LOCUS       Exported               10262 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Mammalian expression vector for catalytically inactive Cpf1 from 
            Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator.
ACCESSION   .
VERSION     .
KEYWORDS    JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Tak YE, Kleinstiver BP, Nunez JK, Hsu JY, Horng JE, Gong J, Weissman
            JS, Joung JK
  TITLE     Inducible and multiplex gene regulation using CRISPR-Cpf1-based 
            transcription factors.
  JOURNAL   Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483.
  PUBMED    29083402
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..663
                     /label=chicken beta-actin promoter
     intron          664..1680
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1604..1626
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1688..1707
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1779..5462
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             5463..5510
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5517..5597
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5628..5777
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5802..5822
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6252..6608
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             6627..7196
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     primer_bind     complement(7283..7302)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7348..7403
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7402..7421)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7764..7780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7764..7780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7777..7799)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7788..7804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7812..7842)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7857..7878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7937..8132
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7983..8118
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8045..8064
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    8138..8272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8175..8194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8229..8248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8340..8357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8511..9099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8591..8610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9270..10130)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9893..9912
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10131..10235)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
      541 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
      601 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
      661 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
      721 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
      781 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
      841 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
      961 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     1021 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     1081 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     1141 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     1201 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     1261 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt
     1741 cgacggtacc gcgggcccgg gatccaccgg tcgccaccat gagcaagctg gagaagttta
     1801 caaactgcta ctccctgtct aagaccctga ggttcaaggc catccctgtg ggcaagaccc
     1861 aggagaacat cgacaataag cggctgctgg tggaggacga gaagagagcc gaggattata
     1921 agggcgtgaa gaagctgctg gatcgctact atctgtcttt tatcaacgac gtgctgcaca
     1981 gcatcaagct gaagaatctg aacaattaca tcagcctgtt ccggaagaaa accagaaccg
     2041 agaaggagaa taaggagctg gagaacctgg agatcaatct gcggaaggag atcgccaagg
     2101 ccttcaaggg caacgagggc tacaagtccc tgtttaagaa ggatatcatc gagacaatcc
     2161 tgccagagtt cctggacgat aaggacgaga tcgccctggt gaacagcttc aatggcttta
     2221 ccacagcctt caccggcttc tttgataaca gagagaatat gttttccgag gaggccaaga
     2281 gcacatccat cgccttcagg tgtatcaacg agaatctgac ccgctacatc tctaatatgg
     2341 acatcttcga gaaggtggac gccatctttg ataagcacga ggtgcaggag atcaaggaga
     2401 agatcctgaa cagcgactat gatgtggagg atttctttga gggcgagttc tttaactttg
