LOCUS Exported 11249 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Doxycyline regulated expression of Tbx5 in mammalian cells. ACCESSION . VERSION . KEYWORDS pSAM2_mCherry_Tbx5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11249) AUTHORS Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs KM, Lyons GE, Kamp TJ TITLE Lineage Reprogramming of Fibroblasts into Proliferative Induced Cardiac Progenitor Cells by Defined Factors. JOURNAL Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 10.1016/j.stem.2015.12.001. Epub 2016 Feb 11. PUBMED 26877223 REFERENCE 2 (bases 1 to 11249) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11249 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 7..25 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 44..62 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 81..99 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 118..136 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 155..173 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 192..210 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" misc_feature 279..396 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" CDS complement(694..738) /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 923..1156 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 1653..1778 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" LTR 1825..2005 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" promoter complement(2023..2221) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(2048..2068) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 2223..2602 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" primer_bind 2774..2793 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 2868..2972 /gene="bla" /label=AmpR promoter CDS 2973..3833 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3191..3210) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4004..4592 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4493..4512 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4746..4763 /label=L4440 /note="L4440 vector, forward primer" protein_bind 4880..4901 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 4916..4946 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4954..4970 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 4959..4981 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 4978..4994 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 4978..4994 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" polyA_signal 5043..5164 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5054..5073) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 5108..5127 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(5294..5668) /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" promoter complement(5687..5734) /label=EM7 promoter /note="synthetic bacterial promoter " promoter complement(5782..6111) /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5796..5931 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(5850..5869) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 6096..6116 /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin complement(6125..6553) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6235..6256) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6447..6466 /label=F1ori-R /note="F1 origin, reverse primer" polyA_signal 6599..6823 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind 6812..6829 /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" LTR 6855..7035 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind 7544..7560 /label=KS primer /note="common sequencing primer, one of multiple similar variants" misc_feature 7563..8151 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS 7669..7680 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind 8078..8098 /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8184..8894) /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYGKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(8462..8481) /label=mCherry-F /note="mCherry, forward primer" primer_bind 8731..8749 /label=mCherry-R /note="mCherry, reverse primer" misc_feature 8899..9360 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind 9287..9304 /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" protein_bind 9430..9454 /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(11039..11063) /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(11136..11160) /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 11182..11200 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 11219..11237 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" ORIGIN 1 gtaccttctc tatcactgat agggagctcg agacgtgtcg acttctctat cactgatagg 61 gagacgttct agacgtacct tctctatcac tgatagggag ctcgagacgt gtcgacttct 121 ctatcactga tagggagacg ttctagacgt accttctcta tcactgatag ggagctcgag 181 acgtgtcgac ttctctatca ctgataggga gacgtcaggt ggcacttttc ggggacgcgt 241 aaccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt 301 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct 361 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt 421 ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat cgaatggatc tgtctctgtc 481 tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag 541 gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac 601 agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata attttatata 661 ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa 721 taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa 781 gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg 841 ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt 901 tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag 961 gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc 1021 ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc 1081 ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc 1141 aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc 1201 cttggtgggt gctactccta atggttcaat ttttactact ttatatttat ataattcact 1261 tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc 1321 tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg 1381 tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat 1441 ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag 1501 ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc 1561 catactatat gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc 1621 catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc 1681 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc 1741 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct 1801 ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga 1861 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag 1921 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc 1981 agatctggtc taaccagaga gacccagtac aggcaaaacg cgctgcttat atagacctcc 2041 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga 2101 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt 2161 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc 2221 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag 2281 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc 2341 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc 2401 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt 2461 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat 2521 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa 2581 taactagtca ataatcaatg tcaacgcgta tatctggccc gtacatcgcg aagcagcgca 2641 aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta 2701 gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac 2761 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatag gggatcggga 2821 gatctcccga tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 2881 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 2941 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 3001 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 3061 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 3121 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 3181 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 3241 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 3301 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 3361 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 3421 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 3481 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 3541 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 3601 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 3661 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 3721 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 3781 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 3841 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 3901 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 3961 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 4021 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 4081 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 4141 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 4201 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 4261 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 4321 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 4381 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 4441 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 4501 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 4561 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 4621 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 4681 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 4741 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 4801 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 4861 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 4921 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 4981 gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgacggt atacagacat 5041 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt 5101 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca 5161 agttggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg 5221 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat 5281 ctcgtagcac gtgtcagtcc tgctcctcgg ccacgaagtg cacgcagttg ccggccgggt 5341 cgcgcagggc gaactcccgc ccccacggct gctcgccgat ctcggtcatg gccggcccgg 5401 aggcgtcccg gaagttcgtg gacacgacct ccgaccactc ggcgtacagc tcgtccaggc 5461 cgcgcaccca cacccaggcc agggtgttgt ccggcaccac ctggtcctgg accgcgctga 5521 tgaacagggt cacgtcgtcc cggaccacac cggcgaagtc gtcctccacg aagtcccggg 5581 agaacccgag ccggtcggtc cagaactcga ccgctccggc gacgtcgcgc gcggtgagca 5641 ccggaacggc actggtcaac ttggccatgg tttagttcct caccttgtcg tattatacta 5701 tgccgatata ctatgccgat gattaattgt caacacgtgc tgatcagatc cgaaaatgga 5761 tatacaagct cccgggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta 5821 cttctggaat agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag 5881 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt 5941 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc 6001 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat 6061 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag 6121 aattaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 6181 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 6241 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 6301 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 