LOCUS Exported 11122 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION transient expression of dCas9-LSD1 fusion protein. ACCESSION . VERSION . KEYWORDS p-dCas9-LSD1-Hygro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11122) AUTHORS Stephan Beck TITLE Beck lab Cas9 plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 11122) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11122 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 327..350 /label=MSCV-rev /note="Murine stem cell virus, reverse primer" enhancer 390..769 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 770..973 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 924..944 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 1015..1034 /label=T7 /note="T7 promoter, forward primer" promoter 1015..1033 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 1041..1050 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1047..5150 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 5157..5177 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5184..5249 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" primer_bind complement(7335..7352) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7341..7565 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(7636..7652) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7636..7652) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7649..7671) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7660..7676 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7684..7714) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7729..7750 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7867..7884) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8038..8626) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8118..8137) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8797..9657) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9420..9439 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9658..9762) /gene="bla" /label=AmpR promoter polyA_signal 9813..9934 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9824..9843) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 9878..9897 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(9965..10990) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" promoter complement(11008..385) /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(11056..11075) /label=mPGK-F /note="Mouse PGK promoter, forward primer" ORIGIN 1 cccctgagcc cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag 61 caaagctgct attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg 121 tccatctgca cgagactagt gagacgtgct acttccattt gtcacgtcct gcacgacgcg 181 agctgcgggg cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg 241 ccaccaaaga acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag 301 aggccacttg tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc 361 cttgggaaaa gcgcctcccc tacccggttg acattgatta ttgactagtt attaatagta 421 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac 481 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac 541 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt 601 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat 661 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga 721 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt 781 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca 841 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg 901 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta 961 tataagcaga gctggtttag tgaaccgtca gatccgctag agatccgcgg ccgctaatac 1021 gactcactat agggagagcc gccaccatgg ataaaaagta ttctattggt ttagccatcg 1081 gcactaattc cgttggatgg gctgtcataa ccgatgaata caaagtacct tcaaagaaat 1141 ttaaggtgtt ggggaacaca gaccgtcatt cgattaaaaa gaatcttatc ggtgccctcc 1201 tattcgatag tggcgaaacg gcagaggcga ctcgcctgaa acgaaccgct cggagaaggt 1261 atacacgtcg caagaaccga atatgttact tacaagaaat ttttagcaat gagatggcca 1321 aagttgacga ttctttcttt caccgtttgg aagagtcctt ccttgtcgaa gaggacaaga 1381 aacatgaacg gcaccccatc tttggaaaca tagtagatga ggtggcatat catgaaaagt 1441 acccaacgat ttatcacctc agaaaaaagc tagttgactc aactgataaa gcggacctga 1501 ggttaatcta cttggctctt gcccatatga taaagttccg tgggcacttt ctcattgagg 1561 gtgatctaaa tccggacaac tcggatgtcg acaaactgtt catccagtta gtacaaacct 1621 ataatcagtt gtttgaagag aaccctataa atgcaagtgg cgtggatgcg aaggctattc 1681 ttagcgcccg cctctctaaa tcccgacggc tagaaaacct gatcgcacaa ttacccggag 1741 agaagaaaaa tgggttgttc ggtaacctta tagcgctctc actaggcctg acaccaaatt 1801 ttaagtcgaa cttcgactta gctgaagatg ccaaattgca gcttagtaag gacacgtacg 1861 atgacgatct cgacaatcta ctggcacaaa ttggagatca gtatgcggac ttatttttgg 1921 ctgccaaaaa ccttagcgat gcaatcctcc tatctgacat actgagagtt aatactgaga 1981 ttaccaaggc gccgttatcc gcttcaatga tcaaaaggta cgatgaacat caccaagact 2041 tgacacttct caaggcccta gtccgtcagc aactgcctga gaaatataag gaaatattct 2101 ttgatcagtc gaaaaacggg tacgcaggtt atattgacgg cggagcgagt caagaggaat 2161 tctacaagtt tatcaaaccc atattagaga agatggatgg gacggaagag