LOCUS Exported 11244 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions. ACCESSION . VERSION . KEYWORDS pRVL-3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11244) AUTHORS Vazquez-Lombardi R, Nevoltris D, Rouet R, Christ D TITLE Expression of IgG Monoclonals with Engineered Immune Effector Functions. JOURNAL Methods Mol Biol. 2018;1827:313-334. doi: 10.1007/978-1-4939-8648-4_16. PUBMED 30196504 REFERENCE 2 (bases 1 to 11244) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11244 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 4..383 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 384..587 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 538..558 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 584..608 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 629..638 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 1728..1862 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1752..1771) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1806..1825 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(2005..2027) /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 2587..4376 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" CDS complement(4678..6603) /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" primer_bind complement(7038..7056) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7124..7228 /gene="bla" /label=AmpR promoter CDS 7229..8089 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7447..7466) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8260..8848 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8749..8768 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 9279..9424 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" CDS 9467..10504 /codon_start=1 /product="hygromycin B phosphotransferase" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE MGEAN" primer_bind complement(10502..10521) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 10546..10594 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" ORIGIN 1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat 421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc 541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc 601 gtcagatctc tagaagctgg gtaccgccgc caccatgggc tggagctgca tcatcctgtt 661 cctggtggcc accgccaccg gcgtgcacag cgaagagcta cagagtgagc tcttcgccaa 721 gaccaccgcc cccagcgtgt accccctggc ccccgtgtgc ggcggcacca ccggcagcag 781 cgtgaccctg ggctgcctgg tgaagggcta cttccccgag cccgtgaccc tgacctggaa 841 cagcggcagc ctgagcagcg gcgtgcacac cttccccgcc ctgctgcaga gcggcctgta 901 caccctgagc agcagcgtga ccgtgaccag caacacctgg cccagccaga ccatcacctg 961 caacgtggcc caccccgcca gcagcaccaa ggtggacaag aagatcgagc ctagggtgcc 1021 catcacccag aacccctgcc cccctctgaa ggagtgccca ccctgcgccg cccccgacgc 1081 agagggcgct cccagcgtgt tcatcttccc ccccaagatc aaggacgtgc tgatgatcag 1141 cctgagcccc atggtgacct gcgtggtggt ggacgtgagc gaggacgacc ccgacgtgca 1201 gatcagctgg ttcgtgaaca acgtggaggt gcacaccgcc cagacccaga cccacaggga 1261 ggactacaac agcaccctga gggtggtgag cgccctgccc atccagcacc aggactggat 1321 gagcggcaag gccttcgcat gcgctgtgaa caacagggcc ctgccaagcc ctatcgagaa 1381 gaccatcagc aagcccaggg gccccgtgag ggccccccag gtgtacgtgc tgcctccccc 1441 agccgaggag atgaccaaga aggagttcag cctgacctgc atgatcaccg gcttcctgcc 1501 cgccgagatc gccgtggact ggaccagcaa cggcaggacc gagcagaact acaagaacac 1561 cgccaccgtg ctggacagcg acggcagcta cttcatgtac agcaagctga gggtgcagaa 1621 aagcacctgg gagaggggca gcctgttcgc ctgcagcgtg gtgcacgagg gcctgcacaa 1681 ccacctgacc accaagacca tcagcaggag cctgggcaag tgataaggat ccagacatga 1741 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta 1801 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 1861 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt 1921 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg gctgcctcgc 1981 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 2041 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 2101 cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat cgatccccgc cccggacgaa 2161 ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt aatcccttca 2221 gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg gggggaccaa 2281 acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc acatttgcca 2341 acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca acacaacatt 2401 gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc 2461 caggggccca ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc 2521 taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta 2581 accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta 2641 attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag 2701 ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta tttacatggg 2761 gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa 2821 ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct tcgtttagct 2881 aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt 2941 ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc attgtgctat 3001 gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag gaatgaaaca 3061 ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa agactggatg 3121 tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa tatgatactg 3181 gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt gttacactct 3241 atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact ggttgtctct 3301 aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca aagacaagtg 3361 gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac acgccgccct 3421 gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc ccgctaacca 3481 ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat acctgcataa 3541 gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca aattacacac 3601 acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag gtcgctgaga 3661 gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg gatagcatat 3721 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat 3781 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag 3841 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat 3901 gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagtatat 3961 gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata gcatatgcta 4021 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gcataggcta 4081 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta 4141 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta 4201 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta 4261 tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt gctatccttt 4321 gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt ttcctgctgg 4381 ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca tgtctgattg 4441 ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc gcggagctga 4501 gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa aatggtgaca 4561 agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga tttattcttt 4621 agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca agttacatca 4681 ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat ctccgtcatc 4741 accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat ctactccatc 4801 gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg tcataacaag 4861 gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa agaccatgaa 4921 ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag gggccattcc 4981 aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg cagttcctcg 5041 ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg cgacccaagt 5101 tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta aaccttctgc 5161 aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa accaccctcc 5221 ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc cttctcctgg 5281 gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac catctgggcc 5341 accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa atggccttct 5401 acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac tgggactcct 5461 gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac ttccccccct 5521 ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc ggcctccact 5581 acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc ctgctcctgc 5641 ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc 5701 tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc 5761 tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc 5821 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc 5881 tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgcccctc ctgcccctcc 5941 tcctgctcct gcccctcctg ctcctgcccc tcctgcccct cctgcccctc ctgctcctgc 6001 ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc 6061 tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc 6121 tcctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc 6181 ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc 6241 tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc 6301 tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt ccaccgtggg tccctttgca 6361 gccaatgcaa cttggacgtt tttggggtct ccggacacca tctctatgtc ttggccctga 6421 tcctgagccg cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc 6481 tcgtccatgg ttatcacccc ctcttctttg aggtccactg ccgccggagc cttctggtcc 6541 agatgtgtct cccttctctc ctaggccatt tccaggtcct gtacctggcc cctcgtcaga 6601 catgattcac actaaaagag atcaatagac atctttatta gacgacgctc agtgaataca 6661 gggagtgcag actcctgccc cctccaacag cccccccacc ctcatcccct tcatggtcgc 6721 tgtcagacag atccaggtct gaaaattccc catcctccga accatcctcg tcctcatcac 6781 caattactcg cagcccggaa aactcccgct gaacatcctc aagatttgcg tcctgagcct 6841 caagccaggc ctcaaattcc tcgtccccct ttttgctgga cggtagggat ggggattctc 6901 gggacccctc ctcttcctct tcaaggtcac cagacagaga tgctactggg gcaacggaag 6961 aaaagctggg tgcggcctgt gaggatcagc ttatcgatga taagctgtca aacatgagaa 7021 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 7081 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 7141 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 7201 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 7261 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 7321 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 7381 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 7441 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 7501 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 7561 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 7621 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 7681 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 7741 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact 7801 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 7861 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 7921 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 7981 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 8041 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 8101 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 8161 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 8221 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 8281 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 8341 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 8401 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 8461 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 8521 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 8581 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 8641 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 8701 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 8761 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 8821 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 8881 ggccttttgc tggccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat 8941 ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc 9001 catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccccgagatg 9061 cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt 9121 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg 9181 ttagcgaggt gccgccctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt 9241 ccccggaaga aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg 9301 tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca 9361 cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc 9421 ttaacagcgt caacagcgtg ccgcagatcc cggggggcaa tgagatatga aaaagcctga 9481 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct 9541 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg 9601 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg 9661 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga 9721 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga 9781 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc 9841 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac 9901 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt 9961 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc 10021 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct 10081 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc 10141 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca 10201 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta 10261 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga 10321 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg 10381 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact 10441 cgccgatagt ggaaaccgac gccccagcac tcgtccggat cgggagatgg gggaggctaa 10501 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac 10561 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc 10621 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc 10681 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg 10741 gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga 10801 atggtttatg gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact 10861 ggactgagca gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc 10921 gcgacacgaa caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc 10981 gcggatttct ggcgtgccaa gctagtcgac caattctcat gtttgacagc ttatcatcgc 11041 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct 11101 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca 11161 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg 11221 gctcatgtcc aatatgaccg ccat //