LOCUS Exported 13403 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION FRET/FLIM analysis in plants (stable or transient transformation). ACCESSION . VERSION . KEYWORDS pFRETtv-2in1-NN SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13403) AUTHORS Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C TITLE Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic Protein Interaction Studies. JOURNAL Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. Epub 2015 May 13. PUBMED 25971551 REFERENCE 2 (bases 1 to 13403) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13403 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 143..487 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" CDS 575..1291 /codon_start=1 /product="Venus YFP with monomerizing A206K mutation (Nagai et al., 2002; Kremers et al., 2006)" /label=mVenus /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" protein_bind 1310..1434 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 1459..1489 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 1543..2202 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1609..1628) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 2544..2849 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 2728..2747 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(2890..3014) /gene="mutant version of attR" /label=attR4 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 3040..3069 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 3070..3099 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" promoter 3440..3784 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" CDS 3872..4588 /codon_start=1 /product="enhanced monomeric variant of CFP (Goedhart et al., 2012)" /label=mTurquoise2 /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" protein_bind 4608..4731 /gene="mutant version of attR" /label=attR3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 4766..4796 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4804..4820 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 4809..4831 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 4828..4844 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 4828..4844 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" protein_bind complement(5105..5229) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 5246..5272 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 5276..5302 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 5312..5338 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" terminator 6446..6698 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_feature 6803..6827 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 7348..8139 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 8385..8973 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8874..8893 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9127..9144 /label=L4440 /note="L4440 vector, forward primer" misc_feature 9159..9299 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(9225..9244) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9385..9407 /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 9643..9837 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(9903..10976) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" CDS complement(11405..12034) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" misc_feature 13365..13389 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" ORIGIN 1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagccctc 61 tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag 121 tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc 181 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg 241 gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctaccg 301 acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc 361 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg 421 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg 481 agaggacctc gagcgcgcgt atttttaaac aattaccaac aacaacaaca aacaacaaca 541 acattacatt ttacattcta caactacatc tagtatggtg agcaagggcg aggagctgtt 601 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 661 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg 721 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct 781 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 841 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 901 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 961 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 1021 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg 1081 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat 1141 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag 1201 caaggatccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 1261 gatcactctc ggcatggacg agctgtacaa gagtaacact agtcctagaa caagtttgta 1321 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt 1381 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc 1441 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg 1501 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact 1561 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag 1621 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag 1681 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg 1741 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat 1801 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg 1861 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt 1921 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca 1981 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc 2041 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg 2101 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat 2161 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta 2221 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat 2281 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag 2341 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg 2401 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc 2461 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc 2521 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc 2581 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg 2641 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg 2701 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg 2761 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg 2821 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg 2881 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta 2941 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcaactttt 3001 ctatacaaag ttggtcaatt gttaggcggt ggatctctcg agcagaagtt gatctcagag 3061 gaggacctcg agcagaagtt gatctcagag gaggacttat aagcggccat gctagagtcc 3121 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg 3181 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag 3241 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc 3301 taattcctaa aaccaaaatc cagtgaaggg ctcgcacgtg cggagctccg taccctctgc 3361 aggtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct 3421 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg 3481 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct 3541 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca 3601 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa 3661 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac 3721 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga 3781 ggacctcgag cgcgcgtatt tttaaacaat taccaacaac aacaacaaac aacaacaaca 3841 ttacatttta cattctacaa ctacatctag tatggtgagc aagggcgagg agctgttcac 3901 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 3961 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 4021 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgtcct ggggcgtgca 4081 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 4141 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 4201 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 4261 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactact ttagcgacaa 4321 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca 4381 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 4441 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa 4501 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 4561 cactctcggc atggacgagc tgtacaagag taacactagt caattggcaa ctttgtataa 4621 taaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca 4681 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgcg 4741 ggctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 4801 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat taccccgtcc 4861 ctgcacgcct gtcgttcaac cctggaagat ccgcgtgtcc cgagttctaa cagtctggcc 4921 gtcgttctgc agcgtcgcga ttgggaaaac ccgggcgtga cccaactgaa tcgtctggcg 4981 gcccatccgc cgtttgcgtc gtggcgtaac tcagaagaag cccgtaccga ccgtccgtct 5041 caacaactgc gctcctaaca