6361 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 6421 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 6481 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 6541 ctacagggcg cgtggggata ccccctagag ccccagctgg ttctttccgc ctcagaagcc 6601 atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg 6661 tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt 6721 tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg 6781 ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcgg 6841 gtttaaacgg gccctgctag agattttcca cactgactaa aagggtctga gggatctcta 6901 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta 6961 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc 7021 taaccagaga gacccagtac agtccggatg cagctctcgg gccatgtgat gaaatgctag 7081 gcggctgtca aacctccact ctaatacttc tctctccggg tcatccatcc catgcaggct 7141 cacagggtgt aacaagcggg tgttctctcc ttcattggct tcttctacct tctcttgctc 7201 aactggtact agcttgtagc accatccaaa ggtcagtgga tatctgatcc ctggccctgg 7261 tgtgtagttc tgccaatcag ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat 7321 atcttgtctt cgttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg 7381 gctaagatct acagctgcct tgtaagtcat tggtcttaaa ggtacctgag gtgtgactgg 7441 aaaacccacc tcctcctcct cttgtgcttc tagccaggca caatcagcat tggtagctgc 7501 tgtattgcta cttgtgattg ctccatgttt ttctaggtct cgacgaggtc gacggtatcg 7561 atgcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg 7621 aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga 7681 agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca 7741 aggaaaggac gatgatttcc ccgacaacac cacggaattg tcagtgccca acagccgagc 7801 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg 7861 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt 7921 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc 7981 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc 8041 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata 8101 gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tatcgataag 8161 cttgatatcg aattatggtc gacttacttg tacagctcgt ccatgccgcc ggtggagtgg 8221 cggccctcgg cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg 8281 tccaacttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag 8341 gtggtcttga cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc 8401 tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg 8461 gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg 8521 tagatgaact cgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc 8581 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg 8641 gggatgtcgg cggggtgctt cccgtaggcc ttggagccgt acatgaactg aggggacagg 8701 atgtcccagg cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg 8761 ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag 8821 ccctccatgt gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg 8881 cccttgctca ccatggtatt atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt 8941 cggggggcct agacgttttt taacctcgac taaacacatg taaagcatgt gcaccgaggc 9001 cccagatcag atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat 9061 acgcttgagg tgagccattt gactctttcc acaactatcc aactcacaac gtggcactgg 9121 ggttgtgccg cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg 9181 tcgccaggtg gggggttccg ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa 9241 gaagcttcca gaggaactgc ttccttcacg acattcaaca gaccttgcat tcctttggcg 9301 agaggggaaa gacccctagg aatgctcgtc aagaagacag ggccaggttt ccgggccctc 9361 acattgccaa aagacggcaa tatggtggaa aataacgtcg actcatgtct gctcgaagcg 9421 gccgcatcaa ccactttgta caagaaagct gggtcgaatt cgccctttta gctattctca 9481 ctccactctg gcaccatgcc gacgccgtgt accgagtgat actgatgggg ggacagggtc 9541 ctgggcacgc cgtgagtgta gagaaactct gggggctgga ggccgcccgg agactgaagg 9601 ccagtctgag gcccgcactg cctgaccaca ggctgatggg ccactgaggt ctggtgctga 9661 aacatccctt cgccgagctg gggagaacca tggttggcca tgccagccaa ccgagggacc 9721 aggggccccg aggtgaaatg agcggagaag tgctggtagg gaagacggtc catgggctgc 9781 acggtggtga cggtacagct gctataggag ggcatgctgg gccatgtgtt acagctgatg 9841 tcctccaggc taggcacggg ctcgctgggg ggagcggagc tggcatacat gcaggcctgc 9901 cgctgggccg actctgtcct gtaagaggta ctcaggccct gctgctgggg gtagcccgag 9961 cgatagaagg tgtcttcctc gctgggggat gtctccatgt acggcttctt ataggggtgc 10021 tccgtgctgg aacattcctc atctttcctc ttggtacagt ggtaaatttg gctgtgctcc 10081 tgggccagtg ggtatgggtt aggtgggggc agaaggtcct gggaggggcc agagacacca 10141 ttctcacact ggtactggga ccctaaattg gatgaggtgg agagagctcg ggtctcgctg 10201 ctgaaggggc tgtggttgga ggtgactttg tgcctcattg tgctcctggg aaccacagga 10261 tactctttac tttgcatccg agacatcctg tgtaactcca ggtcatcact gccccgaaag 10321 cctttggcga aggggttgtt ctcaattttc agctgtgtga tcttgtgatt ctggtacgaa 10381 gtcacagcga taaaagctgt ctccgggaag acgtgggtgc aaaacgcagt gttctttgaa 10441 ccgaacccat tattttcgtc tgctttcacg atgtgtaatc ggggctggta tttgtgcatg 10501 gagttcagga taatgtgtcc aaacgggtcc aggtggttgt tggtgagttt gagcttctgg 10561 aaggagacaa gttgtcgcat ccagtgggct ccggttgctg gggagtccgg gtgcacgtaa 10621 aggcgccccg gcatggcagg ctctgctttg ccagttacgg accatttgtt atcagcaaat 10681 ttatatctgt ggtcgtctgc gggaacaata tccatgagaa gaatatactt cgttttggga 10741 ttaaggccag tcaccttcac tttgtaacta ggaaacattc tcctccctgc cttggtgatg 10801 atcatctctg tgcccacttc gtggaacttc agccacagtt cacgttcatg aagaaacacc 10861 ttgattcctt ccatgccctg ctgggtgaag gcagcctgcg gggatgatgg agacttgctg 10921 ggagccccca gagcactctc aggtttcgaa tcgcaagacc tgtctttgga atcaggctcc 10981 agaggcgtgc gcgccaggcc aaagccctca tctgtatcgg ccataagggc gaattcggag 11041 cctgcttttt tgtacaaact tgttgatgct agcaggctgg atcggtcccg gtgtcttcta 11101 tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct 11161 ctgcttatat agggggtacc ttctctatca ctgataggga gctcgagacg tgtcgacttc 11221 tctatcactg atagggagac gttctagac //