LOCUS       Exported                5889 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  AAV expression of firefly luciferase from CMV promoter
             .
ACCESSION   .
VERSION     .
KEYWORDS    pAAV.CMV.ffLuciferase.SV40
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5889)
  TITLE     AAV plasmids
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        199..578
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        579..782
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     733..753
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     779..803
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      838..847
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             844..2496
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2527..2648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2538..2557)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2592..2611
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2810..2939
                     /label=AAV2 ITR
     primer_bind     complement(2958..2975)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2958..2974)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2967..2989)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3116..3571
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3203..3222)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3413..3434
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3597..3701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3702..4562
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3920..3939)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4733..5321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5222..5241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5475..5492
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5609..5630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5645..5675
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5683..5699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5688..5710
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5707..5723
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5707..5723
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5761..1
                     /label=AAV2 ITR
ORIGIN
        1 ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc taggaagatc
       61 ttcaatattg gccattagcc atattattca ttggttatat agcataaatc aatattggct
      121 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
      181 caatatgacc gccatgttgg cattgattat tgactagtta ttaatagtaa tcaattacgg
      241 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      301 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      361 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      421 cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg
      481 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt
      541 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      601 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      661 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc
      721 ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      781 ctcgtttagt gaaccgtcag atcactagaa gcttggcaat ccggtactgt tggtaaagcc
      841 accatggaag atgccaaaaa cattaagaag ggcccagcgc cattctaccc actcgaagac
      901 gggaccgccg gcgagcagct gcacaaagcc atgaagcgct acgccctggt gcccggcacc
      961 atcgccttta ccgacgcaca tatcgaggtg gacattacct acgccgagta cttcgagatg
     1021 agcgttcggc tggcagaagc tatgaagcgc tatgggctga atacaaacca tcggatcgtg
     1081 gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt tgggtgccct gttcatcggt
     1141 gtggctgtgg ccccagctaa cgacatctac aacgagcgcg agctgctgaa cagcatgggc
     1201 atcagccagc ccaccgtcgt attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg
     1261 caaaagaagc taccgatcat acaaaagatc atcatcatgg atagcaagac cgactaccag
     1321 ggcttccaaa gcatgtacac cttcgtgact tcccatttgc cacccggctt caacgagtac
     1381 gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg ccctgatcat gaacagtagt
     1441 ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc
     1501 agtcatgccc gcgaccccat cttcggcaac cagatcatcc ccgacaccgc tatcctcagc
     1561 gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc tgggctactt gatctgcggc
     1621 tttcgggtcg tgctcatgta ccgcttcgag gaggagctat tcttgcgcag cttgcaagac
     1681 tataagattc aatctgccct gctggtgccc acactattta gcttcttcgc taagagcact
     1741 ctcatcgaca agtacgacct aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc
     1801 agcaaggagg taggtgaggc cgtggccaaa cgcttccacc taccaggcat ccgccagggc
     1861 tacggcctga cagaaacaac cagcgccatt ctgatcaccc ccgaagggga cgacaagcct
     1921 ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg tggtggactt ggacaccggt
     1981 aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc
     2041 ggctacgtta acaaccccga ggctacaaac gctctcatcg acaaggacgg ctggctgcac
     2101 agcggcgaca tcgcctactg ggacgaggac gagcacttct tcatcgtgga ccggctgaag
     2161 agcctgatca aatacaaggg ctaccaggta gccccagccg aactggagag catcctgctg
     2221 caacacccca acatcttcga cgccggggtc gccggcctgc ccgacgacga tgccggcgag
     2281 ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg
     2341 gactatgtgg ccagccaggt tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg
     2401 gacgaggtgc ctaaaggact gaccggcaag ttggacgccc gcaagatccg cgagattctc
     2461 attaaggcca agaagggcgg caagatcgcc gtgtaataat tctagggccg cttcgagcag
     2521 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     2581 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     2641 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     2701 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatcttccta
     2761 gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta
     2821 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     2881 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     2941 cttaattaac ctaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3001 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3061 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     3121 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3181 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3241 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3301 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3361 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3421 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3481 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3541 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     3601 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3661 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3721 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3781 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3841 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3901 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     3961 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4021 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4081 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4141 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4201 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4261 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4321 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4381 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4441 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4501 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4561 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4621 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4681 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4741 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4801 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4861 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4921 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4981 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5041 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5101 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5161 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5221 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5281 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5341 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5401 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5461 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5521 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     5581 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     5641 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     5701 ttcacacagg aaacagctat gaccatgatt acgccagatt taattaaggc cttaattagg
     5761 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
     5821 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
     5881 aggggttcc
//
