LOCUS Exported 2980 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Mobius Assembly Level 2 Acceptor Vector Gamma. ACCESSION . VERSION . KEYWORDS pMA_Level 2-Gamma SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2980) AUTHORS Andreou AI, Nakayama N TITLE Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly. JOURNAL PLoS One. 2018 Jan 2;13(1):e0189892. doi: 10.1371/journal.pone.0189892. eCollection 2018. PUBMED 29293531 REFERENCE 2 (bases 1 to 2980) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2980 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 8..65 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." terminator 87..114 /label=T7Te terminator /note="phage T7 early transcription terminator" terminator 130..201 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" RBS 932..943 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" terminator 1021..1064 /label=bacterial terminator /note="putative bacterial transcription terminator" primer_bind complement(1068..1086) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" CDS 1248..1907 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1314..1333) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" terminator 1928..2022 /label=lambda t0 terminator /note="transcription terminator from phage lambda" rep_origin 2205..2793 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2694..2713 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2947..2964 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 gaagtaatct tttcggtttt aaagaaaaag ggcagggtgg tgacaccttg cccttttttg 61 ccggactgca ggggtatatg caggtgcgca gaaaggccca cccgaaggtg agccagtgtg 121 actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga 181 ctgagccttt cgttttattt gaagctcatt acttatacag ctcgtccata ccgtgggtga 241 tgcccgcagc ggtcacaaat tccagcagga ccatgtgatc acgtttttcg ttcggatctt 301 tgctcagcac gctttgggtg ctcagatagt gattatccgg cagcaaaacc ggaccgtcac 361 caattggggt gttctgctgg taatggtcgg ccagttggac gctaccgtcc tcaacattgt 421 ggcgaatctt gaaattggcc ttgatgccgt tcttttgctt gtctgccgta atgtaaacat 481 tatggctgtt gaagttatac tccagtttat ggcccagaat gttaccatcc tctttaaagt 541 cgataccctt caattcgata cgattgacca gcgtgtcacc ttcaaactta acctctgcgc 601 gagttttgta gtagccatca tccttgaagg aaatggtacg ctcctggacg taaccctccg 661 gcatcgcaga tttgaagaaa tcgtgttgtt tcatgtggtc cggataacgc gcaaaacact 721 gcacgccata cgtcaacgtc gtcaccaagg tcggccaagg caccggcagt ttaccggtgg 781 tgcagatgaa cttcagggtc agtttgccgt tggtcgcgtc gccctcacct tcaccgcgaa 841 cgctaaactt atgaccgttc acatcgccgt ccagctcgac cagaatcgga actacgcccg 901 tgaacagttc ttcgccttta cgcatttagt atttctcctc tttctctagt agctagcact 961 atacctagga ctgagctagc cgtaaacacc tgcatatctc ctgactgaga ccgaattcca 1021 gaaatcatcc ttagcgaaag ctaaggattt tttttatctg aaattctgcc tcgtgatacg 1081 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcactcg 1141 agttgatcgg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg 1201 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa 1261 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat 1321 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt 1381 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc 1441 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat 1501 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc 1561 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg 1621 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg 1681 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca 1741 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga 1801 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc 1861 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc 1921 tcgcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc 1981 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcct cgagctgtca 2041 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 2101 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 2161 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 2221 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 2281 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 2341 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 2401 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 2461 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 2521 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 2581 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 2641 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 2701 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 2761 tgatgctcgt caggggggcg gagcctgtgg aaaaacgcca gcaacgcggc ctttttacgg 2821 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 2881 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 2941 gagcgcagcg agtcagtgag cgaggaagcc tgcataacgc //