     2461 tgctgacaca ggagggcatc gacgtgtata acgccatcat cggcggcttc gtgaccgaga
     2521 gcggcgagaa gatcaagggc ctgaacgagt acatcaacct gtataatcag aaaaccaagc
     2581 agaagctgcc taagtttaag ccactgtata agcaggtgct gagcgatcgg gagtctctga
     2641 gcttctacgg cgagggctat acatccgatg aggaggtgct ggaggtgttt agaaacaccc
     2701 tgaacaagaa cagcgagatc ttcagctcca tcaagaagct ggagaagctg ttcaagaatt
     2761 ttgacgagta ctctagcgcc ggcatctttg tgaagaacgg ccccgccatc agcacaatct
     2821 ccaaggatat cttcggcgag tggaacgtga tccgggacaa gtggaatgcc gagtatgacg
     2881 atatccacct gaagaagaag gccgtggtga ccgagaagta cgaggacgat cggagaaagt
     2941 ccttcaagaa gatcggctcc ttttctctgg agcagctgca ggagtacgcc gacgccgatc
     3001 tgtctgtggt ggagaagctg aaggagatca tcatccagaa ggtggatgag atctacaagg
     3061 tgtatggctc ctctgagaag ctgttcgacg ccgattttgt gctggagaag agcctgaaga
     3121 agaacgacgc cgtggtggcc atcatgaagg acctgctgga ttctgtgaag agcttcgaga
     3181 attacatcaa ggccttcttt ggcgagggca aggagacaaa cagggacgag tccttctatg
     3241 gcgattttgt gctggcctac gacatcctgc tgaaggtgga ccacatctac gatgccatcc
     3301 gcaattatgt gacccagaag ccctactcta aggataagtt caagctgtat tttcagaacc
     3361 ctcagttcat gggcggctgg gacaaggata aggagacaga ctatcgggcc accatcctga
     3421 gatacggctc caagtactat ctggccatca tggataagaa gtacgccaag tgcctgcaga
     3481 agatcgacaa ggacgatgtg aacggcaatt acgagaagat caactataag ctgctgcccg
     3541 gccctaataa gatgctgcca aaggtgttct tttctaagaa gtggatggcc tactataacc
     3601 ccagcgagga catccagaag atctacaaga atggcacatt caagaagggc gatatgttta
     3661 acctgaatga ctgtcacaag ctgatcgact tctttaagga tagcatctcc cggtatccaa
     3721 agtggtccaa tgcctacgat ttcaactttt ctgagacaga gaagtataag gacatcgccg
     3781 gcttttacag agaggtggag gagcagggct ataaggtgag cttcgagtct gccagcaaga
     3841 aggaggtgga taagctggtg gaggagggca agctgtatat gttccagatc tataacaagg
     3901 acttttccga taagtctcac ggcacaccca atctgcacac catgtacttc aagctgctgt
     3961 ttgacgagaa caatcacgga cagatcaggc tgagcggagg agcagagctg ttcatgaggc
     4021 gcgcctccct gaagaaggag gagctggtgg tgcacccagc caactcccct atcgccaaca
     4081 agaatccaga taatcccaag aaaaccacaa ccctgtccta cgacgtgtat aaggataaga
     4141 ggttttctga ggaccagtac gagctgcaca tcccaatcgc catcaataag tgccccaaga
     4201 acatcttcaa gatcaataca gaggtgcgcg tgctgctgaa gcacgacgat aacccctatg
     4261 tgatcggcat cgccaggggc gagcgcaatc tgctgtatat cgtggtggtg gacggcaagg
     4321 gcaacatcgt ggagcagtat tccctgaacg agatcatcaa caacttcaac ggcatcagga
     4381 tcaagacaga ttaccactct ctgctggaca agaaggagaa ggagaggttc gaggcccgcc
     4441 agaactggac ctccatcgag aatatcaagg agctgaaggc cggctatatc tctcaggtgg
     4501 tgcacaagat ctgcgagctg gtggagaagt acgatgccgt gatcgccctg gaggacctga
     4561 actctggctt taagaatagc cgcgtgaagg tggagaagca ggtgtatcag aagttcgaga
     4621 agatgctgat cgataagctg aactacatgg tggacaagaa gtctaatcct tgtgcaacag
     4681 gcggcgccct gaagggctat cagatcacca ataagttcga gagctttaag tccatgtcta
     4741 cccagaacgg cttcatcttt tacatccctg cctggctgac atccaagatc gatccatcta
     4801 ccggctttgt gaacctgctg aaaaccaagt ataccagcat cgccgattcc aagaagttca
     4861 tcagctcctt tgacaggatc atgtacgtgc ccgaggagga tctgttcgag tttgccctgg
     4921 actataagaa cttctctcgc acagacgccg attacatcaa gaagtggaag ctgtactcct
     4981 acggcaaccg gatcagaatc ttccggaatc ctaagaagaa caacgtgttc gactgggagg
     5041 aggtgtgcct gaccagcgcc tataaggagc tgttcaacaa gtacggcatc aattatcagc
     5101 agggcgatat cagagccctg ctgtgcgagc agtccgacaa ggccttctac tctagcttta
     5161 tggccctgat gagcctgatg ctgcagatgc ggaacagcat cacaggccgc accgacgtgg
     5221 attttctgat cagccctgtg aagaactccg acggcatctt ctacgatagc cggaactatg
     5281 aggcccagga gaatgccatc ctgccaaaga acgccgacgc caatggcgcc tataacatcg
     5341 ccagaaaggt gctgtgggcc atcggccagt tcaagaaggc cgaggacgag aagctggata
     5401 aggtgaagat cgccatctct aacaaggagt ggctggagta cgcccagacc agcgtgaagc
     5461 acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctacc
     5521 catacgatgt tccagattac gcttatccct acgacgtgcc tgattatgca tacccatatg
     5581 atgtccccga ctatgccgga agcgaggcca gcggttccgg acgggctgac gcattggacg
     5641 attttgatct ggatatgctg ggaagtgacg ccctcgatga ttttgacctt gacatgcttg
     5701 gttcggatgc ccttgatgac tttgacctcg acatgctcgg cagtgacgcc cttgatgatt
     5761 tcgacctgga catgctgatt aactctagaa gttccggatc tccgaaaaag aaacgcaaag
     5821 ttggtagcca gtacctgccc gacaccgacg accggcaccg gatcgaggaa aagcggaagc
     5881 ggacctacga gacattcaag agcatcatga agaagtcccc cttcagcggc cccaccgacc
     5941 ctagacctcc acctagaaga atcgccgtgc ccagcagatc cagcgccagc gtgccaaaac
     6001 ctgcccccca gccttacccc ttcaccagca gcctgagcac catcaactac gacgagttcc
