LOCUS       Exported                2333 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway entry vector for N-term FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    pENTR4-FLAG (w210-2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      114..157
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      289..316
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     324..347
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    366..465
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             474..497
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(570..669)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(781..800)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             792..1601
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(859..878)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1691..2279
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2180..2199
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgctagcatg
       61 gatctcgggg acgtctaact actaagcgag agtagggaac tgccaggcat caaataaaac
      121 gaaaggccca gtcttccgac tgagcctttc gttttatctg ttgtttgtcg gtgaacgctc
      181 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa cggcccggag
      241 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aactaagcag aaggccatcc
      301 tgacggatgg cctttttgcg tttctacaaa ctcttcctgt tagttagtta cttaagctcg
      361 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata
      421 agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctccacc atggattaca
      481 aggacgatga cgacaaactc ggatccatgg gaaccaattc agtcgactgg atccggtacc
      541 gaattcgcgg ccgcactcga gatatctaga cccagctttc ttgtacaaag ttggcattat
      601 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
      661 cattatttgc catccagctg cagctctggc ccgtgtctca aaatctctga tgttacattg
      721 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
      781 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
      841 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
      901 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
      961 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     1021 tgcctcttcc gaccatcaag cattttatcc gtactcctgg tgatgcatgg ttactcacca
     1081 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     1141 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     1201 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     1261 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     1321 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     1381 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     1441 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     1501 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     1561 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     1621 tgtaacatta ttcagattgg gccccgttcc actgagcgtc agaccccgta gaaaagatca
     1681 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1741 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1801 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1861 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1921 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1981 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2041 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2101 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2161 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2221 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2281 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gct
//
