LOCUS Exported 4004 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses ShadowY in mammalian cells. ACCESSION . VERSION . KEYWORDS CMV-ShadowY SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4004) AUTHORS Murakoshi H, Shibata ACE TITLE ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement. JOURNAL Sci Rep. 2017 Jul 28;7(1):6791. doi: 10.1038/s41598-017-07002-4. PUBMED 28754922 REFERENCE 2 (bases 1 to 4004) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4004 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 547..556 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(598..619) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(859..878) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1206..1227 /label=EGFP-C /note="EGFP, forward primer" misc_feature 1270..1335 /label=MCS /note="multiple cloning site" polyA_signal 1460..1581 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1497..1516) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1551..1570 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 1588..2043 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1675..1694) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1885..1906 /label=F1ori-F /note="F1 origin, forward primer" promoter 2099..2203 /gene="bla" /label=AmpR promoter CDS 2204..3064 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2422..2441) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3235..3823 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3724..3743 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 661 gatgccacct acggcaagct gaccctgaag ctcatctgca ccaccggcaa gctgcccgtg 721 ccctggccca ccctcgtgac caccttcggc tacggcctga tgtgcttcgc gcgctatcca 781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 961 atcctggggc acaagctcga gtacgcttgg cccgtggtga atgtctatat catggccgac 1021 aagcagaaga acggcatcaa ggtgaacttc tctatctatc ataacatcga ggacggcagc 1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacgggcc cgtgctgctg 1141 cccgacaacc actacctgag ctactcggct aagctgagca aagaccccaa cgagaagcgc 1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1261 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc 1321 gcgggcccgg gatcctaagc ggccgcgaca ctagatcata atcagccata ccacatttgt 1381 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 1441 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 1501 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 1561 caaactcatc aatgtatctt aacgcgtacg cgccctgtag cggcgcatta agcgcggcgg 1621 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 1681 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 1741 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 1801 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 1861 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 1921 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 1981 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa 2041 tttcctctag ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 2101 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 2161 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 2221 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 2281 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 2341 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 2401 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 2461 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 2521 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 2581 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 2641 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 2701 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 2761 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaactgat 2821 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 2881 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 2941 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 3001 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 3061 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 3121 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 3181 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 3241 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 3301 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 3361 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 3421 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 3481 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 3541 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 3601 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 3661 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 3721 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 3781 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 3841 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggctcgac agatcccctg 3901 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa 3961 tcaattacgg ggtcattagt tcatagccca tatatggagt tccg //