LOCUS Exported 6089 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION AAV expression of EYFP from CaMKII(1.3) promoter. ACCESSION . VERSION . KEYWORDS pAAV.CamKII(1.3).eYFP.WPRE.hGH SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6089) TITLE Deisseroth AAV plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 6089) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6089 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 12..141 /label=AAV2 ITR promoter 160..1448 /label=CaMKII promoter /note="promoter for the mouse calcium/calmodulin-dependent kinase II alpha subunit" CDS 1472..2191 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1517..1538) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1778..1797) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2125..2146 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2216..2804 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2269..2289) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2687..2698) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 2836..3312 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3035..3054) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3352..3492 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3352..3481 /label=AAV2 ITR rep_origin 3567..4022 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3654..3673) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3864..3885 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4039..4058) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4158..4180 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4218..4236) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4304..4408 /gene="bla" /label=AmpR promoter CDS 4409..5269 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4627..4646) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5440..6028 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5929..5948 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt 181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt 241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt 301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact 361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca 421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc 481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat 541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc 601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat 661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct 721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc 781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga 841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac 901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc 961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa 1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat 1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc 1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa 1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt 1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga 1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca 1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg 1441 ggggcagctc tagagcggta ccggatccac catggtgagc aagggcgagg agctgttcac 1501 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 1561 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 1621 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca 1681 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 1741 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 1801 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 1861 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 1921 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 1981 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 2041 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa 2101 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 2161 cactctcggc atggacgagc tgtacaagta agaattcgat atcaagctta tcgataatca 2221 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 2281 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 2341 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 2401 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 2461 gggcattgcc accacctgtc agctcctttc cggaactttc gctttccccc tccctattgc 2521 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 2581 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgccta 2641 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 2701 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 2761 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgagcgct 2821 gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg 2881 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg 2941 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg 3001 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca 3061 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct 3121 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt 3181 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag 3241 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc 3301 ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc 3361 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac 3421 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca 3481 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac 3541 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg 3601 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 3661 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 3721 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 3781 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 3841 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 3901 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 3961 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat 4021 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca 4081 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 4141 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 4201 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 4261 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 4321 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 4381 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 4441 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 4501 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 4561 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 4621 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 4681 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 4741 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 4801 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 4861 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 4921 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 4981 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 5041 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 5101 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 5161 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 5221 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 5281 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 5341 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 5401 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 5461 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 5521 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 5581 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 5641 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 5701 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 5761 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 5821 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 5881 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 5941 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 6001 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 6061 ggccttttgc tggccttttg ctcacatgt //