LOCUS       Exported                8334 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses Venus-PSD-95 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-5U-Venus-PSD-95-3U
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Ifrim MF, Williams KR, Bassell GJ
  TITLE     Single-Molecule Imaging of PSD-95 mRNA Translation in Dendrites and 
            Its Dysregulation in a Mouse Model of Fragile X Syndrome.
  JOURNAL   J Neurosci. 2015 May 6;35(18):7116-30. doi: 
            10.1523/JNEUROSCI.2802-14.2015.
  PUBMED    25948262
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      918..927
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             954..1670
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(999..1020)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1260..1279)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1607..1628
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1677..1686
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    5122..5243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5159..5178)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5213..5232
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5250..5705)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5387..5408)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5599..5618
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5732..5836
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5838..6195
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(5861..5881)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6046..6181
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6108..6127
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6230..7024
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6284..6303)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6894..6913
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7212..7231)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7256..7303
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7632..8220
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8121..8140
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccagc
      601 tcatgcccca gccccagaac tccggggctg ccagccccct gtgcccctac ccctcctgag
      661 aatcggggtg tgctcctcaa gccccctccc ctccactggg gagccccttt tagggtcccc
      721 cctcccttcc acgctcccct acccttctct tctccaacct tctccttccc ctctcacccc
      781 tccccccatc ctggcccccc ctgcaaaagt ggggtgcgga ggggggaggg gtgagaaccc
      841 accgagggga ggaacaaaac tccaatgaag tcagagcccc ctactcgccg ccgcggccag
      901 gccccccaac accggtcgcc accatggact gtctctgtat agtgacaacc aagatggtga
      961 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1021 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1081 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1141 ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     1201 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1261 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1321 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1381 agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     1441 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     1501 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1561 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1621 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggcgcggccg
     1681 ccatggactg tctctgtata gtgacaacca agaaataccg ctaccaagat gaagacacgc
     1741 cccctctgga acacagcccg gcccacctcc ccaaccaggc caattctccc cctgtgattg
     1801 tcaacacgga caccctagaa gccccaggat atgtgaacgg aacagagggg gagatggagt
     1861 atgaggagat cacattggaa aggggtaact caggtctggg cttcagcatc gcaggtggca
     1921 ccgacaaccc acacatcggt gacgacccat ccatctttat caccaagatc attcctggtg
     1981 gggctgcagc ccaggatggc cgcctcaggg tcaacgacag catcctgttt gtcaatgaag
     2041 tggatgtccg ggaggtgacc cattcagctg cagtggaggc cctcaaagag gcgggttcca
     2101 tcgttcgcct ctacgtcatg cgccggaaac ccccagctga gaagatcata gagatcaagc
     2161 ttatcaaagg gcctaaagga cttggcttca gcatcgcagg gggcgttggg aaccagcaca
     2221 tccctggaga taatagcatc tacgtaacca agatcatcga aggaggcgct gcccacaagg
     2281 atggcaggtt gcagatcgga gacaagatcc tggcggtcaa cagtgtgggg ctagaggatg
     2341 tcatgcatga ggacgccgtg gcagccctga agaacacata tgacgttgtg tacctaaagg
     2401 tggccaagcc cagcaatgcc tacctgagtg acagctatgc tcccccagac atcacaacct
     2461 catattctca gcacctggac aatgagatca gtcatagcag ctacttgggc accgactacc
     2521 ccacagccat gacccccact tcccctcggc gctactcccc cgtggccaag gacctgctag
     2581 gggaggaaga tattccccgg gaaccaaggc ggatcgtgat ccatcggggc tccaccggcc
     2641 tgggcttcaa cattgtgggc ggcgaggacg gtgaaggcat cttcatctcc ttcatccttg
     2701 ctgggggtcc agctgacctc agtggggagc tacggaaggg ggaccagatc ctgtcggtca
     2761 atggtgttga cctccgcaat gccagtcatg aacaggctgc cattgccctg aagaacgcgg
     2821 gtcagacggt cacgatcatc gctcagtata aaccagaaga gtatagccga ttcgaggcca
     2881 agatccatga tcttcgggaa cagcttatga atagtagcct gggctcaggg actgcatctc
     2941 tgcgaagcaa ccccaagcgg ggcttctata tcagggccct gtttgactac gacaagacca
     3001 aggactgcgg tttcttgagc caggccctga gcttccactt tggggatgtg cttcatgtaa
     3061 ttgacgccag cgacgaagag tggtggcaag cgcggcgggt ccactctgac agtgagaccg
     3121 atgacattgg cttcattccc agcaaacggc gggtcgagcg acgagagtgg tcaaggttaa
     3181 aggccaagga ctggggctcc agctctggat cacagggtcg agaagactcg gttctgagct
     3241 atgagacggt gacgcagatg gaagtgcact acgctcgccc catcatcatc cttgggccta
     3301 ccaaagaccg tgccaacgat gatcttctct ccgagttccc cgacaagttt ggatcctgtg
