LOCUS Exported 2267 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Provides the End-to-End linker 4-Delta which assists Level 2 cloning in Mobius Assembly. ACCESSION . VERSION . KEYWORDS pMA_Auxiliary 4-Delta SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2267) AUTHORS Andreou AI, Nakayama N TITLE Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly. JOURNAL PLoS One. 2018 Jan 2;13(1):e0189892. doi: 10.1371/journal.pone.0189892. eCollection 2018. PUBMED 29293531 REFERENCE 2 (bases 1 to 2267) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2267 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..58 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(82..99) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(253..841) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(333..352) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1024..1118 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1139..1954) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 1862..1881 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 2116..2134 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2138..2181 /label=bacterial terminator /note="putative bacterial transcription terminator" ORIGIN 1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt 61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc 301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct 1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg 1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt 1141 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac 1201 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata 1261 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta 1321 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg 1381 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc 1441 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg 1501 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat 1561 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt 1621 cttctaatac ctggaatgct gttttccctg ggatcgcagt ggtgagtaac catgcatcat 1681 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta 1741 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca 1801 actctggcgc atcgggcttc ccatacaatc ggtagattgt cgcacctgat tgcccgacat 1861 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc 1921 ttgagcaaga cgtttcccgt tgaatatggc tcattttagc ttccttagct cctgaaaatc 1981 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac 2041 ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg 2101 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta 2161 gctttcgcta aggatgattt ctggaattcc acctgcatat cgctagagac cttctgtatg 2221 aggtggatgg tgtgctgcgt ggccaaaccc atatgcaggt gctgcag //