LOCUS Exported 15174 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses dCas9-YY1. ACCESSION . VERSION . KEYWORDS pAW90.dCas9-YY1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15174) AUTHORS Weintraub AS, Li CH, Zamudio AV, Sigova AA, Hannett NM, Day DS, Abraham BJ, Cohen MA, Nabet B, Buckley DL, Guo YE, Hnisz D, Jaenisch R, Bradner JE, Gray NS, Young RA TITLE YY1 Is a Structural Regulator of Enhancer-Promoter Loops. JOURNAL Cell. 2017 Dec 14;171(7):1573-1588.e28. doi: 10.1016/j.cell.2017.11.008. Epub 2017 Dec 7. PUBMED 29224777 REFERENCE 2 (bases 1 to 15174) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15174 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2687..3865 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2918..3856 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3813..3833 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 3881..3928 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 3929..8029 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the N863A mutation in the HNH catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 8084..8104 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 8333..8362 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 8336..8365 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 9389..9442 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 9443..9841 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_feature 9866..10454 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(9919..9939) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(10337..10348) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(10457..10473) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10458..10474) /label=pBluescriptKS /note="For pBluescript vector" LTR 10979..11159 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(11185..11202) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 11191..11415 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 11461..11889 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(11548..11567) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 11758..11779 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(11898..11918) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 11903..12232 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 12083..12218 /label=SV40 ori /note="SV40 origin of replication" primer_bind 12145..12164 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 12280..12327 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 12346..12720 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 12850..12971 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(12887..12906) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 12941..12960 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(13020..13036) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(13020..13036) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(13033..13055) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 13044..13060 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(13068..13098) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 13113..13134 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(13251..13268) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(13422..14010) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(13502..13521) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(14181..15041) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 14804..14823 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(15042..15146) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa 2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg 2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa 2761 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg 2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa 2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg 2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct 3001 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc 3061 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg 3121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct 3181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt 3241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg 3301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag 3361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc 3421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga 3481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg 3541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc 3601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt 3661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac 3721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt 3781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt 3841 ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg aaaaggccgg cggccacgaa 3901 aaaggccggc caggcaaaaa agaaaaagga caagaagtac agcatcggcc tggccatcgg 3961 caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt 4021 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct 4081 gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata 4141 caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa 4201 ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa 4261 gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta 4321 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg 4381 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg 4441 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta 4501 caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct 4561 gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga 4621 gaagaagaat ggcctgttcg gcaacctgat tgccctgagc ctgggcctga cccccaactt 4681 caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga 4741 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc 4801 cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat 4861 caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct 4921 gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt 4981 cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt 5041 ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa 5101 gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca 5161 ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt 5221 cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt 5281 gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac 5341 catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat 5401 cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag 5461 cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga 5521 gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct 5581 gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat 5641 cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg 5701 cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa 5761 cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat 5821 cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa 5881 gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga 5941 caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa 6001 cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca 6061 ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc 6121 cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg 6181 ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa 6241 gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg 6301 cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta 6361 cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg 6421 gctgtccgac tacgatgtgg accacatcgt gcctcagagc tttctgaagg acgactccat 6481 cgacaacaag gtgctgacca gaagcgacaa ggcccggggc aagagcgaca acgtgccctc 6541 cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat 6601 tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga 6661 taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc 6721 acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga 6781 agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt 6841 ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt 6901 cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga 6961 ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc 7021 taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct 7081 ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat 7141 cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt 7201 gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc 7261 caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg 7321 cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg 7381 caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag 7441 cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa 7501 ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag 7561 aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata 7621 tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa 7681 tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca 7741 gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc 7801 cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct 7861 gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga 7921 ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat 7981 caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca gcgctggagg 8041 aggtggaagc ggaggaggag gaagcggagg aggaggtagc ggacctaaga aaaagaggaa 8101 ggtggcggcc gctggatccg gacgggctgc ctcgggcgac accctctaca tcgccacgga 8161 cggctcggag atgccggccg agatcgtgga gctgcacgag atcgaggtgg agaccatccc 8221 ggtggagacc atcgagacca cagtggtggg cgaggaggag gaggaggacg acgacgacga 8281 ggacggcggc ggtggcgacc acggcggcgg gggcggccac gggcacgccg gccaccacca 8341 ccaccaccat caccaccacc accacccgcc catgatcgct ctgcagccgc tggtcaccga 8401 cgacccgacc caggtgcacc accaccagga ggtgatcctg gtgcagacgc gcgaggaggt 8461 ggtgggcggc gacgactcgg acgggctgcg cgccgaggac ggcttcgagg atcagattct 8521 catcccggtg cccgcgccgg ccggcggcga cgacgactac attgaacaaa cgctggtcac 8581 cgtggcggcg gccggcaaga gcggcggcgg cggctcgtcg tcgtcgggag gcggccgcgt 8641 caagaagggc ggcggcaaga agagcggcaa gaagagttac ctcagcggcg gggccggcgc 8701 ggcgggcggc ggcggcgccg acccgggcaa caagaagtgg gagcagaagc aggtgcagat 8761 caagaccctg gagggcgagt tctcggtcac catgtggtcc tcagatgaaa aaaaagatat 8821 tgaccatgag acagtggttg aagaacagat cattggagag aactcacctc ctgattattc 8881 agaatatatg acaggaaaga aacttcctcc tggaggaata cctggcattg acctctcaga 8941 tcccaaacaa ctggcagaat ttgctagaat gaagccaaga aaaattaaag aagatgatgc 9001 tccaagaaca atagcttgcc ctcataaagg ctgcacaaag atgttcaggg ataactcggc 9061 catgagaaaa catctgcaca cccacggtcc cagagtccac gtctgtgcag aatgtggcaa 9121 agcttttgtt gagagttcaa aactaaaacg acaccaactg gttcatactg gagagaagcc 9181 ctttcagtgc acgttcgaag gctgtgggaa acgcttttca ctggacttca atttgcgcac 9241 acatgtgcga atccataccg gagacaggcc ctatgtgtgc cccttcgatg gttgtaataa 9301 gaagtttgct cagtcaacta acctgaaatc tcacatctta acacatgcta aggccaaaaa 9361 caaccagatt aactgtacag gcagtggaga gggcagagga agtctgctaa catgcggtga 9421 cgtcgaggag aatcctggcc caatggccaa gcctttgtct caagaagaat ccaccctcat 9481 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag 9541 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg 9601 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct 9661 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg 9721 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg 9781 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta 9841 agaattcgat atcaagctta tcggtaatca acctctggat tacaaaattt gtgaaagatt 9901 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 9961 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 10021 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 10081 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 10141 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 10201 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 10261 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 10321 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 10381 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg 10441 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac 10501 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga 10561 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc 10621 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 10681 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg 10741 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa 10801 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt 10861 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag 10921 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg 10981 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 11041 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 11101 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 11161 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 11221 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 11281 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 11341 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 11401 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 11461 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 11521 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 11581 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 11641 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 11701 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 11761 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 11821 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 11881 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 11941 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 12001 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 12061 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 12121 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 12181 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 12241 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca 12301 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc 12361 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac 12421 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac 12481 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg 12541 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac 12601 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag 12661 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga 12721 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 12781 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 12841 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 12901 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 12961 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 13021 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 13081 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 13141 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 13201 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 13261 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 13321 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 13381 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 13441 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 13501 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 13561 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 13621 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 13681 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 13741 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 13801 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 13861 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 13921 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 13981 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 14041 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 14101 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 14161 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 14221 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 14281 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 14341 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 14401 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 14461 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 14521 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 14581 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 14641 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 14701 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 14761 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 14821 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 14881 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 14941 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 15001 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 15061 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 15121 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac //