LOCUS Exported 10728 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses mouse Fzd3 in mammalian cells. ACCESSION . VERSION . KEYWORDS pLEX307-mFzd3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10728) AUTHORS Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R, Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH TITLE Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates. JOURNAL Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509. PUBMED 28885975 REFERENCE 2 (bases 1 to 10728) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10728 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 93..1271 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 324..1262 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1219..1239 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1291..1315 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter complement(3388..3405) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 3410..3544 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind complement(3634..3658) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3666..3707 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 3758..4346 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3811..3831) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4229..4240) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 4418..4651 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 4729..4850 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 4890..5025 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(5045..5064) /label=T7 /note="T7 promoter, forward primer" promoter complement(5046..5064) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5074..5091) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(5074..5090) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5083..5105) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 5232..5687 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5319..5338) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5529..5550 /label=F1ori-F /note="F1 origin, forward primer" promoter 5713..5817 /gene="bla" /label=AmpR promoter CDS 5818..6678 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6036..6055) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6849..7437 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7338..7357 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7591..7608 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7725..7746 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7761..7791 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7799..7815 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7804..7826 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7823..7839 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7823..7839 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7858..7878 /label=T3 /note="T3 promoter, forward primer" promoter 7860..7878 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 7906..8132 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 8133..8313 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 8360..8485 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8978..9211 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9396..9440 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 9671..9881 /label=SV40 promoter /note="SV40 early promoter" promoter 9693..9889 /label=SV40 promoter /note="SV40 early promoter" rep_origin 9740..9875 /label=SV40 ori /note="SV40 origin of replication" CDS 9921..10523 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS" primer_bind complement(9921..9940) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 10417..10437 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 10566..10683 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 gaggcctgca ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat 61 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc 121 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct 181 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 241 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 301 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct 361 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc 421 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag 481 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa 541 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt 601 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag 661 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc 721 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt 781 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc 841 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc 901 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg 961 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca 1021 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt 1081 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga 1141 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt 1201 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc 1261 aggtgtcgtg aggctagcat cgattgatca acaagtttgt acaaaaaagc aggctggcgc 1321 cggaaccaat tcagtcgact ggatccggta ccgaattcag gccggcctac catggctgtg 1381 agctggattg tctttgatct ttggctcttg actgtgtttc tggggcagat aggtgggcac 1441 agtttgtttt cttgtgaacc tataaccttg aggatgtgcc aagatttgcc ttacaatact 1501 accttcatgc ctaatcttct gaaccattat gaccaacaga ctgcagcttt agcaatggag 1561 cccttccacc ctatggtgaa cctggattgt tctcgggatt ttcggccatt tctttgtgca 1621 ctctatgccc ctatttgtat ggaatatgga cgtgtcacac ttccctgccg taggctgtgt 1681 cagcgtgcct atagcgagtg ttcaaaactc atggagatgt ttggtgtccc gtggcctgaa 1741 gatatggagt gcagtaggtt tccagattgt gatgagccat atccccgact tgtggatttg 1801 aatttagttg gagatccaac tgaaggagcc ccagttgcag tgcagaggga ctatggtttt 1861 tggtgtccca gagagttaaa aattgatcct gatcttggct attcctttct gcacgtgcga 1921 gattgttcgc caccatgtcc caatatgtac ttcaggagag aagaactgtc atttgctcgc 1981 tatttcatag gcctgatttc aatcatttgc ctctctgcca cattgtttac ttttttaacc 2041 tttctaattg acgtcacaag attccgttac cctgaaagac ctatcatatt ttatgcagtc 2101 tgctacatga tggtgtcatt aattttcttc attgggtttt tgctggagga ccgagtagcc 2161 tgcaatgcat ctagccctgc acagtataag gcttctacag tgacacaagg atctcacaat 2221 aaggcctgta ccatgctctt tatggtacta tattttttca ctatggctgg cagtgtatgg 2281 tgggtaattc ttaccatcac atggttttta gcagctgtgc caaagtgggg cagtgaagct 2341 attgagaaga aagcattgct gtttcatgcc agtgcctggg gcatccccgg aactctaact 2401 atcatccttt tagcgatgaa taaaattgaa ggtgacaata ttagtggcgt gtgttttgtc 2461 ggcctctacg acgttgatgc attaagatat ttcgttctcg ctcccctctg cctgtatgtg 2521 gtagttgggg tttctctcct tttagccggc attatatccc taaacagagt tcggattgag 2581 atcccattag aaaaggaaaa ccaagataag ttagtgaagt tcatgatccg gattggtgtt 2641 ttcagcattc tctaccttgt gccactcttg gttgtaattg gatgttactt ttatgagcaa 2701 gcttaccgcg gcatctggga gacaacatgg atccaggaac gctgcagaga gtatcacatt 2761 ccatgtccgt accaggttac tcagatgagt cgtccagacc tgattctctt tctgatgaag 2821 tatctcatgg ctctcatagt tgggattccc tctatatttt gggttggaag caaaaagaca 2881 tgctttgaat gggccagttt tttccatggg cgtaggaaaa aagagatagt gaatgagagc 2941 cggcaggtgc tccaggaacc tgactttgct cagtcactcc tgagggaccc aaatactcca 3001 attataagaa aatcaagagg aacttccact caagggacat ccacacatgc ttcttcaact 3061 cagctggcca tggtggatga ccaaagaagc aaagcaggga gtgtccacag caaagtgagc 3121 agctaccatg gcagcctcca caggtcacgg gatggcaggt acactccctg cagttaccga 3181 ggaatggagg agagactacc tcacggcagc atgtcacggc tgacggatca ttccaggcac 3241 agtagttctc atcggctcaa cgagcagtcc cgacacagca gcatccgaga cctcagtaac 3301 aaccccatga ctcacattac