LOCUS       Exported                4391 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Plasmid encoding moxVenus.
ACCESSION   .
VERSION     .
KEYWORDS    pEB1-moxVenus
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(200..228)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     terminator      924..1010
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1548..2498)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2546..2768
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      3260..3354
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3385..4179)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3496..3515)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4106..4125
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4358..4376
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 acagctaaca ccacgtcgtc cctatctgct gccctaggtc tatgagtggt tgctggataa
       61 ctttacgggc atgcataagg ctcgtatgat atattcaggg agaccacaac ggtttccctc
      121 tacaaataat tttgtttaac ttttctagat ttaagaagga gatatacata tgagtaaagg
      181 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      241 gcacaaattt tctgtccgtg gagagggtga aggtgatgca acaaacggaa aacttaccct
      301 taaattgatt tccactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      361 gggttatggt ctgcaatcgt ttgcgagata cccagatcat atgaaacagc atgacttttt
      421 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      481 gacctacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      541 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      601 ctttaactca cacaatgtat acatcacggc agacaaacaa aagaatggaa tcaaagcgaa
      661 cttcaaaatt agacacaacg ttgaagatgg aggtgttcaa ctagcagacc attatcaaca
      721 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtcctacca
      781 atctaagctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagcgagt
      841 aacagctgct gggattacac atggcatgga tgaactatac aaataaatgt ccagacctgc
      901 aggcatgcaa gctctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      961 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct
     1021 agacctaggg tacgggtttt gctgcccgca aacgggctgt tctggtgttg ctagtttgtt
     1081 atcagaatcg cagatccggc ttcagccggt ttgccggctg aaagcgctat ttcttccaga
     1141 attgccatga ttttttcccc acgggaggcg tcactggctc ccgtgttgtc ggcagctttg
     1201 attcgataag cagcatcgcc tgtttcaggc tgtctatgtg tgactgttga gctgtaacaa
     1261 gttgtctcag gtgttcaatt tcatgttcta gttgctttgt tttactggtt tcacctgttc
     1321 tattaggtgt tacatgctgt tcatctgtta cattgtcgat ctgttcatgg tgaacagctt
     1381 tgaatgcacc aaaaactcgt aaaagctctg atgtatctat cttttttaca ccgttttcat
     1441 ctgtgcatat ggacagtttt ccctttgata tgtaacggtg aacagttgtt ctacttttgt
     1501 ttgttagtct tgatgcttca ctgatagata caagagccat aagaacctca gatccttccg
     1561 tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa
     1621 ccattgagat catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc
     1681 tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttatg taggtaggaa
     1741 tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt
     1801 tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg
     1861 cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt
     1921 ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc aagcattaac
     1981 atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt
     2041 agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca
     2101 tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca
     2161 ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc
     2221 tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt
     2281 gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg agcgtattgg
     2341 ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt
     2401 agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc
     2461 gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga
     2521 ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt
     2581 tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct
     2641 agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt
     2701 ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg
     2761 tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt
     2821 gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg
     2881 ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt
     2941 tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta
     3001 caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa agcccgtcac
     3061 gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt
     3121 tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt
     3181 cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct
     3241 tactgtccct agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     3301 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     3361 agctctcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
     3421 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     3481 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     3541 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     3601 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag
     3661 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     3721 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     3781 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     3841 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     3901 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     3961 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga
     4021 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     4081 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     4141 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     4201 ctcttgatca gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca
     4261 gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca attccgacgt
     4321 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     4381 tcgtcttcac c
//
