LOCUS       Exported                4921 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  AAV expression of Cre from CamKII promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pENN.AAV.CamKII 0.4.Cre.SV40
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4921)
  TITLE     AAV plasmids
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             436..1467
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     polyA_signal    1520..1641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1531..1550)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1585..1604
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   1803..1932
                     /label=AAV2 ITR
     primer_bind     complement(1951..1968)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1951..1967)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1960..1982)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2109..2564
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2196..2215)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2406..2427
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2590..2694
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2695..3555
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2913..2932)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3726..4314
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4215..4234
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4468..4485
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4602..4623
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4638..4668
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4676..4692
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4681..4703
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4700..4716
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4700..4716
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tagcatgcct aacttgtgga ctaagtttgt tcacatcccc ttctccaacc ccctcagtac
       61 atcaccctgg gggaacaggg tccacttgct cctgggccca cacagtcctg cagtattgtg
      121 tatataaggc cagggcaaag aggagcaggt tttaaagtga aaggcaggca ggtgttgggg
      181 aggcagttac cggggcaacg ggaacagggc gtttcggagg tggttgccat ggggacctgg
      241 atgctgacga aggctcgcga ggctgtgagc agccacagtg ccctgctcag aagccccaag
      301 ctcgtcagtc aagccggttc tccgtttgca ctcaggagca cgggcaggcg agtggcccct
      361 agttctgggg gcagctctag agccgatccc ctcgagaatt gtacacttta cttaaaacca
      421 ttatctgagt gtgaaatgtc caatttactg accgtacacc aaaatttgcc tgcattaccg
      481 gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag
      541 gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg
      601 tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt
      661 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta
      721 aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg
      781 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta
      841 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc
      901 caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc
      961 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc
     1021 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg
     1081 ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat
     1141 aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag
     1201 ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct
     1261 aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc
     1321 gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg
     1381 accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg
     1441 gtgcgcctgc tggaagatgg cgattagcca ttaacgcggc gtggtacctc tagagtcggg
     1501 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1561 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1621 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     1681 ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     1741 aaggatcttc ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta
     1801 caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     1861 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     1921 gcgagcgcgc agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg
     1981 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     2041 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     2101 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2161 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     2221 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2281 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2341 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2401 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     2461 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     2521 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     2581 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2641 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2701 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2761 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2821 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2881 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2941 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3001 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3061 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     3121 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3181 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3241 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3301 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3361 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3421 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3481 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3541 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3601 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3661 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3721 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3781 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3841 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3901 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3961 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4021 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4081 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4141 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4201 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4261 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4321 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4381 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4441 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4501 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     4561 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     4621 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     4681 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccag atttaattaa
     4741 ggccttaatt aggctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg
     4801 gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc
     4861 ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc taggaagatc
     4921 c
//
