LOCUS       Exported                8082 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCI-SEP GluR2(R)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Kopec CD, Li B, Wei W, Boehm J, Malinow R
  TITLE     Glutamate receptor exocytosis and spine enlargement during 
            chemically induced long-term potentiation.
  JOURNAL   J Neurosci. 2006 Feb 15. 26(7):2000-9.
  PUBMED    16481433
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1201..1212
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1343..2053
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1370..1398)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1993..2013
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    5196..5317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5207..5226)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5261..5280
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5498..5953
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5585..5604)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5795..5816
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6020..6039)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6139..6161
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6199..6217)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6285..6389
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6390..7250
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6608..6627)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7421..8009
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7910..7929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagtgga tcccccgggc tgcaggaatt
     1081 cggcacgagc tcggctcccc ttccaagaga aacaagagaa acctcacgga aggaagggag
     1141 gaaggaaaga agcaagcaag gaactgcagg aagaaaagag ccggcagagc atcaagaaga
     1201 atcgaaggga ggggagggaa gaccaaatct atggttggac cagggcttct ttttcgccaa
     1261 tgtaaaaagg aatatgccgt acatctttgc ctttttctgc accggttttc taggtgcggt
     1321 tgtgggtgcc aatttcccta ggagtaaagg agaagaactt ttcactggag ttgtcccaat
     1381 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     1441 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     1501 tgttccttgg ccaacacttg tcactacttt aacttatggt gttcaatgct tttcaagata
     1561 cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1621 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     1681 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     1741 aaacattctt ggacacaaat tggaatacaa ctataacgat caccaggtgt acatcatggc
     1801 agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt agacacaaca ttgaagatgg
     1861 aggcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg ggcccgtcct
     1921 tttaccagac aaccattacc tgtttacaac ttctactctt tcgaaagatc ccaacgaaaa
     1981 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     2041 tgaactatac aaagctagca acagcataca gatagggggg ctatttccaa ggggcgctga
     2101 tcaagaatac agtgcatttc gggtagggat ggttcagttt tccacttcgg agttcagact
     2161 gacaccccat atcgacaatt tggaggtagc caacagtttc gcagtcacca atgctttctg
     2221 ctcccagttt tcaagaggag tctacgcaat ttttggattt tatgacaaga agtctgtaaa
     2281 taccatcaca tcattctgtg ggacactcca tgtgtccttc atcacaccta gcttcccaac
     2341 agatggcaca catccatttg tcatccagat gcgacctgac ctcaaaggag cactccttag
     2401 cttgattgag tactaccaat gggacaagtt cgcatacctc tatgacagtg acagaggctt
     2461 atcaacactg caagctgttc tggattctgc tgcagagaag aagtggcagg tgactgctat
     2521 caatgtgggg aacatcaaca atgacaagaa agatgagacc tacagatcgc tctttcaaga
     2581 tctggagtta aaaaaagaac ggcgtgtaat cctggactgt gaaagggata aagtaaatga
     2641 cattgtggac caggttatta ccattggaaa acatgttaaa gggtaccatt atatcattgc
     2701 aaatctggga ttcactgatg gggacctgct gaaaattcag tttggaggag caaatgtctc
     2761 tggatttcag attgtagact acgacgattc cctggtgtct aaatttatag aaagatggtc
     2821 aacactggaa gagaaagaat accctggagc acacacagcg acaattaagt atacttcggc
     2881 cctgacgtat gatgctgtcc aagtgatgac tgaagcattc cgtaaccttc ggaagcagag
     2941 gattgaaata tcccggagag gaaatgcagg ggattgtttg gccaacccag ctgtgccctg
     3001 gggacaaggg gtcgaaatag aaagggccct caagcaggtt caagttgaag gcctctctgg
     3061 aaatataaag tttgaccaga atggaaaacg aataaactac acaattaaca tcatggagct
     3121 caaaacaaat ggaccccgga agattgggta ctggagtgaa gtggataaaa tggttgtcac
     3181 cctaactgag ctcccatcag gaaatgacac gtctgggctt gaaaacaaaa ctgtggtggt
     3241 caccacaata ttggaatctc catatgttat gatgaagaaa aatcatgaaa tgcttgaagg
     3301 gaatgagcgt tacgagggct actgtgttga cttagctgca gaaattgcca aacactgtgg
     3361 gttcaagtac aagctgacta ttgttgggga tggcaagtat ggggccaggg atgccgacac
     3421 caaaatttgg aatggtatgg ttggagagct tgtctacggg aaagctgaca ttgcaattgc
     3481 tccattaact atcactctcg tgagagaaga ggtgattgac ttctccaagc ccttcatgag
     3541 tcttggaatc tctatcatga tcaagaagcc tcagaagtcc aaaccaggag tgttttcctt
     3601 tcttgatcct ttagcctatg agatctggat gtgcattgtg tttgcctaca ttggggtcag
