LOCUS Exported 4707 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Mitochondria, Excitation: 440, Emission: 620. ACCESSION . VERSION . KEYWORDS mKeima-Red-Mito-7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4707) TITLE Davidson Red fluorescent proteins REFERENCE 2 (bases 1 to 4707) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4707 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 537..611 /codon_start=1 /product="mitochondrial presequence of human cytochrome c oxidase subunit VIII (Rizutto et al., 1989)" /label=COX8 presequence /translation="MSVLTPLLLRGLTGSARRLPVPRAK" regulatory 639..648 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 645..1313 /codon_start=1 /product="monomeric Keima-Red fluorescent protein, also known as mKeima" /label=mKeima-Red /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG " polyA_signal 1435..1556 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1472..1491) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1526..1545 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1563..2018) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1700..1721) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1912..1931 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2045..2149 /gene="bla" /label=AmpR promoter promoter 2151..2508 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2174..2194) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2359..2494 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2421..2440 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2543..3337 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2597..2616) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3207..3226 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3525..3544) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3569..3616 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3945..4533 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4434..4453 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgt 541 ccgtcctgac gccgctgctg ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc 601 cgcgcgccaa gatccattcg ttgggggatc caccggtcgc caccatggtg agtgtgatcg 661 ctaaacaaat gacctacaag gtttatatgt caggcacggt caatggacac tactttgagg 721 tcgaaggcga tggaaaagga aagccttacg agggagagca gacagtaaag ctcactgtca 781 ccaagggtgg acctctgcca tttgcttggg atattttatc accacagctt cagtacggaa 841 gcataccatt caccaagtac cctgaagaca tccctgatta tttcaagcag tcattccctg 901 agggatatac atgggagagg agcatgaact ttgaagatgg tgcagtgtgt actgtcagca 961 atgattccag catccaaggc aactgtttca tctacaatgt caaaatctct ggtgagaact 1021 ttcctcccaa tggacctgtt atgcagaaga agacacaggg ctgggaaccc agcactgagc 1081 gtctctttgc acgagatgga atgctgatag gaaacgatta tatggctctg aagttggaag 1141 gaggtggtca ctatttgtgt gaatttaaat ctacttacaa ggcaaagaag cctgtgagga 1201 tgccagggcg ccacgagatt gaccgcaaac tggatgtaac cagtcacaac agggattaca 1261 catctgttga gcagtgtgaa atagccattg cacgccactc tttgctcggt taagcggccg 1321 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac 1381 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg 1441 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 1501 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc 1561 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 1621 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 1681 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 1741 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 1801 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 1861 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 1921 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 1981 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa 2041 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 2101 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga 2161 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 2221 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 2281 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 2341 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 2401 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 2461 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga 2521 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 2581 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 2641 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 2701 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac 2761 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 2821 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 2881 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 2941 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 3001 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 3061 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 3121 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 3181 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 3241 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 3301 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 3361 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 3421 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 3481 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag 3541 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt 3601 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc 3661 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc 3721 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata 3781 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 3841 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 3901 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 3961 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 4021 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 4081 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 4141 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 4201 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 4261 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 4321 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 4381 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 4441 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 4501 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 4561 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 4621 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt 4681 agttcatagc ccatatatgg agttccg //