LOCUS       Exported               11954 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  FUS(1-214) fused to mCh-Cry2olig.
ACCESSION   .
VERSION     .
KEYWORDS    pHR-FUSN-mCh-Cry2olig
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Shin Y, Berry J, Pannucci N, Haataja MP, Toettcher JE, Brangwynne CP
  TITLE     Spatiotemporal Control of Intracellular Phase Transitions Using 
            Light-Activated optoDroplets.
  JOURNAL   Cell. 2017 Jan 12;168(1-2):159-171.e14. doi: 
            10.1016/j.cell.2016.11.054. Epub 2016 Dec 29.
  PUBMED    28041848
REFERENCE   2  (bases 1 to 11954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1175..1186
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2166..3659
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     misc_feature    3811..4399
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3864..3884)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4282..4293)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4402..4418)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4403..4419)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4509..4742
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4767..4785)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4768..4785)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5075..5094)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5308..5327)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5427..5449
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5487..5505)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5573..5677
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5678..6538
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5896..5915)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6709..7297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7198..7217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7451..7468
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7538..7558)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7543..7872
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7723..7858
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7785..7804
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          9006..9071
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9201..9221
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9646..9780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9683..9702)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9737..9756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9948..10581
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10628..10753
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11250..11483
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11668..11712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
      781 gatccggagc taccatggcc tcaaacgatt atacccaaca agcaacccaa agctatgggg
      841 cctaccccac ccagcccggg cagggctatt cccagcagag cagtcagccc tacggacagc
      901 agagttacag tggttatagc cagtccacgg acacttcagg ctatggccag agcagctatt
      961 cttcttatgg ccagagccag aacacaggct atggaactca gtcaactccc cagggatatg
     1021 gctcgactgg cggctatggc agtagccaga gctcccaatc gtcttacggg cagcagtcct
     1081 cctatcctgg ctatggccag cagccagctc ccagcagcac ctcgggaagt tacggtagca
     1141 gttctcagag cagcagctat gggcagcccc agagtgggag ctacagccag cagcctagct
     1201 atggtggaca gcagcaaagc tatggacagc agcaaagcta taatccccct cagggctatg
     1261 gacagcagaa ccagtacaac agcagcagtg gtggtggagg tggaggtgga ggtggaggta
     1321 actatggcca agatcaatcc tccatgagta gtggtggtgg cagtggtggc ggttatggca
     1381 atcaagacca gagtggtgga ggtggcagcg gtggctatgg acagcaggac cgtggaggtg
     1441 gaggaatggt gtctaaaggc gaggaggata acatggctat aatcaaggaa tttatgaggt
     1501 tcaaggtgca tatggaggga tctgtgaacg gtcacgagtt cgaaatcgag ggcgaaggcg
     1561 aggggcgacc ctatgaaggg acacagacgg ccaaactgaa ggtgaccaag ggtggccccc
     1621 tgcctttcgc ctgggacatc ctgtcacccc agtttatgta cggctctaag gcttacgtta
     1681 agcaccctgc cgatattccg gactacttga aactgagctt ccctgaaggg ttcaagtggg
     1741 aacgggtaat gaattttgag gatggcggtg tagtcacagt tacccaggac agctctcttc
     1801 aggacggaga atttatctat aaggttaaac ttcggggcac taacttccca tcagacggcc
     1861 ccgtgatgca gaagaaaact atgggctggg aagccagctc agaacgcatg tatcccgagg
     1921 acggagcact gaagggagaa atcaagcagc gactcaagct gaaggatggg ggacattatg
     1981 atgctgaggt caaaaccacc tataaggcca agaaacccgt tcagcttcct ggggcataca
     2041 acgtgaatat caaactggat attacatccc ataacgaaga ctataccatc gtggaacagt
     2101 acgagcgggc cgagggcaga catagcacag ggggcatgga tgaattgtac aaggcggcca
     2161 cgcgtatgaa gatggacaaa aagactatag tttggtttag aagagaccta aggattgagg
     2221 ataatcctgc attagcagca gctgctcacg aaggatctgt ttttcctgtc ttcatttggt
     2281 gtcctgaaga agaaggacag ttttatcctg gaagagcttc aagatggtgg atgaaacaat
     2341 cacttgctca cttatctcaa tccttgaagg ctcttggatc tgacctcact ttaatcaaaa
     2401 cccacaacac gatttcagcg atcttggatt gtatccgcgt taccggtgct acaaaagtcg
     2461 tctttaacca cctctatgat cctgtttcgt tagttcggga ccataccgta aaggagaagc
     2521 tggtggaacg tgggatctct gtgcaaagct acaatggaga tctattgtat gaaccgtggg
     2581 agatatactg cgaaaagggc aaacctttta cgagtttcaa ttcttactgg aagaaatgct
     2641 tagatatgtc gattgaatcc gttatgcttc ctcctccttg gcggttgatg ccaataactg
     2701 cagcggctga agcgatttgg gcgtgttcga ttgaagaact agggctggag aatgaggccg
     2761 agaaaccgag caatgcgttg ttaactagag cttggtctcc aggatggagc aatgctgata
     2821 agttactaaa tgagttcatc gagaagcagt tgatagatta tgcaaagaac agcaagaaag
     2881 ttgttgggaa ttctacttca ctactttctc cgtatctcca tttcggggaa ataagcgtca
     2941 gacacgtttt ccagtgtgcc cggatgaaac aaattatatg ggcaagagat aagaacagtg
     3001 aaggagaaga aagtgcagat ctttttctta ggggaatcgg tttaagagag tattctcggt
     3061 atatatgttt caacttcccg tttactcacg agcaatcgtt gttgagtcat cttcggtttt
     3121 tcccttggga tgctgatgtt gataagttca aggcctggag acaaggcagg accggttatc
     3181 cgttggtgga tgccggaatg agagagcttt gggctaccgg atggatgcat aacagaataa
     3241 gagtgattgt ttcaagcttt gctgtgaagt ttcttctcct tccatggaaa tggggaatga
     3301 agtatttctg ggatacactt ttggatgctg atttggaatg tgacatcctt ggctggcagt
     3361 atatctctgg gagtatcccc gatggccacg agcttgatcg cttggacaat cccgcgttac
     3421 aaggcgccaa atatgaccca gaaggtgagt acataaggca atggcttccc gagcttgcga
     3481 gattgccaac tgaatggatc catcatccat gggacgctcc tttaaccgta ctcaaagctt
     3541 ctggtgtgga actcggaaca aactatgcga aacccattgt agacatcgac acagctcgtg
     3601 agctactagc taaagctatt tcaagaaccc gtggagcaca gatcatgatc ggagcagcag
     3661 cccgggatcc accggtcgcc accggttcag ggagcggatc cggctcaggg agtgcggccg
     3721 caactcccac ctgcaacatg cgtgactgac tgaggccgcg actctagagt cgacctgcag
     3781 gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     3841 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3901 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3961 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4021 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4081 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4141 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4201 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4261 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4321 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4381 ctttgggccg cctccccgca tcgataccgt cgacctcgag ggaattaatt cgagctcggt
