LOCUS       Exported                1749 bp ds-DNA     linear   SYN 02-AUG-2021
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR75; Opto-GPR75; C10).
ACCESSION   .
VERSION     .
KEYWORDS    Opto-GPR75
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 2, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtctttt tcgatccagc cttcaggaaa
      301 ttcagaacca acctcaacta catcctgctc aacctggccg tggccgacct cttcatggtg
      361 ttcgggggct tcaccaccac cctctacacc tctctgcacg ggtacttcgt ctttgggccc
      421 acgggctgca acctggaggg cttctttgcc accttgggcg gtgaaattgc actgtggtcc
      481 ttggtggtcc tggccatcga gcggtacgtg gtggtgttgg ggaaacagcc taatcgcacg
      541 gcctcctttc cctgcgccat catgggcgtc gccttcacct gggtcatggc tctggcctgt
      601 gccgcgcccc ccctcgtcgg ctggtccagg tacatcccgg agggcatgca gtgctcgtgc
      661 gggattgact actacacgcc ccacgaggaa accaacaatg agtcgttcgt catctacatg
      721 ttcgtggtcc acttcatcat ccccctgatt gtcatattct tctgctacgg aatgattgct
      781 cagaccctgc ggaagaacgc tcaagtcaga aagtgccccc ctgtaatcac agtcgatgct
      841 tccagaccac agcctttcat gggggtccct gtgcagggag gtggagatcc catccagtgt
      901 gccatgccgg ctctgtatag gaaccagaat tacaacaaac tgcagcacgt tcagacccgt
      961 ggatatacca agagtcccaa ccaactggtc acccctgcag caagccgact ccagctcgta
     1021 tcagccatca acctctccac tgccaaggat tccaaagccg tgcgcatggt gatcatcatg
     1081 gtcatcgctt tcctaatctg ctggctgccc tacgctgggg tggcgttcta catcttcacc
     1141 catcagggct ctgactttgg ccccatcttc atgaccatcc cggctttctt tgccaagact
     1201 tctgccgtct acaaccccgt catctacatc atgatgaaca agcagttccg caggaaagtg
     1261 ctctggtgcc tccaatacat aggcctgggt tttttctgct gcaaacaaaa gactcgactt
     1321 cgagccatgg gaaaagggaa cctcgaagtc aacagaaaca aatcctccca tcatgaaaca
     1381 aactctgcct acatgttatc tccaaagcca cagaagaaat ttgtggacca ggcttgtggc
     1441 ccaagtcatt caaaagaaag tatggtgagt cccaagatct ctgctggaca tcaacactgt
     1501 ggtcagagca gctcgacccc catcaacact cggattgaac cttactacag catctataac
     1561 agcagccctt cccaggagga gagcagccca tgtaacttac agccagtaaa ctcttttgga
     1621 tttgccaatt catatattgc catgcattat cacaccacta atgacttagt gcaggaatat
     1681 gacagcactt cagccaagca gattccagtc ccctccgtta cagaaaccag ccaagtggcg
     1741 cctgcctaa
//
