LOCUS       Exported                9706 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  dCas9 plasmid expressing 2A sRNA trigger.
ACCESSION   .
VERSION     .
KEYWORDS    PAJ389
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Galizi R, Duncan JN, Rostain W, Quinn CM, Storch M, Kushwaha M, 
            Jaramillo A
  TITLE     Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and
            Diagnostics.
  JOURNAL   CRISPR J. 2020 Oct;3(5):398-408. doi: 10.1089/crispr.2020.0029.
  PUBMED    33095053
REFERENCE   2  (bases 1 to 9706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   40..75
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     primer_bind     complement(504..523)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(2376..3035)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2950..2969
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3036..3138)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    3523..3541
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3548..3566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(3910..3927)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4044..4589)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4124..4143)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        4701..4729
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_RNA        complement(5047..5125)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             5421..9527
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    9548..9679
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   9680..9
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
ORIGIN
        1 tcccaaaact gagaccagtc tcggaagctc aaaggtctcg ttttagagct atgctgtttt
       61 gaatggtccc aaaacttcag cacactgaga cttgttgagt tccatgtttt agagctatgc
      121 tgttttgaat ggactccatt caacattgcc gatgataact tgagaaagag ggttaatacc
      181 agcagtcgga taccttccta ttctttctgt taaagcgttt tcatgttata ataggcaaaa
      241 gaagagtagt gtgatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
      301 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
      361 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
      421 ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg
      481 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      541 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      601 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
      661 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
      721 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
      781 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
      841 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
      901 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
      961 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     1021 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     1081 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     1141 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     1201 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     1261 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     1321 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     1381 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg
     1441 cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc
     1501 gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc
     1561 tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc
     1621 gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt
     1681 gtttcgtaaa gtctggaaac gcggaagtcc cctacgtgct gctgaagttg cccgcaacag
     1741 agagtggaac caaccggtga taccacgata ctatgactga gagtcaacgc catgagcggc
     1801 ctcatttctt attctgagtt acaacagtcc gcaccgctgc cggtagctcc ttccggtggg
     1861 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     1921 gacaggtgcc ggcagcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
     1981 cgaaacaagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac
     2041 cctgtggaac acctacatct gtattaacga agcgctaacc gtttttatca ggctctggga
     2101 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2161 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2221 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2281 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca
     2341 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg
     2401 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     2461 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     2521 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     2581 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
     2641 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     2701 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     2761 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat
     2821 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     2881 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     2941 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     3001 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     3061 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     3121 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccgggg cttcccggta
     3181 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat
     3241 tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt
     3301 ttgaggtgct ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacag
     3361 cttgattcta gctgatcgtg gaccggaagg tgagccagtg agttgattgc agtccagtta
     3421 cgctggagtc tgaggctcgt cctgaatgat atgcgaccgc cggagggttg cgtttgagac
     3481 gggcgacaga tcgacactgc tcgatccgct cgcaccgaat tctccctatc agtgatagag
     3541 attgacatcc ctatcagtga tagagatact gagcactcat taaccatacc cagggtattc
     3601 cggacttctt caaacatcct tagcgaaagc taaggatttt ttttctgcag ggatcgacga
     3661 gagcagcgcg actggatcag ttctggacga gcgagctgtc gtccgacccg tgatcttacg
     3721 gcattatacg tatgatcggg ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc
     3781 tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc
     3841 atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata
     3901 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat
     3961 ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta acagggaagt
     4021 gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
     4081 atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4141 ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     4201 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
     4261 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc
     4321 cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc
     4381 cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg
     4441 aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg
     4501 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag
     4561 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt
     4621 tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc cccatacgat
     4681 ataagttgta attctaatgt ttgacagctt atcatcgata agctttaatg cggtagttta
     4741 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
     4801 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
     4861 ccgggcctct tgcgggatta cgaaatcatc ctgtggagct tagtaggttt agcaagatgg
     4921 cagcgcctaa atgtagaatg ataaaaggat taagagatta atttccctaa aaatgataaa
     4981 acaagcgttt tgaaagcgct tgtttttttg gtttgcagtc agagtagaat agaagtatca
     5041 aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac
     5101 ttgctatgct gttttgaatg gttccaacaa gattatttta taacttttat aacaaataat
     5161 caaggagaaa ttcaaagaaa tttatcagcc ataaaacaat acttaatact atagaatgat
     5221 aacaaaataa actacttttt aaaagaattt tgtgttataa tctatttatt attaagtatt
     5281 gggtaatatt ttttgaagag atattttgaa aaagaaaaat taaagcatat taaactaatt
     5341 tcggaggtca ttaaaactat tattgaaatc atcaaactca ttatggattt aatttaaact
     5401 ttttatttta ggaggcaaaa atggataaga aatactcaat aggcttagct atcggcacaa
     5461 atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg
     5521 ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg
     5581 acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac
     5641 gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag
     5701 atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg
     5761 aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa
     5821 ctatctatca tctgcgaaaa aaattggtag attctactga taaagcggat ttgcgcttaa
     5881 tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt gagggagatt
     5941 taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa acctacaatc
     6001 aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg attctttctg
     6061 cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc ggtgagaaga
     6121 aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct aattttaaat
     6181 caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact tacgatgatg
     6241 atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt ttggcagcta
     6301 agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact gaaataacta
     6361 aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa gacttgactc
     6421 ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc ttttttgatc
     6481 aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa gaattttata
     6541 aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg gtgaaactaa
     6601 atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt ccccatcaaa
     6661 ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat ccatttttaa
     6721 aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat tatgttggtc
     6781 cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa gaaacaatta
     6841 ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca tttattgaac
     6901 gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa catagtttgc
     6961 tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt actgaaggaa
     7021 tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat ttactcttca
     7081 aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa aaaatagaat
     7141 gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca ttaggtacct
     7201 accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa gaaaatgaag
     7261 atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag atgattgagg
     7321 aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag cttaaacgtc
     7381 gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt agggataagc
     7441 aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat cgcaatttta
     7501 tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt
     7561 ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc cctgctatta
     7621 aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc
     7681 ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact caaaagggcc
     7741 agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc
     7801 agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag ctctatctct
     7861 attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt aatcgtttaa
     7921 gtgattatga tgtcgatgcc attgttccac aaagtttcct taaagacgat tcaatagaca
     7981 ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag
     8041 aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag ttaatcactc
     8101 aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa cttgataaag
     8161 ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa
     8221 ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt cgagaggtta
     8281 aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc caattctata
     8341 aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat gccgtcgttg
     8401 gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat ggtgattata
     8461 aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc aaagcaaccg
     8521 caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt acacttgcaa
     8581 atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga gaaattgtct
     8641 gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc caagtcaata
     8701 ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa
     8761 gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt
     8821 ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa aaagggaaat
     8881 cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa agaagttcct
     8941 ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt aaaaaagact
     9001 taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt aaacggatgc
     9061 tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc aaatatgtga
     9121 attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa gataacgaac
     9181 aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt gagcaaatca
     9241 gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt cttagtgcat
     9301 ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt catttattta
     9361 cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca attgatcgta
     9421 aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa tccatcactg
     9481 gtctttatga aacacgcatt gatttgagtc agctaggagg tgactgaagt atattttaga
     9541 tgaagattat ttcttaataa ctaaaaatat ggtataatac tcttaataaa tgcagtaata
     9601 caggggcttt tcaagactga agtctagctg agacaaatag tgcgattacg aaatttttta
     9661 gacaaaaata gtctacgagg ttttagagct atgctgtttt gaatgg
//
