LOCUS Exported 190 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Expresses GFP from internal ribosome entry sequence, cloning component. ACCESSION . VERSION . KEYWORDS pcDNA3.1(+)IRES GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 190) AUTHORS Schaefer MR, Williams M, Kulpa DA, Blakely PK, Yaffee AQ, Collins KL TITLE A novel trafficking signal within the HLA-C cytoplasmic tail allows regulated expression upon differentiation of macrophages. JOURNAL J Immunol. 2008 Jun 15;180(12):7804-17. PUBMED 18523244 REFERENCE 2 (bases 1 to 190) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..190 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 24..43 /label=T7 /note="T7 promoter, forward primer" promoter 24..42 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag 61 cgtttaaact taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg 121 cagatatcca gcacagtggc ggccgctcga ggttaacgaa ttccgccccc cccccccccc 181 cccccctcac //