LOCUS Exported 4732 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCMV/SB11 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4732) AUTHORS Ivics Z, Hackett PB, Plasterk RH, Izsvak Z TITLE Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. JOURNAL Cell. 1997 Nov 14. 91(4):501-10. PUBMED 9390559 REFERENCE 2 (bases 1 to 4732) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4732 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 76..98 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 90..107 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 91..107 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 134..437 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 438..641 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 592..612 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 638..662 /label=LNCX /note="Human CMV promoter, forward primer" intron 779..875 /label=SV40 intron /note="modified SV40 intron with splice donor and acceptor sites" polyA_signal 1978..2112 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2002..2021) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2056..2075 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(2223..2239) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2223..2239) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2236..2258) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 2247..2263 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2271..2301) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2316..2337 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2454..2471) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2625..3213) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2705..2724) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3384..4244) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4007..4026 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4245..4349) /gene="bla" /label=AmpR promoter primer_bind 4417..4435 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(4473..4495) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 4595..4614 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 61 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgagcttg 121 catgcctgca ggtcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 181 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 241 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 301 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 361 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 421 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 481 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 541 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 601 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga 661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca 721 gcctccggac tctagaggat ccggtactcg aggaactgaa aaaccagaaa gttaactggt 781 aagtttagtc tttttgtctt ttatttcagg tcccggatcc ggtggtggtg caaatcaaag 841 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc 901 tctaaaagct gcggaattgt acccgcggcc gatccgacat catgggaaaa tcaaaagaaa 961 tcagccaaga cctcagaaaa aaaattgtag acctccacaa gtctggttca tccttgggag 1021 caatttccaa acgcctgaaa gtaccacgtt catctgtaca aacaatagta cgcaagtata 1081 aacaccatgg gaccacgcag ccgtcatacc gctcaggaag gagacgcgtt ctgtctccta 1141 gagatgaacg tactttggtg cgaaaagtgc aaatcaatcc cagaacaaca gcaaaggacc 1201 ttgtgaagat gctggaggaa acaggtacaa aagtatctat atccacagta aaacgagtcc 1261 tatatcgaca taacctgaaa ggccgctcag caaggaagaa gccactgctc caaaaccgac 1321 ataagaaagc cagactacgg tttgcaagag cacatgggga caaagatcgt actttttgga 1381 gaaatgtcct ctggtctgat gaaacaaaaa tagaactgtt tggccataat gaccatcgtt 1441 atgtttggag gaagaagggg gaggcttgca agccgaagaa caccatccca accgtgaagc 1501 acgggggtgg cagcatcatg ttgtgggggt gctttgctgc aggagggact ggtgcacttc 1561 acaaaataga tggcatcatg aggaaggaaa attatgtgga tatattgaag caacatctca 1621 agacatcagt caggaagtta aagcttggtc gcaaatgggt cttccaacaa gacaatgacc 1681 ccaagcatac ttccaaacac gtgagaaaat ggcttaagga caacaaagtc aaggtattgg 1741 agtggccatc acaaagccct gacctcaatc ctatagaaaa tttgtgggca gaactgaaaa 1801 agcgtgtgcg agcaaggagg cctacaaacc tgactcagtt acaccagctc tgtcaggagg 1861 aatgggccaa aattcaccca acttattgtg ggaagcttgt ggaaggctac ccgaaacgtt 1921 tgacccaagt taaacaattt aaaggcaatg ctaccaaata ctagaattgg ccgcggggat 1981 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa 2041 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc 2101 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg 2161 tgggaggttt tttcggatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca 2221 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 2281 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 2341 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 2401 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 2461 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 2521 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 2581 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 2641 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 2701 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 2761 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 2821 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 2881 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 2941 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 3001 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 3061 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 3121 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 3181 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 3241 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 3301 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 3361 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 3421 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 3481 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 3541 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 3601 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 3661 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 3721 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 3781 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 3841 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 3901 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 3961 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 4021 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 4081 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 4141 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 4201 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 4261 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 4321 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 4381 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 4441 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 4501 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 4561 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga 4621 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 4681 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg gg //