LOCUS Exported 5188 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Nucleus/Centromeres, Excitation: 590, Emission: 649. ACCESSION . VERSION . KEYWORDS mPlum-CENPB-N-22 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5188) AUTHORS Day RN, Davidson MW TITLE The fluorescent protein palette: tools for cellular imaging. JOURNAL Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 2009 Aug 4. PUBMED 19771335 REFERENCE 2 (bases 1 to 5188) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5188 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 535..544 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" misc_feature 1048..1100 /label=MCS /note="multiple cloning site" CDS 1114..1794 /codon_start=1 /product="far-red monomeric derivative of DsRed fluorescent protein (Wang et al., 2004)" /label=mPlum /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH STGA" primer_bind 1515..1534 /label=mCherry-F /note="mCherry, forward primer" primer_bind 1709..1732 /label=DsRed1-C /note="DsRed1, forward primer" polyA_signal 1916..2037 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1953..1972) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2007..2026 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2044..2499) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2181..2202) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2393..2412 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2526..2630 /gene="bla" /label=AmpR promoter promoter 2632..2989 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2655..2675) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2840..2975 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2902..2921 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3024..3818 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3078..3097) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3688..3707 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4006..4025) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4050..4097 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4426..5014 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4915..4934 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc 541 atgggcccca agcggcggca gctgacgttc cgggagaagt cgcggatcat ccaggaggtg 601 gaggagaacc cggacctacg caagggcgag atcgcgcggc gcttcaacat cccgccgtcc 661 acgctgagca cgatcctgaa gaacaagcgc gccatcctgg cgtcggagcg caagtacggg 721 gtggcctcca cctgccgcaa gaccaacaag ctgtctccct acgacaagct cgagggcttg 781 ctcatcgcct ggttccagca gatccgcgcc gccggcctgc cggtcaaggg catcatcctc 841 aaggagaagg cgctgcgcat agccgaggag ctgggcatgg acgacttcac cgcctccaac 901 ggctggctgg accgcttccg ccggcgccac ggcgtggtgt cctgcagcgg cgtggcccgc 961 gcccgcgcgc gaaacgctgc cccccgcacc ccggcggcgc ctgccagtcc ggccgcggtg 1021 ccctcggagg gcagtggcgg gagcactctc gagctcaagc ttcgaattct gcagtcgacg 1081 gtaccgcggg cccgggatcc accggtggct accatggtga gcaagggcga ggaggtcatc 1141 aaggagttca tgcgcttcaa ggagcacatg gagggctccg tgaacggcca cgagttcgag 1201 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccag gctgaaggtg 1261 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagat catgtacggc 1321 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc 1381 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc 1441 caggactcct ccctgcagga cggcgagttc atctacaagg tgaaggtgcg cggcaccaac 1501 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag 1561 cggatgtacc ccgaggacgg cgccctgaag ggcgagatga agatgaggct gaggctgaag 1621 gacggcggcc actacgacgc cgaggtcaag accacctaca tggccaagaa gcccgtgcag 1681 ctgcccggcg cctacaagac cgacatcaag ctggacatca cctcccacaa cgaggactac 1741 accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgc ctaagcggcc 1801 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 1861 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 1921 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 1981 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg 2041 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 2101 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 2161 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 2221 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 2281 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 2341 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 2401 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 2461 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa 2521 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 2581 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg 2641 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 2701 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 2761 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 2821 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 2881 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 2941 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag 3001 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 3061 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 3121 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 3181 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 3241 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 3301 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 3361 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 3421 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 3481 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 3541 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 3601 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 3661 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 3721 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 3781 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 3841 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 3901 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 3961 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga 4021 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg 4081 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac 4141 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc 4201 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat 4261 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 4321 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 4381 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 4441 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 4501 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 4561 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 4621 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 4681 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 4741 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 4801 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 4861 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 4921 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 4981 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 5041 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 5101 tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat 5161 tagttcatag cccatatatg gagttccg //