     6061 ctaccatggt gttccccagc ggccagatct ctcaggcctc tgctctggct ccagcccctc
     6121 ctcaggtgct gcctcaggct cctgctcctg caccagctcc agccatggtg tctgcactgg
     6181 ctcaggcacc agcacccgtg cctgtgctgg ctcctggacc tccacaggct gtggctccac
     6241 cagcccctaa acctacacag gccggcgagg gcacactgtc tgaagctctg ctgcagctgc
     6301 agttcgacga cgaggatctg ggagccctgc tgggaaacag caccgatcct gccgtgttca
     6361 ccgacctggc cagcgtggac aacagcgagt tccagcagct gctgaaccag ggcatccctg
     6421 tggcccctca caccaccgag cccatgctga tggaataccc cgaggccatc acccggctcg
     6481 tgacaggcgc tcagaggcct cctgatccag ctcctgcccc tctgggagca ccaggcctgc
     6541 ctaatggact gctgtctggc gacgaggact tcagctctat cgccgatatg gatttctcag
     6601 ccttgctggg ctctggcagc ggcagccggg attccaggga agggatgttt ttgccgaagc
     6661 ctgaggccgg ctccgctatt agtgacgtgt ttgagggccg cgaggtgtgc cagccaaaac
     6721 gaatccggcc atttcatcct ccaggaagtc catgggccaa ccgcccactc cccgccagcc
     6781 tcgcaccaac accaaccggt ccagtacatg agccagtcgg gtcactgacc ccggcaccag
     6841 tccctcagcc actggatcca gcgcccgcag tgactcccga ggccagtcac ctgttggagg
     6901 atcccgatga agagacgagc caggctgtca aagcccttcg ggagatggcc gatactgtga
     6961 ttccccagaa ggaagaggct gcaatctgtg gccaaatgga cctttcccat ccgcccccaa
     7021 ggggccatct ggatgagctg acaaccacac ttgagtccat gaccgaggat ctgaacctgg
     7081 actcacccct gaccccggaa ttgaacgaga ttctggatac cttcctgaac gacgagtgcc
     7141 tcttgcatgc catgcatatc agcacaggac tgtccatctt cgacacatct ctgttttaag
     7201 gccgcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     7261 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     7321 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     7381 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     7441 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     7501 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     7561 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     7621 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     7681 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     7741 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7801 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7861 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7921 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7981 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8041 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8101 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
     8161 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8221 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     8281 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8341 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8401 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8461 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8521 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8581 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8641 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8701 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8761 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8821 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8881 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8941 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9001 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9061 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9121 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9181 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9241 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9301 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9361 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9421 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9481 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9541 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9601 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9661 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9721 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9781 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9841 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9901 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9961 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10021 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10081 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10141 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10201 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10261 gg
//