acatggcacc agcatgaacc gtgttattga ggaggatgga 3361 accagtgctt agggcgcgcc tctagaacta tagtgagtcg tattacgtag atccagacat 3421 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt 3481 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca 3541 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt 3601 tttttaattc gcggccgcac tcgagatatc tagacccagc tttcttgtac aaagtggttg 3661 atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgaacta 3721 gtaccggtta agtcgacaat caacgcgtta agtcgacaat caacctctgg attacaaaat 3781 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 3841 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 3901 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 3961 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 4021 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 4081 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 4141 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct 4201 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 4261 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 4321 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct 4381 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 4441 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 4501 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 4561 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 4621 cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt 4681 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 4741 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 4801 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 4861 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 4921 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 4981 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa 5041 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga 5101 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 5161 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 5221 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 5281 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 5341 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 5401 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 5461 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 5521 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 5581 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 5641 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 5701 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 5761 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 5821 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 5881 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 5941 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 6001 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 6061 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 6121 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 6181 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 6241 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 6301 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 6361 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 6421 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 6481 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 6541 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 6601 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 6661 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 6721 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 6781 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 6841 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 6901 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 6961 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 7021 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 7081 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 7141 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 7201 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 7261 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 7321 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 7381 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 7441 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 7501 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 7561 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 7621 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 7681 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 7741 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 7801 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca 7861 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa 7921 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga 7981 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa 8041 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata 8101 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag 8161 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 8221 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 8281 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc 8341 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc 8401 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa 8461 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga 8521 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc 8581 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg 8641 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc 8701 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac 8761 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca 8821 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat 8881 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga 8941 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct 9001 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac 9061 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac 9121 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg 9181 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca 9241 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt 9301 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac 9361 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat 9421 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa 9481 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt 9541 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa 9601 ccccgagggg acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 9661 aggctcccca gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt 9721 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 9781 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 9841 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag 9901 aggtaccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg 9961 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc 10021 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc 10081 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg 10141 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc 10201 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg 10261 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc 10321 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg 10381 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc 10441 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga 10501 cccgcaagcc cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc 10561 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac 10621 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat 10681 tttcgggttt attacaggga cagcagagat ccactttggc gccggctc //