LOCUS Exported 5581 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Localization: Gap Junctions, Excitation: 590, Emission: 649. ACCESSION . VERSION . KEYWORDS mPlum-Cx32-7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5581) TITLE Davidson Red fluorescent proteins REFERENCE 2 (bases 1 to 5581) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5581 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 1507..2187 /codon_start=1 /product="far-red monomeric derivative of DsRed fluorescent protein (Wang et al., 2004)" /label=mPlum /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH STGA" primer_bind 1908..1927 /label=mCherry-F /note="mCherry, forward primer" primer_bind 2102..2125 /label=DsRed1-C /note="DsRed1, forward primer" polyA_signal 2309..2430 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2346..2365) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2400..2419 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2437..2892) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2574..2595) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2786..2805 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2919..3023 /gene="bla" /label=AmpR promoter promoter 3025..3382 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3048..3068) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3233..3368 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3295..3314 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3417..4211 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3471..3490) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4081..4100 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4399..4418) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4443..4490 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4819..5407 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5308..5327 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggactcag atctcgagct caagcttcga attccctctg ggaaagggca gcagcagcca 601 ggtgtggcag tgacagggag gtgtgaatga ggcaggatga actggacagg tttgtacacc 661 ttgctcagtg gcgtgaaccg gcattctact gccattggcc gagtatggct ctcggtcatc 721 ttcatcttca gaatcatggt gctggtggtg gctgcagaga gtgtgtgggg tgatgagaaa 781 tcttccttca tctgcaacac actccagcct ggctgcaaca gcgtttgcta tgaccaattc 841 ttccccatct cccatgtgcg gctgtggtcc ctgcagctca tcctagtttc caccccagct 901 ctcctcgtgg ccatgcacgt ggctcaccag caacacatag agaagaaaat gctacggctt 961 gagggccatg gggaccccct acacctggag gaggtgaaga ggcacaaggt ccacatctca 1021 gggacactgt ggtggaccta tgtcatcagc gtggtgttcc ggctgttgtt tgaggccgtc 1081 ttcatgtatg tcttttatct gctctaccct ggctatgcca tggtgcggct ggtcaagtgc 1141 gacgtctacc cctgccccaa cacagtggac tgcttcgtgt cccgccccac cgagaaaacc 1201 gtcttcaccg tcttcatgct agctgcctct ggcatctgca tcatcctcaa tgtggccgag 1261 gtggtgtacc tcatcatccg ggcctgtgcc cgccgagccc agcgccgctc caatccacct 1321 tcccgcaagg gctcgggctt cggccaccgc ctctcacctg aatacaagca gaatgagatc 1381 aacaagctgc tgagtgagca ggatggctcc ctgaaagaca tactgcgccg cagccctggc 1441 accggggctg ggctggctga aaagagcgac cgctgctcgg cctgcgcgga tccaccggtg 1501 gctaccatgg tgagcaaggg cgaggaggtc atcaaggagt tcatgcgctt caaggagcac 1561 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc 1621 tacgagggca cccagaccgc caggctgaag gtgaccaagg gtggccccct gcccttcgcc 1681 tgggacatcc tgtcccctca gatcatgtac ggctccaagg cctacgtgaa gcaccccgcc 1741 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg 1801 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag 1861 ttcatctaca aggtgaaggt gcgcggcacc aacttcccct ccgacggccc cgtaatgcag 1921 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg 1981 aagggcgaga tgaagatgag gctgaggctg aaggacggcg gccactacga cgccgaggtc 2041 aagaccacct acatggccaa gaagcccgtg cagctgcccg gcgcctacaa gaccgacatc 2101 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgcgcc 2161 gagggccgcc actccaccgg cgcctaagcg gccgcgactc tagatcataa tcagccatac 2221 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa 2281 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa 2341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 2401 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt 2461 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 2521 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 2581 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 2641 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 2701 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 2761 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 2821 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 2881 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 2941 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 3001 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca 3061 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 3121 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 3181 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 3241 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 3301 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 3361 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga 3421 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 3481 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 3541 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag 3601 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 3661 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 3721 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 3781 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 3841 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 3901 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg 3961 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 4021 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 4081 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 4141 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 4201 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 4261 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 4321 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 4381 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat 4441 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg 4501 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata 4561 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct 4621 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta 4681 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 4741 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 4801 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 4861 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 4921 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 4981 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 5041 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 5101 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 5161 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 5221 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 5281 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 5341 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 5401 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 5461 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca 5521 ttagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc 5581 g //