LOCUS Exported 11205 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter. ACCESSION . VERSION . KEYWORDS pTDH3-dCas9-Mxi1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11205) AUTHORS Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman JS, Qi LS TITLE CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. JOURNAL Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. PUBMED 23849981 REFERENCE 2 (bases 1 to 11205) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11205 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 30..52 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 44..61 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 45..61 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 68..87 /label=T7 /note="T7 promoter, forward primer" promoter 68..86 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 136..802 /gene="S. cerevisiae TDH3" /label=GAP promoter /note="promoter for glyceraldehyde-3-phosphate dehydrogenase" primer_bind 672..695 /label=GPDpro-F /note="S. cerevisiae GPD promoter, forward primer" regulatory 810..819 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 825..845 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4962..4982 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5016..5138 /codon_start=1 /product="transcriptional repressor domain from the mouse Max-interacting protein 1 (Schreiber-Agus et al., 1995)" /label=Mxi1 /translation="MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR" terminator 5151..5338 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(5206..5230) /label=pHybLex-R /note="ADH terminator, reverse primer" promoter complement(5415..5433) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5454..5470) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5454..5470) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5467..5489) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 5478..5494 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5502..5532) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5547..5568 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5685..5702) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5856..6444) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5936..5955) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6615..7475) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7238..7257 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7476..7580) /gene="bla" /label=AmpR promoter misc_feature 7617..8120 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" primer_bind 8161..8179 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(8217..8239) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 8339..8358 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" CDS complement(8852..9946) /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /label=LEU2 /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" promoter complement(9947..10354) /gene="S. cerevisiae LEU2" /label=LEU2 promoter rep_origin complement(10654..11109) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(10791..10812) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 11003..11022 /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 61 tgaattgtaa tacgactcac tatagggcga attggagctc actcggaatt cagtctacta 121 gtcagttcga gtttatcatt atcaatactg ccatttcaaa gaatacgtaa ataattaata 181 gtagtgattt tcctaacttt atttagtcaa aaaattagcc ttttaattct gctgtaaccc 241 gtacatgccc aaaatagggg gcgggttaca cagaatatat aacatcgtag gtgtctgggt 301 gaacagttta ttcctggcat ccactaaata taatggagcc cgctttttaa gctggcatcc 361 agaaaaaaaa agaatcccag caccaaaata ttgttttctt caccaaccat cagttcatag 421 gtccattctc ttagcgcaac tacagagaac aggggcacaa acaggcaaaa aacgggcaca 481 acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg acacaaggca attgacccac 541 gcatgtatct atctcatttt cttacacctt ctattacctt ctgctctctc tgatttggaa 601 aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta ttcccctact tgactaataa 661 gtatataaag acggtaggta ttgattgtaa