LOCUS       Exported                 609 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAG423GPD-ccdB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 609)
  TITLE     S cerevisiae Advanced Gateway Destination Vectors
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(40..164)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(172..188)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(172..188)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(277..300)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 gcataaagtg taaagcctgg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
       61 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      121 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgtgatggg ggatccacta
      181 gttctagaat ccgtcgaaac taagttctgg tgttttaaaa ctaaaaaaaa gactaactat
      241 aaaagtagaa tttaagaagt ttaagaaata gatttacaga attacaatca atacctaccg
      301 tctttatata cttattagtc aagtagggga ataatttcag ggaactggtt tcaacctttt
      361 ttttcagctt tttccaaatc agagagagca gaaggtaata gaaggtgtaa gaaaatgaga
      421 tagatacatg cgtgggtcaa ttgccttgtg tcatcattta ctccaggcag gttgcatcac
      481 tccattgagg ttgtgcccgt tttttgcctg tttgtgcccc tgttctctgt agttgcgcta
      541 agagaatgga cctatgaact gatggttggt gaagaaaaca atattttggt gctgggattc
      601 ttttttttt
//