     4441 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     4501 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     4561 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4621 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4681 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4741 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     4801 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     4861 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     4921 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     4981 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     5041 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     5101 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     5161 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     5221 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     5281 ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5341 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5401 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5461 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5521 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5581 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5641 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5701 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5761 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5821 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5881 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5941 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6001 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6061 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6121 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6181 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6241 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6301 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6361 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6421 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6481 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6541 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6601 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6661 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6721 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6781 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6841 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6901 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6961 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7021 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7081 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7141 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7201 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7261 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7321 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7381 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7441 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7501 tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt
     7561 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7621 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7681 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7741 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7801 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7861 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
     7921 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     7981 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
     8041 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
     8101 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
     8161 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
     8221 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
     8281 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
     8341 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
     8401 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
     8461 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
     8521 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
     8581 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
     8641 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
     8701 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
     8761 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
     8821 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
     8881 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
     8941 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
     9001 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     9061 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
     9121 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
     9181 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
     9241 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
     9301 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
     9361 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
     9421 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
     9481 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
     9541 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     9601 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     9661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     9781 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     9841 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
     9901 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
     9961 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
    10021 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
    10081 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
    10141 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
    10201 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
    10261 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
    10321 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
    10381 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    10441 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    10501 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    10561 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
    10621 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
    10681 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
    10741 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
    10801 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
    10861 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
    10921 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
    10981 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
    11041 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
    11101 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
    11161 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    11221 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    11281 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    11341 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    11401 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    11461 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    11521 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    11581 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    11641 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    11701 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    11761 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    11821 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
    11881 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
    11941 attcgattag tgaa
//
