LOCUS       Exported                6078 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  AAV expression of EYFP from CaMKII(1.3) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV.CamKII(1.3).eYFP.WPRE.hGH
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6078)
  TITLE     Deisseroth AAV plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   75..204
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        223..1511
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             1535..2254
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1580..1601)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1841..1860)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2188..2209
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2279..2867
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2332..2352)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2750..2761)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2899..3375
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3098..3117)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3415..3555
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3415..3544
                     /label=AAV2 ITR
     rep_origin      3630..4085
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3717..3736)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3927..3948
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4102..4121)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4221..4243
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4281..4299)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4367..4471
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4472..5332
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4690..4709)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5503..13
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5992..6011
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
       61 ttttgctcac atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc
      121 gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg
      181 ccaactccat cactaggggt tcctgcggcc gcacgcgttt aacattatgg ccttaggtca
      241 cttcatctcc atggggttct tcttctgatt ttctagaaaa tgagatgggg gtgcagagag
      301 cttcctcagt gacctgccca gggtcacatc agaaatgtca gagctagaac ttgaactcag
      361 attactaatc ttaaattcca tgccttgggg gcatgcaagt acgatataca gaaggagtga
      421 actcattagg gcagatgacc aatgagttta ggaaagaaga gtccagggca gggtacatct
      481 acaccacccg cccagccctg ggtgagtcca gccacgttca cctcattata gttgcctctc
      541 tccagtccta ccttgacggg aagcacaagc agaaactggg acaggagccc caggagacca
      601 aatcttcatg gtccctctgg gaggatgggt ggggagagct gtggcagagg cctcaggagg
      661 ggccctgctg ctcagtggtg acagataggg gtgagaaagc agacagagtc attccgtcag
      721 cattctgggt ctgtttggta cttcttctca cgctaaggtg gcggtgtgat atgcacaatg
      781 gctaaaaagc agggagagct ggaaagaaac aaggacagag acagaggcca agtcaaccag
      841 accaattccc agaggaagca aagaaaccat tacagagact acaaggggga agggaaggag
      901 agatgaatta gcttcccctg taaaccttag aacccagctg ttgccagggc aacggggcaa
      961 tacctgtctc ttcagaggag atgaagttgc cagggtaact acatcctgtc tttctcaagg
     1021 accatcccag aatgtggcac ccactagccg ttaccatagc aactgcctct ttgccccact
     1081 taatcccatc ccgtctgtta aaagggccct atagttggag gtgggggagg taggaagagc
     1141 gatgatcact tgtggactaa gtttgttcgc atccccttct ccaaccccct cagtacatca
     1201 ccctggggga acagggtcca cttgctcctg ggcccacaca gtcctgcagt attgtgtata
     1261 taaggccagg gcaaagagga gcaggtttta aagtgaaagg caggcaggtg ttggggaggc
     1321 agttaccggg gcaacgggaa cagggcgttt cggaggtggt tgccatgggg acctggatgc
     1381 tgacgaaggc tcgcgaggct gtgagcagcc acagtgccct gctcagaagc cccaagctcg
     1441 tcagtcaagc cggttctccg tttgcactca ggagcacggg caggcgagtg gcccctagtt
     1501 ctgggggcag ctctagagcg gtaccggatc caccatggtg agcaagggcg aggagctgtt
     1561 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1621 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1681 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct
     1741 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1801 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1861 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1921 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1981 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     2041 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     2101 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag
     2161 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     2221 gatcactctc ggcatggacg agctgtacaa gtaagaattc gatatcaagc ttatcgataa
     2281 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     2341 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     2401 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     2461 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     2521 ttggggcatt gccaccacct gtcagctcct ttccggaact ttcgctttcc ccctccctat
     2581 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     2641 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     2701 ctatgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     2761 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     2821 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc
     2881 gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
     2941 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
     3001 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
     3061 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
     3121 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
     3181 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
     3241 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
     3301 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
     3361 cccttccctg tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac
     3421 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     3481 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     3541 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3601 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     3661 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
     3721 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3781 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3841 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3901 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     3961 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
     4021 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     4081 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     4141 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4201 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     4261 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4321 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4381 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4441 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4501 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4561 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4621 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4681 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4741 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4801 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4861 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4921 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4981 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5041 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5101 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5161 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5221 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5281 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5341 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5401 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5461 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5521 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5581 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5641 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5701 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5761 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5821 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5881 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5941 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6001 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6061 atgctcgtca ggggggcg
//
