LOCUS Exported 5407 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Genetically encoded hydrogen peroxide indicator with red fluorescence.. ACCESSION . VERSION . KEYWORDS pC1-HyPer-Red SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5407) AUTHORS Ermakova YG, Bilan DS, Matlashov ME, Mishina NM, Markvicheva KN, Subach OM, Subach FV, Bogeski I, Hoth M, Enikolopov G, Belousov VV TITLE Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide. JOURNAL Nat Commun. 2014 Oct 21;5:5222. doi: 10.1038/ncomms6222. PUBMED 25330925 REFERENCE 2 (bases 1 to 5407) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5407 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 1..589 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 490..509 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 764..1067 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1068..1271 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 1222..1242 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 1301..1310 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1307..2734 /codon_start=1 /product="red fluorescent genetically encoded indicator for H2O2 ()" /label=HyPerRed /translation="MEMASQQGETMSGPLHIGLIPTVGPYLLPHIIPMLHQTFPKLEMY LHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVP MADLAGEKLLMLEDGHCLRDQAMPRTVSERMYPEDGALKSEIKKGLRLKDGGHYAAEVK TTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQCERAEGRHSTGGMDELYKGGTGGS LVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPL PFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSL QDGVFIYKVKLRGTNFPPDGPVMQKKTMGWEAAGVGFCFEAGADEDTHFRATSLETLRN MVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGSPLRSRYEQLAEA IRARMDGHFDKVLKQAV" primer_bind 2386..2405 /label=mCherry-F /note="mCherry, forward primer" polyA_signal 2898..3019 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2935..2954) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2989..3008 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3026..3481) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3163..3184) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3375..3394 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3508..3612 /gene="bla" /label=AmpR promoter promoter 3614..3971 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3637..3657) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3822..3957 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3884..3903 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4006..4800 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4060..4079) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4670..4689 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4988..5007) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5032..5079 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" ORIGIN 1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat 721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 1261 taagcagagc tggtttagtg aaccgtcaga tccgctagcc gccaccatgg agatggcgag 1321 ccagcagggc gagacgatgt ccggaccgct gcacattggt ttgattccca cagttggacc 1381 gtacctgcta ccgcatatta tccctatgct gcaccagacc tttccaaagc tggaaatgta 1441 tctgcatgaa gcacagaccc accagttact ggcgcaactg gacagcggca aactcgattg 1501 cgtgatcctc gcactggtga aagagagcga agcattcatt gaagtgccgt tgtttgatga 1561 gccaatgttg ctggctatct atgaagatca cccgtgggcg aaccgcgaat gcgtaccgat 1621 ggccgatctg gcaggggaaa aactgctgat gctggaagat ggtcactgtt tgcgcgatca 1681 ggcaatgccc cgcacggttt ccgagcggat gtaccccgag gacggcgccc tgaagagcga 1741 gatcaagaag gggctgaggc tgaaggacgg cggccactac gccgccgagg tcaagaccac 1801 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga 1861 catcgtgtcc cacaacgagg actacaccat cgtggaacag tgcgaacgcg ccgagggccg 1921 ccactccacc ggcggcatgg acgagctgta caagggaggt acaggcggga gtctggtgag 1981 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat 2041 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta 2101 cgaggccttt cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg 2161 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacattaagc acccagccga 2221 catccccgac tacttcaagc tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa 2281 cttcgaggac ggcggcatta ttcacgttaa ccaggactcc tccctgcagg acggcgtatt 2341 catctacaag gtgaagctgc gcggcaccaa cttccccccc gacggccccg taatgcagaa 2401 gaagaccatg ggctgggagg ctgccggggt gggtttctgt tttgaagccg gggcggatga 2461 agatacacac ttccgcgcga ccagcctgga aactctgcgc aacatggtgg cggcaggtag 2521 cgggatcact ttactgccag cgctggctgt gccgccggag cgcaaacgcg atggggttgt 2581 ttatctgccg tgcattaagc cggaaccacg ccgcactatt ggtctggttt atcgtcctgg 2641 ctcaccgctg cgcagccgct atgagcagct ggcagaggcc atccgcgcaa gaatggatgg 2701 ccatttcgat aaagttttaa aacaggcggt ttaatgagaa ttctgcagtc gacggtaccg 2761 cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag 2821 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 2881 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 2941 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 3001 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg 3061 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct 3121 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt 3181 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat 3241 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca 3301 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac 3361 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta 3421 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg 3481 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 3541 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 3601 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg 3661 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 3721 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 3781 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 3841 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 3901 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 3961 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 4021 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 4081 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 4141 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg 4201 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 4261 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 4321 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 4381 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 4441 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 4501 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc 4561 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 4621 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 4681 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 4741 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 4801 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 4861 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 4921 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga 4981 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa 5041 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc 5101 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt 5161 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg 5221 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa 5281 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 5341 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 5401 gatcttc //