ttctgtaaat ctatttctta aacttcttaa 721 attctacttt tatagttagt ctttttttta gttttaaaac accaagaact tagtttcgaa 781 taaacacaca taaacaaaca aaacccgccg ccaccatgga cgtcccaaag aagaagagaa 841 aggtaggcag cgacaagaag tattctatcg gactggccat cgggactaat agcgtcgggt 901 gggccgtgat cactgacgag tacaaggtgc cctctaagaa gttcaaggtg ctcgggaaca 961 ccgaccggca ttccatcaag aaaaatctga tcggagctct cctctttgat tcaggggaga 1021 ccgctgaagc aacccgcctc aagcggactg ctagacggcg gtacaccagg aggaagaacc 1081 ggatttgtta ccttcaagag atattctcca acgaaatggc aaaggtcgac gacagcttct 1141 tccataggct ggaagaatca ttcctcgtgg aagaggataa gaagcatgaa cggcatccca 1201 tcttcggtaa tatcgtcgac gaggtggcct atcacgagaa atacccaacc atctaccatc 1261 ttcgcaaaaa gctggtggac tcaaccgaca aggcagacct ccggcttatc tacctggccc 1321 tggcccacat gatcaagttc agaggccact tcctgatcga gggcgacctc aatcctgaca 1381 atagcgatgt ggataaactg ttcatccagc tggtgcagac ttacaaccag ctctttgaag 1441 agaaccccat caatgcaagc ggagtcgatg ccaaggccat tctgtcagcc cggctgtcaa 1501 agagccgcag acttgagaat cttatcgctc agctgccggg tgaaaagaaa aatggactgt 1561 tcgggaacct gattgctctt tcacttgggc tgactcccaa tttcaagtct aatttcgacc 1621 tggcagagga tgccaagctg caactgtcca aggacaccta tgatgacgat ctcgacaacc 1681 tcctggccca gatcggtgac caatacgccg accttttcct tgctgctaag aatctttctg 1741 acgccatcct gctgtctgac attctccgcg tgaacactga aatcaccaag gcccctcttt 1801 cagcttcaat gattaagcgg tatgatgagc accaccagga cctgaccctg cttaaggcac 1861 tcgtccggca gcagcttccg gagaagtaca aggaaatctt ctttgaccag tcaaagaatg 1921 gatacgccgg ctacatcgac ggaggtgcct cccaagagga attttataag tttatcaaac 1981 ctatccttga gaagatggac ggcaccgaag agctcctcgt gaaactgaat cgggaggatc 2041 tgctgcggaa gcagcgcact ttcgacaatg ggagcattcc ccaccagatc catcttgggg 2101 agcttcacgc catccttcgg cgccaagagg acttctaccc ctttcttaag gacaacaggg 2161 agaagattga gaaaattctc actttccgca tcccctacta cgtgggaccc ctcgccagag 2221 gaaatagccg gtttgcttgg atgaccagaa agtcagaaga aactatcact ccctggaact 2281 tcgaagaggt ggtggacaag ggagccagcg ctcagtcatt catcgaacgg atgactaact 2341 tcgataagaa cctccccaat gagaaggtcc tgccgaaaca ttccctgctc tacgagtact 2401 ttaccgtgta caacgagctg accaaggtga aatatgtcac cgaagggatg aggaagcccg 2461 cattcctgtc aggcgaacaa aagaaggcaa ttgtggacct tctgttcaag accaatagaa 2521 aggtgaccgt gaagcagctg aaggaggact atttcaagaa aattgaatgc ttcgactctg 2581 tggagattag cggggtcgaa gatcggttca acgcaagcct gggtacctac catgatctgc 2641 ttaagatcat caaggacaag gattttctgg acaatgagga gaaagaggac atccttgagg 2701 acattgtcct gactctcact ctgttcgagg accgggaaat gatcgaggag aggcttaaga 2761 cctacgccca tctgttcgac gataaagtga tgaagcaact taaacggaga agatataccg 2821 gatggggacg ccttagccgc aaactcatca acggaatccg ggacaaacag agcggaaaga 2881 ccattcttga tttccttaag agcgacggat tcgctaatcg caacttcatg caacttatcc 2941 atgatgattc cctgaccttt aaggaggaca tccagaaggc ccaagtgtct ggacaaggtg 3001 actcactgca cgagcatatc gcaaatctgg ctggttcacc cgctattaag aagggtattc 3061 tccagaccgt gaaagtcgtg gacgagctgg tcaaggtgat gggtcgccat aaaccagaga 3121 acattgtcat cgagatggcc agggaaaacc agactaccca gaagggacag aagaacagca 3181 gggagcggat gaaaagaatt gaggaaggga ttaaggagct cgggtcacag atccttaaag 3241 agcacccggt ggaaaacacc cagcttcaga atgagaagct ctatctgtac taccttcaaa 3301 atggacgcga tatgtatgtg gaccaagagc ttgatatcaa caggctctca gactacgacg 3361 tggacgccat cgtccctcag agcttcctca aagacgactc aattgacaat aaggtgctga 3421 ctcgctcaga caagaaccgg ggaaagtcag ataacgtgcc ctcagaggaa gtcgtgaaaa 3481 agatgaagaa ctattggcgc cagcttctga acgcaaagct gatcactcag cggaagttcg 3541 acaatctcac taaggctgag aggggcggac tgagcgaact ggacaaagca ggattcatta 3601 aacggcaact tgtggagact cggcagatta ctaaacatgt agcccaaatc cttgactcac 3661 gcatgaatac caagtacgac gaaaacgaca aacttatccg cgaggtgaag gtgattaccc 3721 tgaagtccaa gctggtcagc gatttcagaa aggactttca attctacaaa gtgcgggaga 3781 tcaataacta tcatcatgct catgacgcat atctgaatgc cgtggtggga accgccctga 3841 tcaagaagta cccaaagctg gaaagcgagt tcgtgtacgg agactacaag gtctacgacg 3901 tgcgcaagat gattgccaaa tctgagcagg agatcggaaa ggccaccgca aagtacttct 3961 tctacagcaa catcatgaat ttcttcaaga ccgaaatcac ccttgcaaac ggtgagatcc 4021 ggaagaggcc gctcatcgag