LOCUS Exported 5377 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION C-terminal part of the split-Cre System. ACCESSION . VERSION . KEYWORDS pCMV-Tag3B-CCre SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5377) AUTHORS Hirrlinger J, Scheller A, Hirrlinger PG, Kellert B, Tang W, Wehr MC, Goebbels S, Reichenbach A, Sprengel R, Rossner MJ, Kirchhoff F TITLE Split-cre complementation indicates coincident activity of different genes in vivo. JOURNAL PLoS One. 2009;4(1):e4286. doi: 10.1371/journal.pone.0004286. Epub 2009 Jan 27. PUBMED 19172189 REFERENCE 2 (bases 1 to 5377) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5377 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 67..370 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 371..574 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 525..545 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" promoter 620..638 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" regulatory 670..679 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 679..708 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 793..849 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 922..942 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" primer_bind complement(1806..1822) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1807..1823) /label=pBluescriptKS /note="For pBluescript vector" primer_bind complement(1878..1897) /label=T7 /note="T7 promoter, forward primer" promoter complement(1879..1897) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 2171..2292 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2208..2227) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2262..2281 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2299..2754) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2436..2457) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2648..2667 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2781..2885 /gene="bla" /label=AmpR promoter promoter 2887..3244 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2910..2930) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3095..3230 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3157..3176 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3279..4073 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3333..3352) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3943..3962 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4261..4280) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4305..4352 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4681..5269 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5170..5189 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc 661 gcggtggcgg ccgccatgga gcagaaactc atctctgaag aggatctgag cccgggcgga 721 tccaacactg aagccgccag gcgttctcgt gcgagaaagt tgcaaagaat gaaacaactt 781 gaagacaagg ttgaagaatt gctttcgaaa aattatcact tggaaaatga ggttgccaga 841 ttaaagaaat tagttggcga acgcgccagc cctagcaacc ctggcgccag caacggcagc 901 ctgcaggaat tcagcggccc tcccaagaag aagaggaagg tgaacaggaa atggttccct 961 gctgaacctg aggatgtgag ggactacctc ctgtacctgc aagccagagg cctggctgtg 1021 aagaccatcc aacagcacct gggccagctc aacatgctgc acaggagatc tggcctgcct 1081 cgcccttctg actccaatgc tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg 1141 gatgctgggg agagagccaa gcaggccctg gcctttgaac gcactgactt tgaccaagtc 1201 agatccctga tggagaactc tgacagatgc caggacatca ggaacctggc cttcctgggc 1261 attgcctaca acaccctgct gcgcattgcc gaaattgcca gaatcagagt gaaggacatc 1321 tcccgcaccg atggtgggag aatgctgatc cacattggca ggaccaagac cctggtgtcc 1381 acagctggtg tggagaaggc cctgtccctg ggggttacca agctggtgga gagatggatc 1441 tctgtgtctg gtgtggctga tgaccccaac aactacctgt tctgccgggt cagaaagaat 1501 ggtgtggctg ccccttctgc cacctcccaa ctgtccaccc gggccctgga agggatcttt 1561 gaggccaccc accgcctgat ctatggtgcc aaggatgact ctgggcagag atacctggcc 1621 tggtctggcc actctgccag agtgggtgct gccagggaca tggccagggc tggtgtgtcc 1681 atccctgaaa tcatgcaggc tggtggctgg accaatgtga acattgtgat gaactacatc 1741 agaaacctgg actctgagac tggggccatg gtgaggctgc tcgaggatgg ggactagaag 1801 cttatcgata ccgtcgacct cgaggggggg cccggtacct taattaatta aggtaccagg 1861 taagtgtacc caattcgccc tatagtgagt cgtattacaa ttcactcgat cgcccttccc 1921 aacagttgcg cagcctgaat ggcgaatgga gatccaattt ttaagtgtat aatgtgttaa 1981 actactgatt ctaattgttt gtgtatttta gattcacagt cccaaggctc atttcaggcc 2041 cctcagtcct cacagtctgt tcatgatcat aatcagccat accacatttg tagaggtttt 2101 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 2161 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 2221 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 2281 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt 2341 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 2401 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 2461 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 2521 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 2581 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 2641 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 2701 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg 2761 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 2821 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 2881 agaatcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc 2941 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 3001 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3061 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 3121 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 3181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 3241 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 3301 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 3361 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 3421 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat 3481 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 3541 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 3601 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 3661 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 3721 tggaagccgg tcttgtcgat caggatgatc tggacgaaga acatcagggg ctcgcgccag 3781 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc 3841 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 3901 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 3961 ttgctgaaga acttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 4021 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 4081 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 4141 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 4201 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac 4261 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca 4321 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg 4381 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt 4441 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc 4501 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca 4561 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 4621 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 4681 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 4741 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 4801 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 4861 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 4921 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 4981 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 5041 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 5101 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 5161 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 5221 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 5281 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 5341 gttatcccct gattctgtgg ataaccgtat taccgcc //