ctataaaaag ctgatgcaga cggggctttt ttctgcaggt 5101 cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa 5161 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta 5221 caaagtggtg tctaggttac cagtataccc ttatgatgtt cctgattatg ctggttaccc 5281 ttacgatgtt cctgattacg caggatctgt atacccttac gatgttccag attatgccta 5341 agcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct 5401 atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg 5461 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata 5521 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgaagggt agatcatgag 5581 cggagaatta agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac 5641 gtttggaact gacagaaccg caacgttgaa ggagccactg agcgcgggtt tctggagttt 5701 aatgagctaa gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca 5761 ctatcatcta gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct 5821 cggtatccaa ttagagtctc atattcactc tcaactcgat cgaggggatc taccatgagt 5881 ccagaaagga gaccagcaga tattaggaga gcaaccgaag cagatatgcc agcagtttgc 5941 accattgtga accattacat cgagacttct acagttaatt ttaggactga acctcaagag 6001 ccacaggaat ggacagatga tctcgtgaga ttaagggaaa gatacccttg gcttgttgct 6061 gaggtggatg gagaagttgc aggtattgct tacgcaggac cttggaaggc tagaaacgct 6121 tatgattgga ctgctgagtc taccgtttac gtgtcaccta gacatcaaag aaccggactt 6181 ggttcaacct tgtatactca ccttttgaag tctcttgaag ctcagggatt caaatctgtt 6241 gtggctgtta tcggtttgcc taatgatcca agtgtgagaa tgcatgaagc tctcggatac 6301 gcaccaaggg gtatgttaag agctgctgga tttaaacatg gtaactggca cgatgttggt 6361 ttctggcagt tagatttcag tttaccagtt ccaccaagac cagtgcttcc agtgaccgag 6421 atttgacccg ggctagagtc aagcagatcg ttcaaacatt tggcaataaa gtttcttaat 6481 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa 6541 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag 6601 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga 6661 taaattatcg cgcgcggtgt catctatgtt actagatcga ccggcatgaa gctgagttaa 6721 cgatgtaagt cctcaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat 6781 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac 6841 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc 6901 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc 6961 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt 7021 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc 7081 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc 7141 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg 7201 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg 7261 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc 7321 agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact 7381 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta 7441 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg 7501 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg 7561 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt 7621 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa 7681 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg 7741 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag 7801 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg 7861 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc 7921 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg 7981 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat 8041 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac 8101 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc 8161 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat 8221 tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa 8281 gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg 8341 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 8401 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 8461 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 8521 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 8581 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 8641 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 8701 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 8761 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 8821 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 8881 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 8941 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 9001 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 9061 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 9121 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt 9181 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat 9241 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc 9301 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 9361 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 9421 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc 9481 gcggcttgtc cgcgccctgg tagattgcct ggccctaggc cagccatttt tgagcggcca 9541 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta 9601 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg 9661 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct 9721 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg 9781 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga 9841 aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta 9901 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc 9961 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc 10021 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag 10081 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag 10141 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc 10201 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac 10261 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg 10321 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg 10381 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc 10441 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc 10501 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg 10561 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct 10621 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg 10681 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa 10741 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc 10801 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt 10861 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg 10921 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg 10981 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta 11041 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt 11101 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat 11161 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg 11221 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg 11281 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt 11341 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat 11401 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt 11461 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca 11521 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa 11581 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc 11641 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata 11701 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca 11761 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg 11821 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt 11881 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat 11941 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc 12001 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt 12061 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca 12121 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc 12181 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc 12241 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa 12301 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc 12361 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc 12421 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga 12481 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg 12541 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg 12601 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg 12661 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac 12721 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt 12781 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg 12841 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc 12901 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc 12961 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg 13021 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca 13081 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac 13141 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg 13201 aggggttgga tcaaagtact ttaaagtact ttaaagtact ttaaagtact ttgatcccga 13261 ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc 13321 cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat 13381 atcctgtcaa acactgatag ttt //