LOCUS Exported 13356 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION FRET/FLIM analysis in plants (stable or transient transformation). ACCESSION . VERSION . KEYWORDS pFRETgc-2in1-CN SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13356) AUTHORS Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C TITLE Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic Protein Interaction Studies. JOURNAL Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. Epub 2015 May 13. PUBMED 25971551 REFERENCE 2 (bases 1 to 13356) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13356 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 165..509 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" CDS 597..1304 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(742..760) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1010..1029 /label=mCherry-F /note="mCherry, forward primer" protein_bind 1323..1447 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 1472..1502 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 1556..2215 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1622..1641) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 2557..2862 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 2741..2760 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(2903..3027) /gene="mutant version of attR" /label=attR4 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 3053..3082 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 3083..3112 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" promoter 3486..3830 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" protein_bind 3925..4048 /gene="mutant version of attR" /label=attR3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 4083..4113 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4121..4137 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 4126..4148 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 4145..4161 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 4145..4161 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" protein_bind complement(4422..4546) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 4566..5288 /codon_start=1 /product="enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYKL" primer_bind complement(4611..4632) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4872..4891) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5219..5240 /label=EGFP-C /note="EGFP, forward primer" terminator 6399..6651 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_feature 6756..6780 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 7301..8092 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 8338..8926 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8827..8846 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9080..9097 /label=L4440 /note="L4440 vector, forward primer" misc_feature 9112..9252 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(9178..9197) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9338..9360 /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 9596..9790 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(9856..10929) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" CDS complement(11358..11987) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" misc_feature 13318..13342 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" ORIGIN 1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagcaacg 61 acggccagtg aattcaggcc tctgcaggtc aacatggtgg agcacgacac tctcgtctac 121 tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa 181 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa 241 aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct 301 atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac 361 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc 421 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata 481 taaggaagtt catttcattt ggagaggacc tcgagcgcgc gtatttttaa acaattacca 541 acaacaacaa caaacaacaa caacattaca ttttacattc tacaactaca tctagtatgg 601 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc 661 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 721 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg 781 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg 841 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 901 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg 961 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc 1021 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc 1081 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg 1141 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca 1201 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg 1261 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagagtaac actagtccta 1321 gaacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat 1381 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt 1441 cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa 1501 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga 1561 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt 1621 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac 1681 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat 1741 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct 1801 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt 1861 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca 1921 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat 1981 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa 2041 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa 2101 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg 2161 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaagatc 2221 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg 2281 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag 2341 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg 2401 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg 2461 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga 2521 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat 2581 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc 2641 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt 2701 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg 2761 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat 2821 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata 2881 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt 2941 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc 3001 tcgttcaact tttctataca aagttggtca attgttaggc ggtggatctc tcgagcagaa 3061 gttgatctca gaggaggacc tcgagcagaa gttgatctca gaggaggact tataagcggc 3121 catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt 3181 ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg 3241 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta 3301 tcaataaaat ttctaattcc taaaaccaaa atccagtgaa ggcctaagct tggcgtaatc 3361 atggtcatag ctgttgctcg cacgtgcgga gctccgtacc ctctgcaggt caacatggtg 3421 gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccagagg 3481 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca 3541 gctatctgtc acttcatcga aaggacagta gaaaaggaag atggcttcta caaatgccat 3601 cattgcgata aaggaaaggc tatcgttcaa gatgcctcta ccgacagtgg tcccaaagat 3661 ggacccccac ccacgaggaa catcgtggaa aaagaagacg ttccaaccac gtcttcaaag 3721 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct 3781 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac ctcgagcgcg 3841 cgtattttta aacaattacc aacaacaaca acaaacaaca acaacattac attttacatt 3901 ctacaactac atctagtcaa ttggcaactt tgtataataa agttgaacga gaaacgtaaa 3961 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact 4021 gtaaaacaca acatatccag tcactatggc ggccgcgggc tcactcatta ggcaccccag 4081 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt 4141 cacacaggaa acagctatga ccatgattac cccgtccctg cacgcctgtc gttcaaccct 4201 ggaagatccg cgtgtcccga gttctaacag tctggccgtc gttctgcagc gtcgcgattg 4261 ggaaaacccg ggcgtgaccc aactgaatcg tctggcggcc catccgccgt ttgcgtcgtg 4321 gcgtaactca gaagaagccc gtaccgaccg tccgtctcaa caactgcgct cctaacacta 4381 taaaaagctg atgcagacgg ggcttttttc tgcaggtcga ccatagtgac tggatatgtt 4441 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat 4501 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgtct aggttaggca 4561 ctagtatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 4621 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 4681 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 4741 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca 4801 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 4861 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 4921 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 4981 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga 5041 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc 5101 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 5161 accactacct gagtactcag tccaagctga gcaaagaccc caacgagaag cgcgatcaca 5221 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 5281 agttataagc ggccatgcta gagtccgcaa aaatcaccag tctctctcta caaatctatc 5341 tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc 5401 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg 5461 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctaggag 5521 ggtagatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga 5581 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctgagcgcgg 5641 gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg cgttcaaaag 5701 tcgcctaagg tcactatcat ctagcaaata tttcttgtca aaaatgctcc actgacgttc 5761 cataaattcc cctcggtatc caattagagt ctcatattca ctctcaactc gatcgagggg 5821 atctaccatg agtccagaaa ggagaccagc agatattagg agagcaaccg aagcagatat 5881 gccagcagtt tgcaccattg tgaaccatta catcgagact tctacagtta attttaggac 5941 tgaacctcaa gagccacagg aatggacaga tgatctcgtg agattaaggg aaagataccc 6001 ttggcttgtt gctgaggtgg atggagaagt tgcaggtatt gcttacgcag gaccttggaa 6061 ggctagaaac gcttatgatt ggactgctga gtctaccgtt tacgtgtcac ctagacatca 6121 aagaaccgga cttggttcaa ccttgtatac tcaccttttg aagtctcttg aagctcaggg 6181 attcaaatct gttgtggctg ttatcggttt gcctaatgat ccaagtgtga gaatgcatga 6241 agctctcgga tacgcaccaa ggggtatgtt aagagctgct ggatttaaac atggtaactg 6301 gcacgatgtt ggtttctggc agttagattt cagtttacca gttccaccaa gaccagtgct 6361 tccagtgacc gagatttgac ccgggctaga gtcaagcaga tcgttcaaac atttggcaat 6421 aaagtttctt aatattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt 6481 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg 6541 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc 6601 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgaccggcat 6661 gaagctgagt taacgatgta agtcctcaat tcggcgttaa ttcagtacat taaaaacgtc 6721 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca 6781 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc 6841 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc 6901 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga 6961 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaat 7021 tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg 7081 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt 7141 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc 7201 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt 7261 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc 7321 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac 7381 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga 7441 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat 7501 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga 7561 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact 7621 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat 7681 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg 7741 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa 7801 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt 7861 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt 7921 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc 7981 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga 8041 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctagc 8101 tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca 8161 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa 8221 gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca aaatccctta 8281 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 8341 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 8401 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 8461 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 8521 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 8581 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 8641 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 8701 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 8761 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 8821 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 8881 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 8941 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 9001 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 9061 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg 9121 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac 9181 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg 9241 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg 9301 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg 9361 ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc 9421 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggcccta ggccagccat 9481 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag 9541 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc 9601 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc 9661 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt 9721 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt 9781 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag 9841 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg 9901 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc 9961 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc 10021 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg 10081 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac 10141 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc 10201 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa 10261 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat 10321 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc 10381 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc 10441 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc 10501 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc 10561 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga 10621 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag 10681 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc 10741 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga 10801 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa 10861 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg 10921 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca 10981 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg 11041 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta 11101 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc 11161 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg 11221 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc 11281 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt 11341 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc 11401 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg 11461 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc 11521 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag 11581 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg 11641 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc 11701 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg 11761 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt 11821 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg 11881 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc 11941 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc 12001 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt 12061 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg 12121 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg 12181 ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct 12241 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc 12301 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa 12361 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta 12421 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg 12481 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa 12541 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga 12601 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt 12661 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc 12721 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac 12781 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt 12841 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg 12901 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc 12961 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact 13021 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta 13081 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc 13141 actatagcag cggaggggtt ggatcaaagt actttaaagt actttaaagt actttaaagt 13201 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa 13261 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt 13321 tacccgccaa tatatcctgt caaacactga tagttt //