actaatgggg agactggcga aatcgtgtgg gacaagggca 4081 gagatttcgc taccgtgcgc aaagtgcttt ctatgcctca agtgaacatc gtgaagaaaa 4141 ccgaggtgca aaccggaggc ttttctaagg aatcaatcct ccccaagcgc aactccgaca 4201 agctcattgc aaggaagaag gattgggacc ctaagaagta cggcggattc gattcaccaa 4261 ctgtggctta ttctgtcctg gtcgtggcta aggtggaaaa aggaaagtct aagaagctca 4321 agagcgtgaa ggaactgctg ggtatcacca ttatggagcg cagctccttc gagaagaacc 4381 caattgactt tctcgaagcc aaaggttaca aggaagtcaa gaaggacctt atcatcaagc 4441 tcccaaagta tagcctgttc gaactggaga atgggcggaa gcggatgctc gcctccgctg 4501 gcgaacttca gaagggtaat gagctggctc tcccctccaa gtacgtgaat ttcctctacc 4561 ttgcaagcca ttacgagaag ctgaagggga gccccgagga caacgagcaa aagcaactgt 4621 ttgtggagca gcataagcat tatctggacg agatcattga gcagatttcc gagttttcta 4681 aacgcgtcat tctcgctgat gccaacctcg ataaagtcct tagcgcatac aataagcaca 4741 gagacaaacc aattcgggag caggctgaga atatcatcca cctgttcacc ctcaccaatc 4801 ttggtgcccc tgccgcattc aagtacttcg acaccaccat cgaccggaaa cgctatacct 4861 ccaccaaaga agtgctggac gccaccctca tccaccagag catcaccgga ctttacgaaa 4921 ctcggattga cctctcacag ctcggagggg atgagggagc tcccaagaaa aagcgcaagg 4981 taggtagttc caagcttggc ggcagcggcg gcagcatgga acgtgtgaga atgattaatg 5041 tgcaaaggct gttagaagcc gcagagtttt tagaaagaag agaaagagaa tgcgaacacg 5101 ggtatgccag ttctttccct agcatgccct ctcccagagg ctaactcgag gcgaatttct 5161 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt 5221 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg 5281 tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca 5341 tgccgagcaa atgcctgcaa atcgctcccc atttctctag ataagactgc agagttcggt 5401 accagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag 5461 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 5521 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 5581 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 5641 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 5701 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 5761 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 5821 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 5881 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 5941 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 6001 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 6061 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 6121 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 6181 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 6241 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 6301 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 6361 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 6421 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 6481 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 6541 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 6601 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 6661 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 6721 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 6781 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 6841 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 6901 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 6961 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 7021 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 7081 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 7141 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 7201 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 7261 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 7321 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 7381 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 7441 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 7501 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 7561 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc cttttcatca 7621 cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa ttaaaaatag 7681 aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg taaaagactc 7741 tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc aggtattaat 7801 gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg tgattttaca 7861 ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt ctaataaata 7921 tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt ttttttcttc 7981 attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca aaacataaaa 8041 ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac gaggcgcgtg 8101 taagttacag gcaagcgatc cgtctaagaa accattatta tcatgacatt aacctataaa 8161 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc 8221 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga 8281 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg 8341 gcatcagagc agattgtact gagagtgcac catatcgact acgtcgttaa ggccgtttct 8401 gacagagtaa aattcttgag ggaactttca ccattatggg aaatggttca agaaggtatt 8461 gacttaaact ccatcaaatg gtcaggtcat tgagtgtttt ttatttgttg tatttttttt 8521 tttttagaga aaatcctcca atatataaat taggaatcat agtttcatga ttttctgtta 8581 cacctaactt tttgtgtggt gccctcctcc ttgtcaatat taatgttaaa gtgcaattct 8641 ttttccttat cacgttgagc cattagtatc aatttgctta cctgtattcc tttacatcct 8701 cctttttctc cttcttgata aatgtatgta gattgcgtat atagtttcgt ctaccctatg 8761 aacatattcc attttgtaat ttcgtgtcgt ttctattatg aatttcattt ataaagttta 8821 tgtacaaata tcataaaaaa agagaatctt tttaagcaag gattttctta acttcttcgg 8881 cgacagcatc accgacttcg gtggtactgt tggaaccacc taaatcacca gttctgatac 8941 ctgcatccaa aaccttttta actgcatctt caatggcctt accttcttca ggcaagttca 9001 atgacaattt caacatcatt gcagcagaca agatagtggc gatagggttg accttattct 9061 ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt 9121 ctggcaaaga ggccaaggac gcagatggca acaaacccaa ggaacctggg ataacggagg 9181 cttcatcgga gatgatatca ccaaacatgt tgctggtgat tataatacca tttaggtggg 9241 ttgggttctt aactaggatc atggcggcag aatcaatcaa ttgatgttga accttcaatg 9301 taggaaattc gttcttgatg gtttcctcca cagtttttct ccataatctt gaagaggcca 9361 aaacattagc tttatccaag gaccaaatag gcaatggtgg ctcatgttgt agggccatga 9421 aagcggccat tcttgtgatt ctttgcactt ctggaacggt gtattgttca ctatcccaag 9481 cgacaccatc accatcgtct tcctttctct taccaaagta aatacctccc actaattctc 9541 tgacaacaac gaagtcagta cctttagcaa attgtggctt gattggagat aagtctaaaa 9601 gagagtcgga tgcaaagtta catggtctta agttggcgta caattgaagt tctttacgga 9661 tttttagtaa accttgttca ggtctaacac taccggtacc ccatttagga ccacccacag 9721 cacctaacaa aacggcatca gccttcttgg aggcttccag cgcctcatct ggaagtggaa 9781 cacctgtagc atcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat 9841 tggaacgaac atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac 9901 caacgtggtc acctggcaaa acgacgatct tcttaggggc agacattaga atggtatatc 9961 cttgaaatat atatatatat attgctgaaa tgtaaaaggt aagaaaagtt agaaagtaag 10021 acgattgcta accacctatt ggaaaaaaca ataggtcctt aaataatatt gtcaacttca 10081 agtattgtga tgcaagcatt tagtcatgaa cgcttctcta ttctatatga aaagccggtt 10141 ccggcgctct cacctttcct ttttctccca atttttcagt tgaaaaaggt atatgcgtca 10201 ggcgacctct gaaattaaca aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg 10261 cccctgtgtg ttctcgttat gttgaggaaa aaaataatgg ttgctaagag attcgaactc 10321 ttgcatctta cgatacctga gtattcccac agttaactgc ggtcaagata tttcttgaat 10381 caggcgcctt agaccgctcg gccaaacaac caattacttg ttgagaaata gagtataatt 10441 atcctataaa tataacgttt ttgaacacac atgaacaagg aagtacagga caattgattt 10501 tgaagagaat gtggattttg atgtaattgt tgggattcca tttttaataa ggcaataata 10561 ttaggtatgt agatatacta gaagttctcc tcgacggtcg atatgcggtg tgaaataccg 10621 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa 10681 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 10741 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 10801 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 10861 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 10921 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 10981 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 11041 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 11101 agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 11161 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaag //