattag ggatggacat cacggtggcg cgatgggtgg cacaattacc
     1621 tcacagcaag taggttgctg tttagagccc cggctggatc agttggcagt tctgtgtgga
     1681 gtttgcatgt tctccctgtt caagtaggtt tcctcccggt gctctggttt cccctcacaa
     1741 gtccaaagac atgatgtata ggtgaatggg gtaagctaaa ttgaccatag tgagtgtgtg
     1801 tgtgtgtgtg aatgagagtg tataggtgtt tcccagtgtt aggttgcaac tggaaggaca
     1861 tctgctgtgt aaaacatatg caggataagt tggcggttca ttcccctatg gtgacccctg
     1921 gttaattaaa ggactaagtt gaaaatgaat gaatgaatga atgattagag ataaaccgat
     1981 accacttttt tacaaaccga tatgagtaca agtataatag tttctgttac tcgacaatac
     2041 tgagtaccga ttctgatact tcttggattt ggtttcaatg taattaattt gaaagaaggt
     2101 tttatttctt gcctgtcgca aggatgaaca caaaaatctt taccagaagg atattttaag
     2161 ctaagaatat acgcacgttg ttgacaaccc tgtatcgatt accgattaaa cgtggacatt
     2221 taaaggataa gttatgcttg cgccatcaca actttacaca ttacatgatc aaagatcata
     2281 ttgaatattc ggtttattcg tcagaagcct atagatttgt taaaagtgta ttttataaac
     2341 ggatggttta taaagactaa gctattaata tatcagacat tcattaatac atcaagacat
     2401 cgatgtcagg taggacaaca aatcacatcc atgtaatgaa atcttgttga gtggtattgg
     2461 tgcgtggttt cagcatttca tgtgcaatac gagtaagaat attttaacca agtatcgacc
     2521 caaaacctga tactggtatc ggtgcatccc ttattattat tattatcaaa aacacacttg
     2581 ttttatttat gtgggtgcca aacaacaaac tatgaaatgc agcatgacac gcccaaaaag
     2641 tgttcctttg agaacaactt cataaatatg tttcttatca ttatgcatga atggtgtact
     2701 gggagagctt tggtatcaga attgagataa gagactttgg cattctaaag gacaatgaca
     2761 ttatcacact gacagtcaac aacactttat tgctctggaa atcttatgtc atggggcaaa
     2821 gctgaggttc attaggacag taccttattt agagatgaat gggaaggctg tagtgggctg
     2881 ctatcaggga agggtggtgg tcctcggggg aactatggac tcacaaaagc acatctgctg
     2941 taacaagatc ccctgtgcaa tctacaaaag ttacaaagag aagcatggtt ggttaaaaaa
     3001 tagaattgac tggatatgct gaatgcattg tttgttgtgt gttacatcac attaaccaca
     3061 gattggtgct aaatatttgc ttatcttata aaaacctgtg tgtttataaa ggggggtgtg
     3121 gatggatgct tggcattctt tgaaaagatg ttgggggatg gaaggaggag accttattga
     3181 tgagtatcca gtttgcagat aacaagtttt tagacattca tactatgatg ctgtttatgt
     3241 ctgttttttc tcattttttc tatacacaaa cagctataaa acattcactt ttatatctga
     3301 tgctgtatat taaatttcgt tgtatttatt ctgtttgctc tgttgggtca tagctagggc
     3361 cagacagaat ctgctgacat tttttgcttt ttctgcagag aattttgtaa acaatctgtg
     3421 atttatgcga gattttttcg ggagtatcgt aactaaaaac ttactatatg aaataaaaaa
     3481 aaataatacc tttttaactt ttaataaata tatacaatgc aagtccacct agatccaatt
     3541 atttggtcga caaagcaagt ctctcacata atatatcttc taaaagacag aaaacattac
     3601 tttcagacca tattgtaatc aaatgaacac tttcatatta gtcaataata ttacggaaat
     3661 gtatttaaaa actgaataaa catatattta cacctttgtc tttatttcta gagtgctttt
     3721 acaatgtaga ttgtgtcaaa gcagcttaac ttagaggatt agtatagtaa attgaaactg
     3781 tgtcagtcca ttttttaaga gtttaagttc agttcagttt agttcagtgt gagttaattt
     3841 ttactgctga gagtccaaac actaaagagc aaatccaaat acacaagtca ataaataaac
     3901 tcaatgatgg gctaaagatc tggggaaatc tgcagatttc tgtgcgcaca gattctgtgt
     3961 gggcctagtc attacctata ctcaacgttt actctgagaa acttccatat tttagaggta
     4021 aaatgctcag aattgtaaaa agtcttgtca aaatacttgg aatttgccaa aaacaaaata
     4081 aaagtcacat cacctcattg tccttggtat cagcaagcac ttttaacaca atcagctgtt
     4141 tttgtttata actcccactg aaggccatgt gcattactcc caagttacac agtaaaaagt
     4201 cacatgtagt ttatcatgat cactgctgct caagcacagc tttatctgtt attcagtgtg
     4261 gcaatatctg aaatgtgtgc aaaatattat ttattgtagc tgatcaccaa cactttcagt
     4321 gttttttttt tctactttta atgagaatta tgttcttagg atttatcctt ataaacctct
     4381 ttaaaaagaa atgttatgtc tgcatagaca cgacatgaaa ttatgaacag aaaatttaac
     4441 tatttaagat caagacttga catataacat ttctaatgaa cattaaacct gaggtaggcc
     4501 cacttgcaaa aatgtaactg ctatcactca ctttatacat cattgtgtaa cttgtacaca
     4561 caaaaatagt gaagcaatgt ggcatggaat gcaggtccca ttcattcaaa gttattaaag
     4621 cactttctta aagtgcatta ctactgagct aagaccagct agttgctcat acaacatact
     4681 acaagtagtg tcatcctaat ttggataaaa aaaatgtcca ttttatacat ggaaaagtac
     4741 tcattagatc ccccattgtt tgtattacgg tatttcgtga acacaagagg taaatgacct
     4801 acagagctgc tgttgtgtta gattgaaaac acaacacagg atcatggagg catccatcat
     4861 tatgacgcat tctgccactt gtaactctgc acagactttt tgaaaagttt aattgagtct
     4921 tggcgttgtc gcagaaaaat acgaaaaatt ctgtgttaaa atttgcaaat aaaaattcca
     4981 gttttatagg aaatcgccat ggccttgttc tttaaccagt taagcttccc cttctttcac
     5041 tctcaagttg caagaagcaa gtgtagcaat gtgcacgcga cagccgggtg tgtgacgctg
     5101 gaccaatcag agcacagagc tccgaaagtt taccttttat ggctagagcc gggtatgtgc
     5161 cgtcatataa aagagcgcgc ccagcttttc agcctcactt tgagctcctc cacacgcagc
     5221 tagtgcggaa tatcatctgc ttgtaaccca ttctcttaag tcgacaaccc cccaaaccca
     5281 aggtgagttg gttttacagt ttttaagcgt ttaattatag tttctatcta ttaatgttaa
     5341 gtaaaatgtg taaatggata aatatgattt gatgaagtct ttagacagtt cataacggtc
     5401 tctttgtagt tatctactag gtttgattta taagtggtgg ttcttttgat aatctatgtt
     5461 acatttaata aatattggct gtatcttaac ttttttgtag cgttagtgga tgtggcaggt
     5521 gagaatgtcg gtaatttaac gtgaccagtc gtaggcacga cattgaacat tgaataggcc
     5581 ggtgtgaaat gggtgttaag tcttaatata aagttgtgtt ctggctaatc tctctttaac
     5641 agccataaat gtgattttta actacagttt tgagggtgct ggtgagcatg ttgcacactt
     5701 gatggatagc cggcatggga agttctttgt gcaggcagtg ctgcagcagg gtgtgaccta
     5761 ctttagctag ccggctaacc agctctcatc tgatgtaacg taaaccccat caatctaaca
     5821 tcggctcatt cgtgctttat aagatcgaag atcttgttat tagtaacggg tattggcatt
     5881 tagttttaac ttggttaatt ttctttttgt tatgtgttct atttaaatgt agcctgcagc
     5941 cccctctgtc tttaatgaag ctgaagatta acgttaacgt tagtgaagtg ttgtatccgc
     6001 tatgaaagat ttgaatttta gaggtgtggt ccactttaat aacggctgcg gagttctctg
     6061 gctgcacttc agccgctgtg actgtcgaag cagagcccat cagtttttca ggatctcggt
     6121 gatttttgag gctagactat gaactgaacc gactgagagt gaccgtccag aaatgcttta
     6181 gtgtgaagtc tccgctcacg aggagtccgt ggactgactg cagatctgtg acgcagtaac
     6241 acccggcggc agacacccgt tgaaatgcgg ttgtgtaatt gatcgcaggc gagggtggaa
     6301 gaggatgtga aacttcattt gtgtaaaatt tagggagtgg tcctggtgtg atgaatgtcg
     6361 aaatctgttc ctttttactg agccctacga ctctggctga gtgccacacc gccggcagcc
     6421 gcaaagcggc tcaaaccatt gccttttatg gtaataatga gagaatgcag agggacttcc
     6481 tttgtctggc atatctgagg cgcgcgctgt cactagcgcc caccagcggt cagactgtag
     6541 aatgcagagc aaaacaggaa gttgactcca gatggtcaca tgcttgctga aacgcctctc
     6601 cctggcagca gtgcaattct aacagtttcc ctttctttta cagttcagcc tcaagtttgt
     6661 acaaaaaagc aggcttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     6721 ggcgaattgg gtaccgccac catgcataag gttttgctgg cactgttctt tatctttctg
     6781 gcaccagcag gcaccatggc acaggttctc agaggcactg tgactgactt ccctggattt
     6841 gatgagcggg ctgatgcaga aactcttcgg aaggctatga aaggcttggg cacagatgag
     6901 gagagcatcc tgactctgtt gacatcccga agtaatgctc agcgccagga aatctctgca
     6961 gcttttaaga ctctgtttgg cagggatctt ctggatgacc tgaaatcaga actaactgga
     7021 aaatttgaaa aattaattgt ggctctgatg aaaccctctc ggctttatga tgcttatgaa
     7081 ctgaaacatg ccttgaaggg agctggaaca aatgaaaaag tactgacaga aattattgct
     7141 tcaaggacac ctgaagaact gagagccatc aaacaagttt atgaagaaga atatggctca
     7201 agcctggaag atgacgtggt gggggacact tcagggtact accagcggat gttggtggtt
     7261 ctccttcagg ctaacagaga ccctgatgct ggaattgatg aagctcaagt tgaacaagat
     7321 gctcaggctt tatttcaggc tggagaactt aaatggggga cagatgaaga aaagtttatc
     7381 accatctttg gaacacgaag tgtgtctcat ttgagaaagg tgtttgacaa gtacatgact
     7441 atatcaggat ttcaaattga ggaaaccatt gaccgcgaga cttctggcaa tttagagcaa
     7501 ctactccttg ctgttgtgaa atctattcga agtatacctg cctaccttgc agagaccctc
     7561 tattatgcta tgaagggagc tgggacagat gatcataccc tcatcagagt catggtttcc
     7621 aggagtgaga ttgatctgtt taacatcagg aaggagttta ggaagaattt tgccacctct
     7681 ctttattcca tgattaaggg agatacatct ggggactata agaaagctct tctgctgctc
     7741 tgtggagaag atgacgaccc agctttcttg tacaaagtgg tgatatcacc ggtcgccacc
     7801 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     7861 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     7921 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     7981 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     8041 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     8101 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     8161 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     8221 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     8281 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     8341 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     8401 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     8461 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     8521 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg
     8581 cttggcgtaa tcatggtcat agcacccagc tttcttgtac aaagttgatg tagatccaga
     8641 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     8701 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     8761 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     8821 ggttttttaa ttcgcggccg cggcgccaat gcattgggcc ctcgagcata tgagatctgt
     8881 cccgatcttt ctcttctgtg ctgtcaattt agagtagttt ttgaggtaat ttgggtgcat
     8941 tctctgtaaa aacaacaaat aaatatttat attgtatgca ataaatgtaa caattacaca
     9001 tacactctct gattacagca ttaaagggtt agttcaccca aaaatgaaaa gatgtcatta
     9061 atgactcacc ctcatgtcgt tccaagcccg taacacctcc gttcatcttc ggaacacagt
     9121 ttaagatatt ttagatttag tccgagagct ttctgtccct ccactgaaaa tgtatgtacg
     9181 gtatactgtc catgtccaga aaggtaataa aaacatcaaa gtagtccatg tgacatcagt
     9241 gggttaggta gaattatttg aagcatcgaa aatacatttt ggtccaaaaa taacaaaacc
     9301 tacgacttta ttcagcattg tagcgtcact gcgaagtcgt gaacgcggat tgacaacaga
     9361 cccggaagag aatacaatgc cgaataaagt cgtaggtttt gttatttttg gaccaaaatg
     9421 tattcgatgc ttcaaaaaat tctaactaac ccactgatgt cacatggact actttgatgt
     9481 ttttattacc tttctggaca tggacagtat accgtacata cattctcaat ggaggcacag
     9541 aaagctctcg gactaaatct aaaatatctt aaactgtgtt ctgaagatga acggaggtct
     9601 tacgggcttg gaacgacatg agggcgagtc attaatgaca ttattttcat ttttgggtga
     9661 actaaccctt taataaaact gacatttgca gccaattgca cactttataa tgttaaatac
     9721 ttgttgattt tagaaataca gtagcattta gtgacaatca aacattaatt ttatttctga
     9781 ttatattgac aaaaacacag gactgtaagg ctgacatgac agggtgaatt gatttcatta
     9841 cattagtttt gatggtaaca cttttacaat aaggttcgtt agttaactac attagttaac
     9901 atgaactaat aatgaactgc acgtatacat gttaatttca acatttacta atactttata
     9961 tcttgttaac attagttaat gcactgtgaa ctaacatgaa caaacaatga acatctgtat
    10021 ttctattaac taacgttaac aaagattaac agatacagta acaaatgtat tgctcatggt
    10081 tatttaatgt tagttaatac atgaactaat caacctcatt gtaaagtgtt accgttttga
    10141 caatacaaca tgtttggaaa aaaatttctc gcttggtttt cttgtgtttt gcatgaattc