ttgcttgtaa 2221 aactcaatcg cgaagatcta ctgcgaaagc agcggacttt cgacaacggt agcattccac 2281 atcaaatcca cttaggcgaa ttgcatgcta tacttagaag gcaggaggat ttttatccgt 2341 tcctcaaaga caatcgtgaa aagattgaga aaatcctaac ctttcgcata ccttactatg 2401 tgggacccct ggcccgaggg aactctcggt tcgcatggat gacaagaaag tccgaagaaa 2461 cgattactcc atggaatttt gaggaagttg tcgataaagg tgcgtcagct caatcgttca 2521 tcgagaggat gaccaacttt gacaagaatt taccgaacga aaaagtattg cctaagcaca 2581 gtttacttta cgagtatttc acagtgtaca atgaactcac gaaagttaag tatgtcactg 2641 agggcatgcg taaacccgcc tttctaagcg gagaacagaa gaaagcaata gtagatctgt 2701 tattcaagac caaccgcaaa gtgacagtta agcaattgaa agaggactac tttaagaaaa 2761 ttgaatgctt cgattctgtc gagatctccg gggtagaaga tcgatttaat gcgtcacttg 2821 gtacgtatca tgacctccta aagataatta aagataagga cttcctggat aacgaagaga 2881 atgaagatat cttagaagat atagtgttga ctcttaccct ctttgaagat cgggaaatga 2941 ttgaggaaag actaaaaaca tacgctcacc tgttcgacga taaggttatg aaacagttaa 3001 agaggcgtcg ctatacgggc tggggacgat tgtcgcggaa acttatcaac gggataagag 3061 acaagcaaag tggtaaaact attctcgatt ttctaaagag cgacggcttc gccaatagga 3121 actttatgca gctgatccat gatgactctt taaccttcaa agaggatata caaaaggcac 3181 aggtttccgg acaaggggac tcattgcacg aacatattgc gaatcttgct ggttcgccag 3241 ccatcaaaaa gggcatactc cagacagtca aagtagtgga tgagctagtt aaggtcatgg 3301 gacgtcacaa accggaaaac attgtaatcg agatggcacg cgaaaatcaa acgactcaga 3361 aggggcaaaa aaacagtcga gagcggatga agagaataga agagggtatt aaagaactgg 3421 gcagccagat cttaaaggag catcctgtgg aaaataccca attgcagaac gagaaacttt 3481 acctctatta cctacaaaat ggaagggaca tgtatgttga tcaggaactg gacataaacc 3541 gtttatctga ttacgacgtc gatgccattg taccccaatc ctttttgaag gacgattcaa 3601 tcgacaataa agtgcttaca cgctcggata agaaccgagg gaaaagtgac aatgttccaa 3661 gcgaggaagt cgtaaagaaa atgaagaact attggcggca gctcctaaat gcgaaactga 3721 taacgcaaag aaagttcgat aacttaacta aagctgagag gggtggcttg tctgaacttg 3781 acaaggccgg atttattaaa cgtcagctcg tggaaacccg ccaaatcaca aagcatgttg 3841 cacagatact agattcccga atgaatacga aatacgacga gaacgataag ctgattcggg 3901 aagtcaaagt aatcacttta aagtcaaaat tggtgtcgga cttcagaaag gattttcaat 3961 tctataaagt tagggagata aataactacc accatgcgca cgacgcttat cttaatgccg 4021 tcgtagggac cgcactcatt aagaaatacc cgaagctaga aagtgagttt gtgtatggtg 4081 attacaaagt ttatgacgtc cgtaagatga tcgcgaaaag cgaacaggag ataggcaagg 4141 ctacagccaa atacttcttt tattctaaca ttatgaattt ctttaagacg gaaatcactc 4201 tggcaaacgg agagatacgc aaacgacctt taattgaaac caatggggag acaggtgaaa 4261 tcgtatggga taagggccgg gacttcgcga cggtgagaaa agttttgtcc atgccccaag 4321 tcaacatagt aaagaaaact gaggtgcaga ccggagggtt ttcaaaggaa tcgattcttc 4381 caaaaaggaa tagtgataag ctcatcgctc gtaaaaagga ctgggacccg aaaaagtacg 4441 gtggcttcga tagccctaca gttgcctatt ctgtcctagt agtggcaaaa gttgagaagg 4501 gaaaatccaa gaaactgaag tcagtcaaag aattattggg gataacgatt atggagcgct 4561 cgtcttttga aaagaacccc atcgacttcc ttgaggcgaa aggttacaag gaagtaaaaa 4621 aggatctcat aattaaacta ccaaagtata gtctgtttga gttagaaaat ggccgaaaac 4681 ggatgttggc tagcgccgga gagcttcaaa aggggaacga actcgcacta ccgtctaaat 4741 acgtgaattt cctgtattta gcgtcccatt acgagaagtt gaaaggttca cctgaagata 4801 acgaacagaa gcaacttttt gttgagcagc acaaacatta tctcgacgaa atcatagagc 4861 aaatttcgga attcagtaag agagtcatcc tagctgatgc caatctggac aaagtattaa 4921 gcgcatacaa caagcacagg gataaaccca tacgtgagca ggcggaaaat attatccatt 4981 tgtttactct taccaacctc ggcgctccag ccgcattcaa gtattttgac acaacgatag 5041 atcgcaaacg atacacttct accaaggagg tgctagacgc gacactgatt caccaatcca 5101 tcacgggatt atatgaaact cggatagatt tgtcacagct tgggggtgac ggatccccca 5161 agaagaagag gaaagtctcg agcgactaca aagaccatga cggtgattat aaagatcatg 5221 acatcgatta caaggatgac gatgacaagg ctgcaggagg cggaggtagc ccatcgggtg 5281 tggagggcgc agctttccag agccgacttc ctcatgaccg gatgacttct caagaagcag 5341 cctgttttcc agatattatc agtggaccac aacagaccca gaaggttttt cttttcatta 5401 gaaaccgcac actgcagttg tggttggata atccaaagat tcagctgaca tttgaggcta 5461 ctctccaaca attagaagca ccttataaca gtgatactgt gcttgtccac cgagttcaca 5521 gttatttaga gcgtcatggt cttatcaact tcggcatcta taagaggata aaacccctac 5581 caactaaaaa gacaggaaag gtaattatta taggctctgg ggtctcaggc ttggcagcag 5641 ctcgacagtt acaaagtttt ggaatggatg tcacactttt ggaagccagg gatcgtgtgg 5701 gtggacgagt tgccacattt cgcaaaggaa actatgtagc tgatcttgga gccatggtgg 5761 taacaggtct tggagggaat cctatggctg tggtcagcaa acaagtaaat atggaactgg 5821 ccaagatcaa gcaaaaatgc ccactttatg aagccaacgg acaagctgtt cctaaagaga 5881 aagatgaaat ggtagagcaa gagtttaacc ggttgctaga agctacatct taccttagtc 5941 atcaactaga cttcaatgtc ctcaataata agcctgtgtc ccttggccag gcattggaag 6001 ttgtcattca gttacaagag aagcatgtca aagatgagca gattgaacat tggaagaaga 6061 tagtgaaaac tcaggaagaa ttgaaagaac ttcttaataa gatggtaaat ttgaaagaga 6121 aaattaaaga actccatcag caatacaaag aagcatctga agtaaagcca cccagagata 6181 ttactgccga gttcttagtg aaaagcaaac acagggatct gaccgcccta tgcaaggaat 6241 atgatgaatt agctgaaaca caaggaaagc tagaagaaaa acttcaggag ttggaagcga 6301 atcccccaag tgatgtatat ctctcatcaa gagacagaca aatacttgat tggcattttg 6361 caaatcttga atttgctaat gccacacctc tctcaactct