     3361 tccctcatac gacacgtcct aagcgggaat atgagataga cggccgcgat taccactttg
     3421 tctcctcccg ggagaaaatg gagaaggaca ttcaggcgca caagttcatt gaggctggcc
     3481 agtacaacag ccacctctac gggaccagcg tccagtctgt gcgagaggta gcagagcagg
     3541 ggaagcactg catccttgat gtctcagcca atgccgtgcg gcggctgcag gcggcccacc
     3601 tgcaccctat cgccatcttc atccgtcccc gctccctgga gaatgtgcta gagatcaata
     3661 agcggatcac agaggagcaa gcccggaaag ccttcgacag ggccacgaag ctggagcagg
     3721 agttcacgga gtgcttctca gccatcgtag agggcgacag ctttgaagag atctatcaca
     3781 aagtgaaacg tgtcatcgaa gacctctcag gcccctacat ctgggtccca gcccgagaga
     3841 gactctgaac tagtgggatc cgccctgaaa aagggctcga gccctgaaaa agggcaattg
     3901 ccctgaaaaa gggcgtcgac gccctgaaaa agggcagatc taacgttttc ctgccctggc
     3961 ttggcctgga ctcaccctgc ctccaccacc tgggcccttg gtctggactg aatagcccaa
     4021 gcccttcgta ccctagcctt tcttcccacc cttcttattt atttcctttc tgactggatc
     4081 gagcccattg gagggaggac actcctctct gcatgtatcc ctgcacccca gatcagggcc
     4141 cctagacccc aggaacctgg ggtctggggg ggagctgggc tcctggttcc gagcccttgc
     4201 tccttaagac tcctacccct gcccgccccc atgcacacac agacccactg ggggcctctg
     4261 ccctccccat tttctcccac acacattcca gaagtcaggg cccctctcaa ggagtacccg
     4321 ctgtagggat gcagggccac aggcttccgc gctctcccga ggcaggggct ggggtcaccc
     4381 ctgccccatc ataactcccc acgccgtttg agttctcctt tattttctcc attcttttct
     4441 tctcaaaggt ggtttttggg gggagaagca ggggggctct cctgagggtc cccccgtctt
     4501 tcacacacct cccacctttt ttctttgccg gtttgcatga gtggaaggtc taactgtggc
     4561 tttttttttt tctgggattt tatttcattt tatttgattt tatttttttg ggaaaagggg
     4621 agggatgggt ctggggagtg gggaatgtgg gaggggggtg ggggggcaaa gggtcagggg
     4681 ttgggtgtcc gggagccagg ggaggacagg aaatgctgcc gccttctgca atttatttat
     4741 ttttttcttt tgagagagtg aaaggaagag acagacactt gaataaaggt accagcttgt
     4801 ggctctgtgg ctttgctggg tgtggggagc aggttgggtg ggtgtggagc tctattgggg
     4861 ggggcatcta gggtgggcta ggccttgctg atggtatcta gtgggagggc tacaggccct
     4921 ttaatgaagt ctcacaaata accacacctt cagactgtct tgaagagttg aaaaggtact
     4981 cctccatccg gaagatccac cggatctaga taactgatca taatcagcca taccacattt
     5041 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     5101 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     5161 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5221 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt
     5281 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     5341 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     5401 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     5461 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     5521 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     5581 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     5641 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     5701 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5761 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5821 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     5881 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5941 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6001 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     6061 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6121 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6181 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     6241 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     6301 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     6361 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     6421 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     6481 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     6541 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     6601 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     6661 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     6721 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     6781 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     6841 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     6901 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     6961 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     7021 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     7081 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     7141 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     7201 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     7261 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     7321 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     7381 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     7441 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     7501 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7561 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7621 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7681 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7741 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7801 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7861 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7921 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7981 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8041 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8101 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8161 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8221 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8281 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
