LOCUS       Exported                5503 bp ds-DNA     circular SYN 05-DEC-2022
DEFINITION  AAV expression of Cre from GfaABC1D promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV.GfaABC1D.PI.Cre.SV40
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5503)
  TITLE     Baljit Khakh lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          793..925
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     908..927
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        970..988
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1011..2042
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     polyA_signal    2082..2203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2093..2112)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2147..2166
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2365..2494
                     /label=AAV2 ITR
     primer_bind     complement(2532..2548)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2532..2548)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2545..2567)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2556..2572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2580..2610)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2625..2646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2763..2780)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2934..3522)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3014..3033)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3693..4553)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4316..4335
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4554..4658)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4684..5139)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4821..4842)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5033..5052
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5266..5288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5280..5297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5281..5297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   5316..5445
                     /label=AAV2 ITR
ORIGIN
        1 ctaacatatc ctggtgtgga gtaggggacg ctgctctgac agaggctcgg gggcctgagc
       61 tggctctgtg agctggggag gaggcagaca gccaggcctt gtctgcaagc agacctggca
      121 gcattgggct ggccgccccc cagggcctcc tcttcatgcc cagtgaatga ctcaccttgg
      181 cacagacaca atgttcgggg tgggcacagt gcctgcttcc cgccgcaccc cagcccccct
      241 caaatgcctt ccgagaagcc cattgagcag ggggcttgca ttgcacccca gcctgacagc
      301 ctggcatctt gggataaaag cagcacagcc ccctaggggc tgcccttgct gtgtggcgcc
      361 accggcggtg gagaacaagg ctctattcag cctgtgccca ggaaagggga tcaggggatg
      421 cccaggcatg gacagtgggt ggcagggggg gagaggaggg ctgtctgctt cccagaagtc
      481 caaggacaca aatgggtgag gggagagctc tccccatagc tgggctgcgg cccaacccca
      541 ccccctcagg ctatgccagg gggtgttgcc aggggcaccc gggcatcgcc agtctagccc
      601 actccttcat aaagccctcg catcccagga gcgagcagag ccagagcagg ttggagagga
      661 gacgcatcac ctccgctgct cgcaagcttt attgcggtag tttatcacag ttaaattgct
      721 aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga
      781 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
      841 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
      901 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc
      961 tagagtactt aatacgactc actataggct agcctcgaga attcgccacc atgtccaatt
     1021 tactgaccgt acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc
     1081 gcaagaacct gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa
     1141 tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt
     1201 ttcccgcaga acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg
     1261 cagtaaaaac tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc
     1321 tgccacgacc aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa
     1381 acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc
     1441 aggttcgttc actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc
     1501 tggggattgc ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag
     1561 atatctcacg tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg
     1621 ttagcaccgc aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat
     1681 ggatttccgt ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa
     1741 aaaatggtgt tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga
     1801 tttttgaagc aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc
     1861 tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag
     1921 tttcaatacc ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact
     1981 atatccgtaa cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatt
     2041 agtctagagt cgacccgggc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     2101 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2161 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2221 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     2281 ctacaaatgt ggtaaaatcg ataaggatct tcctagagca tggctacgta gataagtagc
     2341 atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc
     2401 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     2461 cccgggcggc ctcagtgagc gagcgagcgc gcagcctaat taaggcctta attaaatctg
     2521 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     2581 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     2641 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     2701 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2761 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2821 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2881 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2941 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3001 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3061 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3121 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3181 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3241 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3301 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3361 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3421 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3481 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3541 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3601 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3661 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3721 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3781 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3841 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3901 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3961 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     4021 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4081 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4141 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4201 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4261 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4321 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4381 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4441 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4501 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4561 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4621 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4681 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     4741 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     4801 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     4861 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     4921 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     4981 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     5041 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     5101 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
     5161 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     5221 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     5281 gtaaaacgac ggccagtgaa ttaggttaat taaggctgcg cgctcgctcg ctcactgagg
     5341 ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc
     5401 gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag ttaatgatta
     5461 acccgccatg ctacttatct acgtagccat gctctaggaa gat
//