ctcccttaag cactgggatc 6421 aggatgatga ctttgagttc actggcagcc acctgacagt aaggaatggc tactcgtgtg 6481 tgcctgtggc tttagcagaa ggcctagaca ttaaactgaa tacagcagtg cgacaggttc 6541 gctacacggc ttcaggatgt gaagtgatag ctgtgaatac ccgctccacg agtcaaacct 6601 ttatttataa atgcgacgca gttctctgta cccttcccct gggtgtgctg aagcagcagc 6661 caccagccgt tcagtttgtg ccacctctcc ctgagtggaa aacatctgca gtccaaagga 6721 tgggatttgg caaccttaac aaggtggtgt tgtgttttga tcgggtgttc tgggatccaa 6781 gtgtcaattt gttcgggcat gttggcagta cgactgccag caggggtgag ctcttcctct 6841 tctggaacct ctataaagct ccaatactgt tggcactagt ggcaggagaa gctgctggta 6901 tcatggaaaa cataagtgac gatgtgattg ttggccgatg cctggccatt ctcaaaggga 6961 tttttggtag cagtgcagta cctcagccca aagaaactgt ggtgtctcgt tggcgtgctg 7021 atccctgggc tcggggctct tattcctatg ttgctgcagg atcatctgga aatgactatg 7081 atttaatggc tcagccaatc actcctggcc cctcgattcc aggtgcccca cagccgattc 7141 cacgactctt ctttgcggga gaacatacga tccgtaacta cccagccaca gtgcatggtg 7201 ctctgctgag tgggctgcga gaagcgggaa gaattgcaga ccagtttttg ggggccatgt 7261 atacgctgcc tcgccaggcc acaccaggtg ttcctgcaca gcagtcccca agcatgtgaa 7321 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 7381 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 7441 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 7501 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc 7561 tatggcttct gaggcggaaa gaaccagctg gggctcgata ccgtcgacct ctagctagag 7621 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 7681 acacaacata cgagccggaa gcataaagtg taaagcctag ggtgcctaat gagtgagcta 7741 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 7801 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 7861 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 7921 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 7981 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 8041 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 8101 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 8161 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 8221 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 8281 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 8341 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 8401 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 8461 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 8521 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 8581 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 8641 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 8701 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 8761 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 8821 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 8881 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 8941 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 9001 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 9061 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 9121 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 9181 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 9241 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 9301 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 9361 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 9421 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 9481 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 9541 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 9601 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 9661 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 9721 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 9781 gccacctgac gtcgacgggt atacagacat gataagatac attgatgagt ttggacaaac 9841 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt 9901 atttgtaacc attataagct gcaataaaca agttggggtg ggcgaagaac tctcggcatc 9961 tactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta 10021 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga 10081 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat 10141 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg 10201 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct 10261 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat 10321 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga 10381 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc 10441 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag 10501 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt 10561 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg 10621 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag 10681 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc 10741 tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat 10801 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac 10861 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt 10921 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag 10981 gctttttcat ggtggcggga tgcaggtcga aaggcccgga gatgaggaag aggagaacag 11041 cgcggcagac gtgcgctttt gaagcgtgca gaatgccggg cctccggagg accttcgggc 11101 gcccgccccg cccctgagcc cg //