    10201 aaacacaatg agagcacgga atgataatga ttattcaaag ctgcaatagg cgccaaattt
    10261 cccgtggacc taacgttacc aattacaaat gcattttagc attttattac gtgagcctta
    10321 tattgtcaat cgcatgcatt agtgaaaata ggcctactac acatataaaa ggcgcgccat
    10381 caacgtcaaa acatgcacac ccaaaaaaaa aaaaaaaaaa aaaaaaaaaa actatcagta
    10441 aaacaaaata cttaatgtac ttacctcaat atgcttcctt aggtttgatg gcgaactttt
    10501 gaaggccgat atttctttat taagcgggag acagaggaca catttcatct tgaatgaatc
    10561 tttatttaca ccacttaaag aaaagaattc gcgaagatac ggccacgggt gctcttgatc
    10621 ctgtggctga ttttggactg tgctgctcgc agctgctgat gaatcacata cttcctccat
    10681 tttcttccac tgattgactg ttataatttc cctaatttcc aggtcaaggt gctgtgcatt
    10741 gtggtaatag atgtgacatg acgtcacttc caaaggacca atgaacatgt ctgaccaatt
    10801 tcatataatg tgaaaacgat tttcataggc agaataaata acatttaaat taaactgggc
    10861 atcagcgcaa ttcaattggt ttggtaatag caagggaaaa tagaatgaag tgatctccaa
    10921 aaaataagta ctttttgact gtaaataaaa ttgtaaggag taaaaagtac ttttttttct
    10981 aaaaaaatgt aattaagtaa aagtaaaagt attgattttt aattgtactc aagtaaagta
    11041 aaaatcccca aaaataatac ttaagtacag taatcaagta aaattactca agtactttac
    11101 acctctggtt cttgaccccc taccttcagc aagcccagca gatccactag ttctagagcg
    11161 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
    11221 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    11281 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    11341 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    11401 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    11461 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    11521 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    11581 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    11641 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    11701 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    11761 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    11821 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11881 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11941 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    12001 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    12061 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    12121 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    12181 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    12241 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    12301 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    12361 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    12421 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    12481 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    12541 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    12601 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    12661 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    12721 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    12781 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    12841 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    12901 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    12961 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    13021 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    13081 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    13141 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    13201 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    13261 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    13321 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtg
//
