LOCUS       Exported                 582 bp ds-DNA     linear   SYN 30-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAG305GAL-ccdB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 582)
  TITLE     S cerevisiae Advanced Gateway Destination Vectors
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(119..243)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(276..292)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(277..293)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      295..542
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(311..329)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 cggggaagag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
       61 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca
      121 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
      181 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
      241 ggtgatgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct cgagtcatgt
      301 aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg
      361 aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat
      421 taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat
      481 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt
      541 gcggccgtcg agggggggcc cggtaccacc ttttgttccc tt
//
