LOCUS       Exported                6258 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLTR-RD114A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Zhang XY, La Russa VF, Reiser J
  TITLE     Transduction of bone-marrow-derived mesenchymal stem cells by using 
            lentivirus vectors pseudotyped with modified RD114 envelope 
            glycoproteins.
  JOURNAL   J Virol. 2004 Feb . 78(3):1219-29.
  PUBMED    14722277
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    3017..3138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3054..3073)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3108..3127
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3145..3600)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3282..3303)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3494..3513
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3627..3731
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3733..4090
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(3756..3776)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3941..4076
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4003..4022
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4125..4919
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4179..4198)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4789..4808
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5107..5126)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5151..5198
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5527..6115
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6016..6035
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaacaatgc tgcttgtgcc
       61 tggctagaag cacaagagga ggaagaggtg ggttttccag tcacacctca ggtaccttta
      121 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
      181 ctggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
      241 acacaaggct acttccctga ttggcagaac tacacaccag ggccaggggt cagatatcca
      301 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      361 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatggaat ggatgaccct
      421 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      481 gagctgcatc cggagtactt caagaactgc tgacatcgag cttgctacaa gggactttcc
      541 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      601 tgctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      661 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagctgatc
      721 ccccgggctg caggaattta tgaaatcctt tatgggggac cccccccttt gtcaaccttg
      781 ctcaattcct tctccccctc cgatcctaag actgatttac aagcccgact aaaagggctg
      841 caagcggtgc aggcccaaat ctggacaccc ctggccgaat tgtaccggcc aggacatcca
      901 caaactagcc acccatttca ggtgggagac tccgtgtacg tccggcggca ccgctctcaa
      961 ggattggagc ctcgttggaa gggaccttac atcgtcctgc tgaccacgcc caccgccata
     1021 aaggttgacg ggatcgccgc ctggattcac gcatcgcacg ccaaggcagc cccaaaaacc
     1081 cctggaccag aaactcccaa aacctggaag ctccgccgtt cggagaaccc tcttaagata
     1141 agactctccc gtgtctgact gctaatccac cttgtccctg tactaaccca aaatgaaact
     1201 cccaacagga atggtcattt tatgtagcct aataatagtt cgggcagggt ttgacgaccc
     1261 ccgcaaggct atcgcattag tacaaaaaca acatggtaaa ccatgcgaat gcagcggagg
     1321 gcaggtatcc gaggccccac cgaactccat ccaacaggta acttgcccag gcaagacggc
     1381 ctacttaatg accaaccaaa aatggaaatg cagagtcact ccaaaaaatc tcacccctag
     1441 cgggggagaa ctccagaact gcccctgtaa cactttccag gactcgatgc acagttcttg
     1501 ttatactgaa taccggcaat gcagggcgaa taataagaca tactacacgg ccaccttgct
     1561 taaaatacgg tctgggagcc tcaacgaggt acagatatta caaaacccca atcagctcct
     1621 acagtcccct tgtaggggct ctataaatca gcccgtttgc tggagtgcca cagcccccat
     1681 ccatatctcc gatggtggag gacccctcga tactaagaga gtgtggacag tccaaaaaag
     1741 gctagaacaa attcataagg ctatgcatcc tgaacttcaa taccacccct tagccctgcc
     1801 caaagtcaga gatgacctta gccttgatgc acggactttt gatatcctga ataccacttt
     1861 taggttactc cagatgtcca attttagcct tgcccaagat tgttggctct gtttaaaact
     1921 aggtacccct acccctcttg cgatacccac tccctcttta acctactccc tagcagactc
     1981 cctagcgaat gcctcctgtc agattatacc tcccctcttg gttcaaccga tgcagttctc
     2041 caactcgtcc tgtttatctt cccctttcat taacgatacg gaacaaatag acttaggtgc
     2101 agtcaccttt actaactgca cctctgtagc caatgtcagt agtcctttat gtgccctaaa
     2161 cgggtcagtc ttcctctgtg gaaataacat ggcatacacc tatttacccc aaaactggac
     2221 aggactttgc gtccaagcct ccctcctccc cgacattgac atcatcccgg gggatgagcc
     2281 agtccccatt cctgccattg atcattatat acatagacct aaacgagctg tacagttcat
     2341 ccctttacta gctggactgg gaatcaccgc agcattcacc accggagcta caggcctagg
     2401 tgtctccgtc acccagtata caaaattatc ccatcagtta atatctgatg tccaagtctt
     2461 atccggtacc atacaagatt tacaagacca ggtagactcg ttagctgaag tagttctcca
     2521 aaataggagg ggactggacc tactaacggc agaacaagga ggaatttgtt tagccttaca
     2581 agaaaaatgc tgtttttatg ctaacaagtc aggaattgtg agaaacaaaa taagaaccct
     2641 acaagaagaa ttacaaaaac gcagggaaag cctggcatcc aaccctctct ggaccgggct
     2701 gcagggcttt cttccgtacc tcctacctct cctgggaccc ctactcaccc tcctactcat
     2761 actaaccatt gggccatgcg ttttcaatcg attggtccaa tttgttaaag acaggatctc
     2821 agtggtccag gctctggttt tgactcagca atatcaccag ctaaaaccca tagagtacga
     2881 gccatgaaga tccaccggat ctagataact gatcataatc agccatacca catttgtaga
     2941 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     3001 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3061 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3121 actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     3181 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     3241 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     3301 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3361 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     3421 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     3481 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     3541 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     3601 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3661 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3721 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     3781 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3841 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3901 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3961 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4021 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4081 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     4141 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     4201 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     4261 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     4321 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     4381 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     4441 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     4501 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     4561 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     4621 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     4681 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     4741 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     4801 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     4861 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     4921 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     4981 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     5041 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     5101 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     5161 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     5221 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     5281 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     5341 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     5401 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5461 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5521 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5581 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5641 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     5701 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5761 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5821 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5881 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5941 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6001 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6061 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6121 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6181 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattacc
     6241 ggactcagat ctcgagac
//
