LOCUS       Exported               12115 bp ds-DNA     circular SYN 14-FEB-2022
DEFINITION  Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21.
ACCESSION   .
VERSION     .
KEYWORDS    pSMPP-mCherry-hTRIM21
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12115)
  AUTHORS   Clift D, McEwan WA, Labzin LI, Konieczny V, Mogessie B, James LC, 
            Schuh M
  TITLE     A Method for the Acute and Rapid Degradation of Endogenous Proteins.
  JOURNAL   Cell. 2017 Nov 13. pii: S0092-8674(17)31255-2. doi: 
            10.1016/j.cell.2017.10.033.
  PUBMED    29153837
REFERENCE   2  (bases 1 to 12115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 14, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12115
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    141..275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(178..197)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     232..251
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             443..1076
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1123..1248
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1745..1978
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2163..2207
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2492..2609
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2762..3169
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3178..3187
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3184..3891
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3329..3347)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3597..3616
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      3898..3907
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(5444..5464)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5862..5873)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5905..6404
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5940..5963)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6337..6356
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6425..7024
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6425..6444)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6921..6941
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7119..7352
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7377..7395)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7378..7395)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7685..7704)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7918..7937)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8037..8059
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8097..8115)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8183..8287
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8288..9148
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8506..8525)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9319..9907
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9808..9827
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10061..10078
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10148..10168)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10153..10482
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10333..10468
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10395..10414
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          11616..11681
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11811..11831
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
       61 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
      121 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
      181 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
      241 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
      301 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
      361 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
      421 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
      481 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
      541 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
      601 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
      661 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
      721 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
      781 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
      841 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
      901 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      961 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     1021 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     1081 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     1141 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     1201 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     1261 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     1321 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1381 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1441 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1501 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1561 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
     1621 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1681 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1741 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1801 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1861 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1921 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1981 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     2041 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2101 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2161 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2221 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2281 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2341 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2401 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     2461 ggtatcgcca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     2521 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2581 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     2641 tttggatcga taagcttgat atcgaattcc tgcagccccg ataaaataaa agattttatt
     2701 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctgc
     2761 agtaacgcca ttttgcaagg catggaaaaa taccaaacca agaatagaga agttcagatc
     2821 aagggcgggt acatgaaaat agctaacgtt gggccaaaca ggatatctgc ggtgagcagt
     2881 ttcggccccg gcccggggcc aagaacagat ggtcaccgca gtttcggccc cggcccgagg
     2941 ccaagaacag atggtcccca gatatggccc aaccctcagc agtttcttaa gacccatcag
     3001 atgtttccag gctcccccaa ggacctgaaa tgaccctgcg ccttatttga attaaccaat
     3061 cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc gagctctata aaagagctca
     3121 caacccctca ctcggcgcgc cagtcctccg acagactgag tcgcccgggg gggatccgcc
     3181 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     3241 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     3301 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     3361 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     3421 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     3481 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     3541 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     3601 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     3661 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     3721 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     3781 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     3841 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gacgcgtgcc
     3901 accatggctt cagcagcacg cttgacaatg atgtgggagg aggtcacatg ccctatctgc
     3961 ctggacccct tcgtggagcc tgtgagcatc gagtgtggcc acagcttctg ccaggaatgc
     4021 atctctcagg ttgggaaagg tgggggcagc gtctgtcctg tgtgccggca gcgctttctg
     4081 ctcaagaatc tccggcccaa tcgacagcta gccaacatgg tgaacaacct taaagaaatc
     4141 agccaggagg ccagagaggg cacacagggg gaacggtgtg cagtgcatgg agagagactt
     4201 cacctgttct gtgagaaaga tgggaaggcc ctttgctggg tatgtgccca gtctcggaaa
     4261 caccgtgacc acgccatggt ccctcttgag gaggctgcac aggagtacca ggagaagctc
     4321 caggtggcat taggggaact gagaagaaag caggagttgg ctgagaagtt ggaagtggaa
     4381 attgcaataa agagagcaga ctggaagaaa acagtggaaa cacagaaatc taggattcac
     4441 gcagagtttg tgcagcaaaa aaacttcctg gttgaagaag aacagaggca gctgcaggag
     4501 ctggagaagg atgagaggga gcagctgaga atcctggggg agaaagaggc caagctggcc
     4561 cagcagagcc aggccctaca ggagctcatc tcagagctag atcgaaggtg ccacagctca
     4621 gcactggaac tgctgcagga ggtgataatt gtcctggaaa ggagtgagtc ctggaacctg
     4681 aaggacctgg atattacctc tccagaactc aggagtgtgt gccatgtgcc agggctgaag
     4741 aagatgctga ggacatgtgc agtccacatc actctggatc cagacacagc caatccgtgg
     4801 ctgatacttt cagaagatcg gagacaagtg aggcttggag acacccagca gagcatacct
     4861 ggaaatgaag agagatttga tagttatcct atggtcctgg gtgcccagca ctttcactct
     4921 ggaaaacatt actgggaggt agatgtgaca ggaaaggagg cctgggacct gggtgtctgc
     4981 agagactctg tgcgcaggaa ggggcacttt ttgcttagtt ccaagagtgg cttctggaca
     5041 atttggttgt ggaacaaaca aaaatatgag gctggcacct acccccagac tcccctccac
     5101 cttcaggtgc ctccatgcca agttgggatt ttcctggact atgaggctgg catggtctcc
     5161 ttctacaaca tcactgacca tggctccctc atctactcct tctctgaatg tgcctttaca
     5221 ggacctctgc ggcccttctt cagtcctggt ttcaatgatg gaggaaaaaa cacagcccct
     5281 ctaaccctct gtccactgaa tattggatca caaggatcca ctgactattg agcggccgcg
     5341 actctagagt cgacctgcag gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc
     5401 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5461 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5521 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5581 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5641 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5701 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5761 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     5821 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5881 accttccttc ccatcaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     5941 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     6001 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     6061 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     6121 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     6181 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     6241 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggcnnnggg
     6301 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     6361 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     6421 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     6481 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     6541 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     6601 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     6661 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     6721 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     6781 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     6841 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     6901 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     6961 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     7021 ctgactcgag ggaattaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc
     7081 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7141 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7201 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7261 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7321 acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta
     7381 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
     7441 tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
     7501 tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
     7561 ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc
     7621 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     7681 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     7741 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     7801 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     7861 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac
     7921 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     7981 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     8041 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     8101 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     8161 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8221 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8281 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8341 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8401 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8461 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8521 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8581 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8641 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8701 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8761 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8821 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8881 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8941 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9001 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9061 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9121 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9181 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9241 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9301 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9361 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9421 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9481 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9541 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9601 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9661 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9721 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9781 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9841 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9901 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9961 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    10021 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    10081 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    10141 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
    10201 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
    10261 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
    10321 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    10381 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
    10441 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca
    10501 ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta
    10561 aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc
    10621 gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca
    10681 catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc
    10741 aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct
    10801 gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt
    10861 tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat
    10921 ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt
    10981 cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg
    11041 ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat
    11101 atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg
    11161 ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga
    11221 ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca
    11281 tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc
    11341 ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat
    11401 ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga
    11461 tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg
    11521 gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat
    11581 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
    11641 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
    11701 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
    11761 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
    11821 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
    11881 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
    11941 cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca
    12001 gttataatca taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta
    12061 ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaa
//
