LOCUS       Exported                1014 bp ds-DNA     linear   SYN 03-JUN-2021
DEFINITION  Mammalian expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA6 asyn A30P
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Mbefo MK, Fares MB, Paleologou K, Oueslati A, Yin G, Tenreiro S, 
            Pinto M, Outeiro T, Zweckstetter M, Masliah E, Lashuel HA
  TITLE     Parkinson disease mutant E46K enhances alpha-synuclein 
            phosphorylation in mammalian cell lines, in yeast, and in vivo.
  JOURNAL   J Biol Chem. 2015 Apr 10;290(15):9412-27. doi: 
            10.1074/jbc.M114.610774. Epub 2015 Feb 5.
  PUBMED    25657004
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(529..553)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(600..617)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    606..831
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tttgtttact ttagaaggan atatacatat ggatgtattc atgaaaggac tttcaaaggc
       61 caaggaggga gttgtggctg ctgctgagaa aaccaaacag ggtgtggcag aagcaccagg
      121 aaagacaaaa gagggtgttc tctatgtagg ctccaaaacc aaggagggag tggtgcatgg
      181 tgtggcaaca gtggctgaga agaccaaaga gcaagtgaca aatgttggag gagcagtggt
      241 gacgggtgtg acagcagtag cccagaagac agtggaggga gcagggagca ttgcagcagc
      301 cactggcttt gtcaaaaagg accagttggg caagaatgaa gaaggagccc cacaggaagg
      361 aattctggaa gatatgcctg tggatcctga caatgaggct tatgaaatgc cttctgagga
      421 agggtatcaa gactacgaac ctgaagccta agaaatatct ttgctcccag tttcttgaga
      481 tctgctgaca gatgttccat ccaagcttcg agaagggtgg gcgcgccgac ccagctttct
      541 tgtacaaagt ggtgataatt aattaagatc tagagggccc gtttaaaccc gctgatcagc
      601 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
      661 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
      721 ttgtctgagt aggtgtcatt ctattctggg gggtgggggt ggggcaggac agcaaggggg
      781 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
      841 cgganagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
      901 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      961 ccgctccttt cgctttcttc ncttcctttc tcgccacgtt cgccggcttt cccc
//
