LOCUS       Exported               10407 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-msCFPx6..
ACCESSION   .
VERSION     .
KEYWORDS    YIplac211-SEC7-msCFPx6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Day KJ, Casler JC, Glick BS
  TITLE     Budding Yeast Has a Minimal Endomembrane System.
  JOURNAL   Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi: 
            10.1016/j.devcel.2017.12.014. Epub 2018 Jan 8.
  PUBMED    29316441
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10407
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7065..7081)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7065..7081)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7078..7100)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7089..7105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7113..7143)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7158..7179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7296..7313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7467..8055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7547..7566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8226..9086)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8849..8868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9087..9191)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(9321..10121)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQLN"
     promoter        complement(10122..10337)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     10380..10398
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctattgttgt caaatgtgtt
      421 ttacagcttt tgatgataga gctgctcaac gaattattcg agaacgaaga ttttgcccac
      481 tgtatccctt acaaggaagc cattagaatt acaagattgt tggagaaatc gtacgaattt
      541 tctcgtgact ttaatgaaga ttatgggtta agaacaagac tagtagaggc tcgtgtagtt
      601 gataaaatac caaacctgtt gaaacaagaa acgagtgctg ctgcagttct ccttgacatt
      661 atgtttcagt tgtatctgaa cgatgatgag aagaaggctg atttaataac tcgtctgatc
      721 accatttgca tccaggtcgt agagggttat gtttctttgg atgatagaac catggaacgt
      781 agtatcaatg cctggcgttc agtgatagtt gaaatattac aaggttacta cgaatttgat
      841 gatgaagact ttagactata ctgtcctgct atgtatgctc tggtgattca aatattagat
      901 aagagcgttc caacggagtt gagacatgct ataaaacaat ttctaagcag agttggtgaa
      961 ttataccttt ctacggatcc aggaagttcg gggtccagcg gttcttcagg cgctagcatg
     1021 gatagcactg agagcctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1081 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     1141 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1201 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     1261 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat caccttcaag
     1321 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1381 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1441 gagtacaact tcatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     1501 aaggccaact tcaagatccg ccacaacgtg gaggacggcg gcgtgcagct cgccgaccac
     1561 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1621 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1681 gagttcgtga ccgccgccgg gatcactcac ggcagctccg gctccagcgg gtctagcatg
     1741 gatagcactg agagcctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1801 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     1861 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1921 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     1981 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat caccttcaag
     2041 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2101 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2161 gagtacaact tcatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     2221 aaggccaact tcaagatccg ccacaacgtg gaggacggcg gcgtgcagct cgccgaccac
     2281 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2341 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2401 gagttcgtga ccgccgccgg gatcactcac ggcagctccg gctccagcgg gtctagcatg
     2461 gatagcactg agagcctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2521 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     2581 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2641 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     2701 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat caccttcaag
     2761 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2821 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2881 gagtacaact tcatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     2941 aaggccaact tcaagatccg ccacaacgtg gaggacggcg gcgtgcagct cgccgaccac
     3001 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3061 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3121 gagttcgtga ccgccgccgg gatcactcac ggcagctccg gctccagcgg gtctagcatg
     3181 gatagcactg agagcctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3241 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     3301 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3361 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     3421 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat caccttcaag
     3481 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3541 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3601 gagtacaact tcatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     3661 aaggccaact tcaagatccg ccacaacgtg gaggacggcg gcgtgcagct cgccgaccac
     3721 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3781 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3841 gagttcgtga ccgccgccgg gatcactcac ggcagctccg gctccagcgg gtctagcatg
     3901 gatagcactg agagcctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3961 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     4021 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4081 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     4141 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat caccttcaag
     4201 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4261 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4321 gagtacaact tcatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     4381 aaggccaact tcaagatccg ccacaacgtg gaggacggcg gcgtgcagct cgccgaccac
     4441 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4501 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     4561 gagttcgtga ccgccgccgg gatcactcac ggcagctccg gctccagcgg gtctagcatg
