LOCUS Exported 5153 bp ds-DNA circular SYN 25-MAY-2021 DEFINITION IFN-beta promoter driving luciferase. Use in IFN reporter assay.. ACCESSION . VERSION . KEYWORDS IFN-Beta_pGL3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5153) AUTHORS Gentili M, Kowal J, Tkach M, Satoh T, Lahaye X, Conrad C, Boyron M, Lombard B, Durand S, Kroemer G, Loew D, Dalod M, Thery C, Manel N TITLE Transmission of innate immune signaling by packaging of cGAMP in viral particles. JOURNAL Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628. Epub 2015 Jul 30. PUBMED 26229115 REFERENCE 2 (bases 1 to 5153) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5153 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 660..2312 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(726..744) /label=LucNrev /note="Firefly luciferase, reverse primer" polyA_signal 2353..2474 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2364..2383) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2418..2437 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(2722..2739) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2893..3481) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2973..2992) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3652..4512) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4275..4294 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4513..4617) /gene="bla" /label=AmpR promoter rep_origin 4644..5099 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4731..4750) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4941..4962 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 cttcgctatt acgccagaat tctcaggtcg tttgctttcc tttgctttct cccaagtctt 61 gttttacaat ttgctttagt cattcactga aattttaaaa aacattagaa aacctcacag 121 tttgtaaatc tttttcccta ttatatatat cataagatag gagcttaaat aaagagtttt 181 agaaactact aaaatgtaaa tgacatagga aaactgaaag ggagaagtga aagtgggaaa 241 ttcctctgaa tagagagagg accatctcat ataaataggc catacccatg gagaaaggac 301 attctaactg caacctttcg aattctcagg tcgtttgctt tcctttgctt tctcccaagt 361 cttgttttac aatttgcttt agtcattcac tgaaatttta aaaaacatta gaaaacctca 421 cagtttgtaa atctttttcc ctattatata tatcataaga taggagctta aataaagagt 481 tttagaaact actaaaatgt aaatgacata ggaaaactga aagggagaag tgaaagtggg 541 aaattcctct gaatagagag aggaccatct catataaata ggccataccc atggagaaag 601 gacattctaa ctgcaacctt tcgctagccc gggctcgaga tctgcgatct aagtaagcta 661 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa 721 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg 781 cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg 841 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat 901 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg 961 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt 1021 cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa 1081 aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat 1141 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt 1201 ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat 1261 ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc 1321 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg 1381 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc 1441 gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca 1501 agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga 1561 ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta 1621 aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg 1681 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg 1741 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa 1801 cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt 1861 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg 1921 gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc 1981 tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac 2041 accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc 2101 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt 2161 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg 2221 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa 2281 aggccaagaa gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc 2341 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa 2401 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct 2461 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg 2521 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc 2581 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 2641 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg 2701 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 2761 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 2821 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 2881 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 2941 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 3001 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 3061 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 3121 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 3181 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 3241 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 3301 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 3361 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 3421 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 3481 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 3541 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 3601 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 3661 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 3721 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 3781 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 3841 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 3901 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 3961 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 4021 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 4081 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 4141 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 4201 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 4261 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 4321 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 4381 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 4441 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 4501 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 4561 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 4621 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 4681 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 4741 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 4801 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 4861 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 4921 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 4981 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 5041 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg 5101 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cct //