     3661 tgtagtttta ttcctggtca gcagatttag cccctacgag tggcacactg aggaatttga
     3721 agatggaaga gaaacacaaa gtagtgaatc aactaatgaa tttgggattt ttaatagtct
     3781 ctggttttcc ttgggtgcct ttatgcggca gggatgcgat atttcgccaa gatccctctc
     3841 tgggcgcatt gttggaggtg tgtggtggtt ctttaccctg atcataatct cctcctacac
     3901 ggctaactta gctgccttcc tgactgtaga gaggatggtg tctcccatcg aaagtgctga
     3961 ggatctgtct aagcaaacag aaattgctta tggaacatta gactctggct ccactaaaga
     4021 gtttttcagg agatctaaaa tcgcagtgtt tgataaaatg tggacttata tgaggagtgc
     4081 agagccctct gtgtttgtga ggactaccgc agaaggagta gccagagtcc ggaaatccaa
     4141 aggaaagtat gcctacttgc tggagtccac aatgaacgag tacatcgagc agaggaagcc
     4201 ttgtgacacc atgaaagtgg gaggaaactt ggattccaaa ggctacggca tcgccacacc
     4261 taaaggatcc tcattaggaa atgcggttaa cctcgcagta ctaaaactga atgaacaagg
     4321 cctgttggac aaattgaaaa acaaatggtg gtacgacaaa ggagagtgcg gcagcggggg
     4381 aggtgattcc aaggaaaaga ccagtgccct cagtctgagc aacgttgctg gagtattcta
     4441 catccttgtc gggggccttg gtttggcaat gctggtggct ttgattgagt tctgttacaa
     4501 gtcaagggcc gaggcgaaac gaatgaaggt ggcaaagaat ccacagaata ttaacccatc
     4561 ttcctcgcag aattcccaga attttgcaac ttataaggaa ggttacaacg tatatggcat
     4621 cgagagtgtt aaaatttagg ggatgacctt gagtgatgtc atgaggagca aggcaaggct
     4681 gtcaattaca ggaagtactg gagaaaatgg acgtgttatg actccagaat ttcccaaagc
     4741 agtgcatgca ggccttgggc ccaatgatcc gaccagcaaa actcgatgta cttccgagga
     4801 actgatgtgc ataatgcatc aggctggtac attagatccc cgcttaccgc gggcaatata
     4861 gcaacactaa aaactcgatg tacttccgag gaagcgcagt gcataatgct gcgcagtgtt
     4921 gccacataac cactatatta accatttatc tagcggacgc caaaaactca atgtatttct
     4981 gaggaagcgt ggtgcataat gccacgcagc gtctgcataa cttttattat ttcttttatt
     5041 aatcaacaaa attttgtttt taacatttca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     5101 aaaaagggga attcctcgat aattaagcgg ccgctcgaga attcacgcgt ggtacctcta
     5161 gagtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     5221 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     5281 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     5341 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
     5401 atgtggtaaa atcgataagg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg
     5461 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     5521 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5581 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5641 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5701 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5761 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5821 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5881 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5941 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     6001 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     6061 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6121 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6181 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6241 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6301 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6361 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6421 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6481 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6541 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6601 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6661 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6721 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6781 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6841 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6901 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6961 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7021 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7081 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7141 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7201 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7261 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7321 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7381 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7441 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7501 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7561 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7621 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7681 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7741 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7801 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7861 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7921 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7981 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8041 tggccttttg ctggcctttt gctcacatgg ctcgacagat ct
//
