LOCUS       Exported                8171 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Mammalian expression of human histone deacetylase 7 with flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    HDAC7 Flag
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Fischle W, Emiliani S, Hendzel MJ, Nagase T, Nomura N, Voelter W, 
            Verdin E
  TITLE     A new family of human histone deacetylases related to Saccharomyces 
            cerevisiae HDA1p.
  JOURNAL   J Biol Chem. 1999 Apr 23. 274(17):11713-20.
  PUBMED    10206986
REFERENCE   2  (bases 1 to 8171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             2757..2780
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2815..2832)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2821..3045
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3091..3519
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3178..3197)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3388..3409
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3528..3548)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3533..3862
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3713..3848
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3775..3794
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3929..4723
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3983..4002)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4593..4612
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4897..5018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4934..4953)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4988..5007
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5067..5083)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5067..5083)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5080..5102)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5091..5107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5115..5145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5160..5181
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5298..5315)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5469..6057)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5549..5568)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6228..7088)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6851..6870
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7089..7193)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7268..7287)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7459..7838
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7839..8042
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7993..8013
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8087..8106
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8087..8105
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tggacctgcg ggtgggccag cggcccccag tggagccccc accagagccc acattgctgg
       61 ccctgcagcg tccccagcgc ctgcaccacc acctcttcct agcaggcctg cagcagcagc
      121 gctcggtgga gcccatgagg ctctccatgg acacgccgat gcccgagttg caggtgggac
      181 cccaggaaca agagctgcgg cagcttctcc acaaggacaa gagcaagcga agtgctgtag
      241 ccagcagcgt ggtcaagcag aagctagcgg aggtgattct gaaaaaacag caggcggccc
      301 tagaaagaac agtccatccc aacagccccg gcattcccta cagaaccctg gagcccctgg
      361 agacggaagg agccacccgc tccatgctca gcagcttttt gcctcctgtt cccagcctgc
      421 ccagtgaccc cccagagcac ttccctctgc gcaagacagt ctctgagccc aacctgaagc
      481 tgcgctataa gcccaagaag tccctggagc ggaggaagaa tccactgctc cgaaaggaga
      541 gtgcgccccc cagcctccgg cggcggcccg cagagaccct cggagactcc tccccaagta
      601 gtagcagcac gcccgcatca gggtgcagct cccccaatga cagcgagcac ggccccaatc
      661 ccatcctggg ctcggaggct gacagtgacc gcaggaccca tccgactctg ggccctcggg
      721 ggccaatcct ggggagcccc cacactcccc tcttcctgcc ccatggcttg gagcccgagg
      781 ctgggggcac cttgccctct cgcctgcagc ccattctcct cctggacccc tcaggctctc
      841 atgccccgct gctgactgtg cccgggcttg ggcccttgcc cttccacttt gcccagtcct
      901 taatgaccac cgagcggctc tctgggtcag gcctccactg gccactgagc cggactcgct
      961 cagagcccct gccccccagt gccaccgctc ccccaccgcc gggccccatg cagccccgcc
     1021 tggagcagct caaaactcac gtccaggtga tcaagaggtc agccaagccg agtgagaagc
     1081 cccggctgcg gcagataccc tcggctgaag acctggagac agatggcggg ggaccgggcc
     1141 aggtggtgga cgatggcctg gagcacaggg agctgggcca tgggcagcct gaggccagag
     1201 gccccgctcc tctccagcag caccctcagg tgttgctctg ggaacagcag cgactggctg
     1261 ggcggctccc ccggggcagc accggggaca ctgtgctgct tcctctggcc cagggtgggc
     1321 accggcctct gtcccgggct cagtcttccc cagccgcacc tgcctcactg tcagccccag
     1381 agcctgccag ccaggcccga gtcctctcca gctcagagac ccctgccagg accctgccct
     1441 tcaccacagg gctgatctat gactcggtca tgctgaagca ccagtgctcc tgcggtgaca
     1501 acagcaggca cccggagcac gccggccgca tccagagcat ctggtcccgg ctgcaggagc
     1561 gggggctccg gagccagtgt gagtgtctcc gaggccggaa ggcctccctg gaagagctgc
     1621 agtcggtcca ctctgagcgg cacgtgctcc tctacggcac caacccgctc agccgcctca
     1681 aactggacaa cgggaagctg gcagggctcc tggcacagcg gatgtttgtg atgctgccct
     1741 gtggtggggt tggggtggac actgacacca tctggaatga gcttcattcc tccaatgcag
     1801 cccgctgggc cgctggcagt gtcactgacc tcgccttcaa agtggcttct cgtgagctaa
     1861 agaatggttt cgctgtggtg cggcccccag gacaccatgc agatcattca acagccatgg
     1921 gcttctgctt cttcaactca gtggccatcg cctgccggca gctgcaacag cagagcaagg
     1981 ccagcaagat cctcattgta gactgggacg tgcaccatgg caacggcacc cagcaaacct
     2041 tctaccaaga ccccagtgtg ctctacatct ccctgcatcg ccatgacgac ggcaacttct
     2101 tcccggggag tggggctgtg gatgaggtag gggctggcag cggtgagggc ttcaatgtca
     2161 atgtggcctg ggctggaggt ctggaccccc ccatggggga tcctgagtac ctggctgctt
     2221 tcaggatagt cgtgatgccc atcgcccgag agttctctcc agacctagtc ctggtgtctg
     2281 ctggatttga tgctgctgag ggtcacccgg ccccactggg tggctaccat gtttctgcca
     2341 aatgttttgg atacatgacg cagcaactga tgaacctggc aggaggcgca gtggtgctgg
     2401 ccttggaggg tggccatgac ctcacagcca tctgtgacgc ctctgaggcc tgtgtggctg
     2461 ctcttctggg taacagggtg gatccccttt cagaagaagg ctggaaacag aaacccaacc
     2521 tcaatgccat ccgctctctg gaggccgtga tccgggtgca cagtaaatac tggggctgca
     2581 tgcagcgcct ggcctcctgt ccagactcct gggtgcctag agtgccaggg gctgacaaag
     2641 aagaagtgga ggcagtgacc gcactggcgt ccctctctgt gggcatcctg gctgaagata
     2701 ggccctcgga gcagctggtg gaggaggaag aacctatgaa tctcgaattc ggaggagact
     2761 acaaggacga cgatgacaag taatctagag ggcccgttta aacccgctga tcagcctcga
     2821 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     2881 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     2941 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     3001 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     3061 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     3121 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3181 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3241 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3301 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3361 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3421 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3481 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     3541 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3601 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3661 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3721 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3781 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     3841 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     3901 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     3961 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     4021 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     4081 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     4141 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     4201 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     4261 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     4321 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     4381 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     4441 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     4501 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     4561 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     4621 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     4681 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     4741 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     4801 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     4861 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     4921 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4981 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     5041 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5101 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5161 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5221 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5281 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5341 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5401 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5461 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5521 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5581 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5641 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5701 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5761 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5821 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5881 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5941 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6001 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6061 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6121 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6181 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6241 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6301 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6361 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6421 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6481 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6541 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6601 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6661 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6721 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6781 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6841 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6901 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6961 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7021 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7081 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7141 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7201 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     7261 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     7321 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     7381 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     7441 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     7501 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     7561 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     7621 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     7681 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     7741 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     7801 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     7861 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     7921 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     7981 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     8041 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
     8101 atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgagc tcggatccga
     8161 attccagccc a
//
