LOCUS       Exported                5649 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    L3784
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1956..1973)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2020..2048)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2796..2816
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        3394..3498
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3499..4359
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3717..3736)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4530..5118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5019..5038
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5272..5289
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5406..5427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5442..5472
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5480..5496
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5485..5507
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5504..5520
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5504..5520
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt acccggagta gttggcgaag atagaaatgg tgttatctta
       61 tataaattca gactttctct gttaaaattt taaccaccaa aaaatataga atttataaaa
      121 agtttcattt tgaaatttta tcttttgggg ccatttcact accttatcag gaagaaatgt
      181 gtttcatcag aaattgaatt tttcatcttt tcagaaagtg tacctaatac cgtatcatgc
      241 tcatattcac gtgaataagg cttctgccca gttctgtgat tcaaggaaaa ctacgaaaaa
      301 tgatctcatt ttctttgatt tatcaccgag ctggtgtgtc atcacacaca gtctgtttct
      361 tgataaaaac gcgcagaagt tagagcattc attgtgattt tgggtcttta atttctgtaa
      421 aacaaataaa gtgacaagtt tatcatctgc ctattttatc agttatatgc atggttagtt
      481 gcaaataagg gagtagaaat tgactaaaac tcaaaatgag cactgaaacg aggggttttg
      541 ttagttgcga aataacaatt aatacaatct ggttgattac tttgtaaaag gataataaaa
      601 tatgcaaaac ttggcgtatt ctgagatttc attcctaaag acatttatca aaacaaatgt
      661 tttctgcaat tttctaaatt cttggaattc tgcccagcag cctctaatat cgcgttttac
      721 ttagaaaatg acttttggtt tcattttctc atttctggct tctatttttc acaaatgcaa
      781 atattacatt taaattagta ctttcatttc tacaaagatc aaatttaaca ttattgacag
      841 gtttactgtt atcttgtgac agttccgtac tgtcaatcaa aacgaaaatt aaaactcatg
      901 aaatatcaat gaacataaat tgaaaaaaaa acccagttga aaggagaaaa taagcgaaac
      961 acaccaacaa ctgagtagtt acgctcacaa tttggaaatg attttgaaaa ctctctggac
     1021 tgcatttcaa tttttaacaa acaatgaata catactgata gtttttgtac ttctcggcat
     1081 tcagaggcaa cttcctgcca acaacctggc aacttattta gtgccactaa aataagttgc
     1141 caaattgttg gcaggaagtt gcctccaaat gccgagttgg agcaaatgat tcgatatgat
     1201 tttaaggata cctacgcatg cctcctgcca agacacgcac ccacctactg aaatgtcgcg
     1261 gaattttacc tcccaaacta tagattgggt gaaacttcac tgtaaaagct ttttttaagt
     1321 agtaggctgg cagtaatgaa gacgtccata ccataatttt taatgttgta tagaaaatct
     1381 gaattctcaa gtataacatt ttccaataat tagcatagtc agaaactctc caccgccgtt
     1441 ccgttccttt tttttgtaac tatggagtaa tagaacaaga ttattcaaat tcaaattttt
     1501 ataaaacttt acaacgtcct ttccatatat tcttcacaca acatttgaaa aaacaacaaa
     1561 ttcattcgaa atgcattgcc cataatgaat cgaccgaaaa acacaagtga ccgtcaggag
     1621 atcgatagag agagcccgtt ccaactagac aactttttag tgcttatcct tacacacact
     1681 ttctagcttc ataagaaatg catctattat cgtcaccatt tggagacacc agttttagcg
     1741 cacattaata atcgatcagg gtctagagac tcctgttgga ttggcatgcc acgcctacat
     1801 gacatctggc tcttgacggg tgatttgtta cagtgagtag ccacaatttc gcgcattttc
     1861 ttctaaattt cactgttatt tgttcttctg atttatttca tttgaactac ggaaatcgga
     1921 tcctatcgat tcgcggccgc tgtacacccg ggtgccctat agtgagtcgt attggtaccg
     1981 gtagaaaaaa tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     2041 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
     2101 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
     2161 gtaagtttaa acatatatat actaactaac cctgattatt taaattttca gccaacactt
     2221 gtcactactt tctgttatgg tgttcaatgc ttctcgagat acccagatca tatgaaacag
     2281 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     2341 aaagatgacg ggaactacaa gacacgtaag tttaaacagt tcggtactaa ctaaccatac
     2401 atatttaaat tttcaggtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
     2461 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
     2521 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
     2581 gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca gaacttcaaa
     2641 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     2701 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     2761 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     2821 gctgggatta cacatggcat ggacgaacta tacaaaagcc cacaagcttt ggaattcgct
     2881 agccggccat acaagtaatc cggatgatcg acgccaacgt cgttgaattt tcaaatttta
     2941 aatactgaat atttgttttt tttcctatta tttatttatt ctctttgtgt tttttttctt
     3001 gctttctaaa aaattaattc aatccaaatc taaacatttt tttttctctt tccgtctccc
     3061 aattcgtatt ccgctcctct catctgaaca caatgtgcaa gtttatttat cttctcgctt
     3121 tcatttcatt aggacgtggg gggaattggt ggaaggggga aacacacaaa aggatgatgg
     3181 aaatgaaata aggacacaca atatgcaaca acattcaatt cagaaatatg gaggaaggtt
     3241 taaaagaaaa cataaaaata tatagaggag gaaggaaaac tagtaaaaaa taagcaaaga
     3301 aattaggcga acgatgagaa ttgtcctcgc ttgggcccaa aaggcctttt ttttttggcg
     3361 ccgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3421 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3481 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3541 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3601 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3661 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3721 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3781 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3841 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3901 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3961 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4021 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4081 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4141 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4201 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4261 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4321 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4381 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4441 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4501 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4561 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4621 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4681 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4741 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4801 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4861 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4921 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4981 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5041 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5101 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5161 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5221 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5281 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     5341 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     5401 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     5461 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     5521 catgattacg ccaagctgta agtttaaaca tgatcttact aactaactat tctcatttaa
     5581 attttcagag cttaaaaatg gctgaaatca ctcacaacga tggatacgct aacaacttgg
     5641 aaatgaaat
//
