LOCUS       Exported                1143 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR146; Opto-GPR146; E1).
ACCESSION   .
VERSION     .
KEYWORDS    Opto-GPR146
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcaacc tacacagcaa ggccagcatg
      301 accatgctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg gcactgccgc ggacctacat ggccagcgtg
      541 tacaacgcca tcatgggcgt cgccttcacc tgggtcatgg ctctggcctg tgccgcgccc
      601 cccctcgtcg gctggtccag gtacatcccg gagggcatgc agtgctcgtg cgggattgac
      661 tactacacgc cccacgagga aaccaacaat gagtcgttcg tcatctacat gttcgtggtc
      721 cacttcatca tccccctgat tgtcatattc ttctgctacg gactactctc ccgcgtccgc
      781 agggaggaca cgcccctgga ccgggacacg ggccggctgg agccctcggc acacaggcgc
      841 atggtgatca tcatggtcat cgctttccta atctgctggc tgccctacgc tggggtggcg
      901 ttctacatct tcacccatca gggctctgac tttggcccca tcttcatgac catcccggct
      961 ttctttgcca agacttctgc cgtctacaac cccgtcatct acatcatgat gaacaagcag
     1021 ttccgcagca agctccaacg gctgatgaaa aagctgccct gcggggaccg gcactgctcc
     1081 ccggaccaca tgggggtgca gcaggtgctg gcgacagaaa ccagccaagt ggcgcctgcc
     1141 taa
//