     4621 gatagcactg agagcctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4681 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     4741 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4801 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     4861 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat caccttcaag
     4921 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4981 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     5041 gagtacaact tcatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     5101 aaggccaact tcaagatccg ccacaacgtg gaggacggcg gcgtgcagct cgccgaccac
     5161 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     5221 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5281 gagttcgtga ccgccgccgg gatcactcac ggcagctccg gctccagcgg gtaaagcggc
     5341 cgggctttgt tgaatggtcc agcagattaa aatatgctta gttgtagaac acagaaaaaa
     5401 tgagaaggtt gatatacttt tactcatatt taccctgatt taatggcggt gatccttttt
     5461 tgattcgttt aattcataaa caaggtacat catatagcaa taacaaatat gtaaactatg
     5521 tagaaagact tatcaagttt agccatcttg gtcctcattc atgggcattt tatactaact
     5581 tcatttttgc cgaaataaga aaggatgtaa tcttaaccta aaatatgtaa gcaattaagg
     5641 gtgcacgtag ttacttgcga acaaatagga aaagaaagaa acaagcacgg atactgaatg
     5701 actgagtgta ttactacacg atttatctca acgaatttaa gtgtggagga cgatcggctg
     5761 cacgtaggag tcaacgtttt tttactgtgt tcatcacaaa accattaagc tgagtcatcc
     5821 atattgcaaa caataaggta gcaccgcttc catcaaaaca ctagaacata ccgatgtttt
     5881 cacggattta ttggaaacaa atagcagatg agggcgtttt tacttgtaat gaaggcgaat
     5941 gagaaggcaa ggcttacagg cttgttgttt atgccaagca tcaatactcg ctatggacga
     6001 gcgccatcag cgataacaac aaagtttgca agtgcttgct tggatataaa aggcttgacg
     6061 cttaagataa atagggccat acttgcttgc ttttactatt tttgttcctc ataagcctga
     6121 ttgactacgc tattataaac ccctacatta tcctataatc aacttcactt gtgtaacgtt
     6181 gaatgagttc aactgagtaa tactcggtcg gaaatatacg ctccatctgg caggaaacat
     6241 aggctgcatt tatatcttgc acaattatgc ccttcgtagg tgtatgtttt catgttttcc
     6301 attatgtcaa tgtagatgta tctttggaca ggcaactttt ggatcaataa agcacaattt
     6361 ttgagcaatt tgagatatcc agtatttgta cgggagtccc aagattgttt agcctaaaag
     6421 gttgaggact tggattattc ttccatcaag cgcctccacc cctcgcatcc tatttctttt
     6481 gaagcatagg ggacgagact gtctgtaatt tatggcgaag tatgacaaga aagattcaaa
     6541 ctttgtttta cgtatgcccc aataaattct atggtagcac tactagagac gtattttggt
     6601 tttgtaataa ttttcaaact gctcgctatg atttgttaca agaaacaaac aaaagacacg
     6661 accatagggt tcatgttggt ggttgcgaag ccacatcttt aaaacattac tgcaaattca
     6721 gcaggagtga gtagcaaaat cgacaaaaac tagagtacgc aacattcccg gtcgtaaatt
     6781 tcttatcgtg gcgccagaac attcccgtcc aatgatgaga atgtctctaa aacaaatgca
     6841 gtcacagaga ttttctgcga cttctcgagt gagaatgttg cttattggtg ctagtggctt
     6901 gagatcttcg tcaagcaaat tggaatgccc ccagattctc aaacaagcac ggcagaaact
     6961 gaaatacggc caaaattatg aacccttttg tgggatcttt gtagaaatag gacctcagga
     7021 tagtatgatg aagaatgttg gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     7081 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7141 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7201 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7261 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7321 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7381 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7441 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7501 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7561 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7621 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7681 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7741 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7801 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7861 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7921 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7981 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8041 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8101 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8161 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8221 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8281 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8341 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8401 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8461 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8521 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8581 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8641 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8701 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8761 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8821 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8881 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8941 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9001 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9061 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9121 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9181 ggggttccgc gcacatttcc ccgaaaagtg ccacctagct ttttctttcc aatttttttt
     9241 ttttcgtcat tataaaaatc attacgaccg agattcccgg gtaataactg atataattaa
     9301 attgaagctc taatttgtga gtttagctgg ccgcatcttc tcaaatatgc ttcccagcct
     9361 gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa tagtcctctt
     9421 ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct atactgttga
     9481 cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa
     9541 ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa atctttgtcg
     9601 ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga gcccttgcat
     9661 gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc tgccgcctgc
     9721 ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat gtctgcccat
     9781 tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc aatgtcagca
     9841 aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt tagcggctta
     9901 actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag taaacaaatt
     9961 ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac atccaatgaa
    10021 gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc aacaggacta
    10081 ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct tcgtttcctg
    10141 caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca
    10201 ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt
    10261 tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa agaataaaaa
    10321 aaaaatgatg aattgaaaag ctctaagaaa ccattattat catgacatta acctataaaa
    10381 ataggcgtat cacgaggccc tttcgtc
//
